本文選題：HIV-1 + 修飾型gagpo(lmodgagpol)　； 參考：《吉林大學(xué)》2006年碩士論文

【摘要】：人類免疫缺陷病毒(HIV)是逆轉(zhuǎn)錄病毒科的一個(gè)毒種,它主要引起艾滋病,即獲得性免疫缺陷綜合征(acquired immunodeficiency syndrome,AIDS),這是一種以全身免疫系統(tǒng)嚴(yán)重?fù)p害為主要特征的傳染性疾病。雖然近幾年抗艾滋病藥物的“雞尾酒”療法在發(fā)達(dá)國(guó)家有效地控制了HIV的蔓延,但是昂貴的價(jià)格,耐藥病毒株的產(chǎn)生,長(zhǎng)期用藥的副作用以及最終無(wú)法徹底清除患者體內(nèi)病毒等方面的不利因素表明,只有艾滋病疫苗才能真正有效地預(yù)防和控制艾滋病。所以艾滋病疫苗的研究勢(shì)在必行。 HIV的結(jié)構(gòu)基因gagpol(wtgagpol)是HIV基因組中比較保守的區(qū)域,也是我們選擇抗原的主要對(duì)象。在我們的工作中,首先為了提高產(chǎn)物蛋白的表達(dá)量,我們消除了一些抑制HIV-gag-pol表達(dá)的因子,構(gòu)建了密碼子優(yōu)化過(guò)的gagpol(modgagpol)基因,為了研究wtgagpol和modgagpol在實(shí)驗(yàn)動(dòng)物體內(nèi)誘導(dǎo)的免疫應(yīng)答水平,我們選擇了重組復(fù)制缺陷型腺病毒5型(rAdV-5)作為載體將wtgagpol及modgagpol基因?qū)雱?dòng)物體內(nèi),并探討了兩者體液免疫及細(xì)胞免疫的差別。以上兩種疫苗的應(yīng)用和免疫方案的研究,為我們進(jìn)一步探索利用重組腺病毒載體來(lái)研究HIV-1疫苗打下了基礎(chǔ)。
[Abstract]:Human immunodeficiency virus (HIV) is a virus of the family retroviridae. It mainly causes AIDS, or acquired immunodeficiency syndrome (acquired immunodeficiency syndrome), which is a infectious disease characterized by serious damage to the systemic immune system. Although the "cocktail" therapy of anti-AIDS drugs has effectively controlled the spread of HIV in developed countries in recent years, the high price, the production of drug-resistant strains of the virus, The side effects of long-term drug use and the unfavorable factors, such as the failure to eliminate the virus in patients' bodies, indicate that only AIDS vaccine can effectively prevent and control AIDS. Therefore, it is imperative to study HIV vaccine. HIV structural gene gagpol (wtgagpol) is a conservative region of the HIV genome, and is also the main target of our selection of antigens. In our work, first of all, in order to increase the expression of the product protein, we eliminated some factors that inhibit the expression of HIV-1 gag-pol, and constructed the codon optimized gagpol (modgagpol) gene. In order to study the level of immune response induced by wtgagpol and modgagpol in experimental animals, We selected recombinant replication-deficient adenovirus type 5 (rAdV-5) as a vector to transfer wtgagpol and modgagpol genes into animals, and discussed the difference between humoral immunity and cellular immunity. The application of the above two vaccines and the study of immunization schemes have laid a foundation for further exploring and using recombinant adenovirus vectors to study HIV-1 vaccine.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

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本文編號(hào)：2106013