本文選題：抗原抗體親和力 + 動力學(xué)　； 參考：《福州大學(xué)》2005年碩士論文

【摘要】：抗體是抗原刺激人或動物機體的免疫系統(tǒng)后,由B 淋巴細胞系的終末分化細胞──漿細胞產(chǎn)生的、可與抗原特異性結(jié)合的免疫球蛋白。本課題使用牛血清白蛋白(BSA)作為抗原,通過對小鼠和白兔進行連續(xù)多次免疫和間隔長久免疫,分析抗原抗體親和特性,探討不同免疫周期下抗體產(chǎn)生及親和力成熟的動力學(xué)變化規(guī)律。 伴隨免疫次數(shù)的增加,機體中抗體效價不斷增強,連續(xù)出現(xiàn)的抗原信號使機體中的抗體始終維持在一個較高的水平。使用我們自己建立的尿素洗脫的ELISA 方法,對不同免疫周期下抗體的親和常數(shù)進行計算,表明抗體親和力水平在二次免疫應(yīng)答時存在一個明顯的突變過程�？贵w親和常數(shù)從1.6×108L/mol 上升到6.9×108L/mol。隨后免疫次數(shù)雖然繼續(xù)增加,但親和力變化趨于穩(wěn)定,最終穩(wěn)定于7.2×108L/mol。 通過比較連續(xù)刺激與間隔長久后再次刺激動物時其體內(nèi)抗體親和力變化規(guī)律,發(fā)現(xiàn)機體中存留的記憶細胞是那些在免疫應(yīng)答過程能分泌高親和力抗體的效應(yīng)細胞的后代,其特異性親和力始終維持在一個較高的水平。同時,機體中B 細胞表面受體BCR 雖然其本身并不發(fā)生突變,但伴隨免疫次數(shù)的增加,B 細胞對抗原的親和力也會逐漸增強,三次免疫后經(jīng)過篩選后的B 細胞的BCR 與初次免疫相比,其相對親和常數(shù)比初次免疫時提高了5倍。表明機體內(nèi)對記憶性B 細胞也存在一個連續(xù)篩選的過程。 應(yīng)用毛細管電泳技術(shù)測定不同抗原表位之間的親和力變化,通過計算不同免疫周期下不同表位之間的親和常數(shù)的變化,分析免疫應(yīng)答過程中優(yōu)勢表位的物理特性。分別比較線性表位與空間表位對抗體親和力成熟變化的貢獻,表明空間表位具有一定的競爭優(yōu)勢。線性表位與抗體的親和力從初次免疫時占平均水平(整體表位)親和力的75%降低至四次免疫后的69%。 本課題的研究對于研究疫苗、抗體的作用機制提供了一定的理論依據(jù),對以表位為基礎(chǔ)的現(xiàn)代疫苗的設(shè)計具有一定的推動意義。
[Abstract]:Antibodies are immunoglobulins that specifically bind to antigens that stimulate the immune system of humans or animals and are produced by terminal differentiation cells of the B lymphocyte line, plasma cells. In this study, bovine serum albumin (BSA) was used as antigen to analyze the affinity of antigen-antibody to mice and rabbits by repeated immunization and long-interval immunization. To investigate the dynamic changes of antibody production and affinity maturation in different immune cycles. Along with the increase of immunization times, the titer of antibodies in the body is increasing, and the continuous appearance of antigen signals keeps the antibody in the body at a high level. Using the Urea elution Elisa established by ourselves, the affinity constants of antibodies in different immune cycles were calculated. The results showed that there was an obvious mutation process in the secondary immune response. The antibody affinity constant increased from 1.6 脳 10 ~ (8) L / mol to 6.9 脳 10 ~ (8) L / mol. After that, the number of immunizations continued to increase, but the change of affinity tended to be stable, and finally stabilized at 7.2 脳 10 ~ (8) L / mol. By comparing the changes of antibody affinity between continuous stimulation and repeated stimulation of animals at long intervals, it was found that the memory cells in the body were the offspring of the effector cells that secreted high affinity antibodies during the immune response. Its specific affinity is always maintained at a high level. At the same time, although the B cell surface receptor BCR itself does not mutate, the affinity of B cell to antigen increases with the increase of immune times. The BCR of B cell screened after three times of immunization is compared with that of primary immunization. Its relative affinity constant was 5 times higher than that of primary immunization. It suggests that there is also a continuous screening process for memory B cells in the body. Capillary electrophoresis (CE) was used to determine the affinities of different antigenic epitopes. The physical properties of dominant epitopes in immune response were analyzed by calculating the affinity constants of different epitopes in different immune cycles. The contributions of linear epitopes and spatial epitopes to the mature changes of antibody affinity were compared, which indicated that the spatial epitopes had certain competitive advantages. The affinity of linear epitopes to antibodies decreased from 75% of the average (global epitope) affinity at the time of primary immunization to 69% after four doses of immunization. The research in this paper provides a theoretical basis for the study of vaccine and the mechanism of antibody action, and has a certain significance for the design of modern vaccine based on epitope.
【學(xué)位授予單位】：福州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392

【引證文獻】

相關(guān)碩士學(xué)位論文 前1條

1 蘇森;心肌肌鈣蛋白I光纖生物傳感器研究[D];重慶理工大學(xué);2012年

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本文編號：2100666