本文選題：石杉?jí)A甲 + 單克隆抗體�。� 參考：《中國(guó)免疫學(xué)雜志》2014年01期

【摘要】：目的:制備并鑒定石杉?jí)A甲單克隆抗體,研制石杉?jí)A甲間接競(jìng)爭(zhēng)酶聯(lián)免疫試劑盒。方法:以石杉?jí)A甲人工抗原免疫BALB/c小鼠獲得單克隆抗體,采用酶聯(lián)免疫吸附法制備石杉?jí)A甲試劑盒,對(duì)試劑盒各項(xiàng)指標(biāo)進(jìn)行評(píng)價(jià)。結(jié)果:試劑盒工作范圍為5~2 500 ng/ml,檢測(cè)限為6.242 ng/ml,與多種生物堿均無(wú)交叉反應(yīng),試劑盒可在4℃或-20℃下穩(wěn)定貯存半年以上,石杉?jí)A甲片回收率在94.2%~108.6%之間,變異系數(shù)在2.3%~4.8%之間。結(jié)論:石杉?jí)A甲ELISA試劑盒快速、靈敏、穩(wěn)定,可用于石杉?jí)A甲的含量測(cè)定。
[Abstract]:Aim: to prepare and identify monoclonal antibody against Huperzine A and to develop an indirect competitive enzyme linked immunosorbent kit (Elisa) for Huperzine A. Methods: monoclonal antibodies were obtained from BALB / c mice immunized with Huperzine A artificial antigen, and Huperzine A kit was prepared by enzyme-linked immunosorbent assay (Elisa). Results: the working range of the kit was 5 ~ 2 500 ng / ml, the detection limit was 6.242 ng / ml, and there was no cross reaction with a variety of alkaloids. The kit could be stably stored at 4 鈩，

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