本文選題：逼尿肌不穩(wěn)定 + 分布��； 參考：《石河子大學(xué)》2007年碩士論文

【摘要】： 目的:尋找一種簡單、可靠的方法來建立并評估豚鼠膀胱逼尿肌不穩(wěn)定(detrusor instability,DI)模型。觀察與Cajal間質(zhì)細(xì)胞(interstitial cells of Cajal, ICCs)相似的一種細(xì)胞在DI膀胱逼尿肌中的形態(tài)、分布變化,探討其與DI發(fā)病機理的相關(guān)性。 方法:成年雌性豚鼠經(jīng)會陰部不完全尿道結(jié)扎,6周后行膀胱穿刺充盈性膀胱測壓,觀察膀胱壓力、容量變化及逼尿肌不穩(wěn)定的發(fā)生情況。根據(jù)檢測結(jié)果,將建立的實驗豚鼠模型分為DI組和逼尿肌穩(wěn)定(detrusor stabilization,DS)組。另設(shè)正常對照組,采用組織冰凍切片、酪氨酸蛋白激酶受體(receptor tyrosine kinase,KIT)單克隆抗體間接免疫熒光檢測技術(shù)觀察逼尿肌中ICCs樣細(xì)胞的形態(tài)、分布情況。 結(jié)果:①實驗組逼尿肌不穩(wěn)定發(fā)生率35.9%,對照組無發(fā)生,其中DI組膀胱最大壓力和最大容量較對照組顯著增加(p0.01)。②在成年豚鼠膀胱的逼尿肌中存在有ICCs樣細(xì)胞,該細(xì)胞走向與逼尿肌肌束平行,主要分布于逼尿肌肌束邊緣和逼尿肌肌束中,呈梭形雙極細(xì)胞。與對照組比較,DI組ICCs樣細(xì)胞數(shù)量明顯增多。 結(jié)論:①經(jīng)會陰部尿道結(jié)扎、膀胱穿刺充盈性膀胱測壓是建立及評估豚鼠膀胱出口梗阻引起逼尿肌不穩(wěn)定模型的一種簡單、可靠的方法。②KIT單克隆抗體間接免疫熒光檢測技術(shù)是有效觀察膀胱ICCs樣細(xì)胞的免疫組織化學(xué)實驗方法。③DI膀胱中ICCs樣細(xì)胞增多提示其可能導(dǎo)致逼尿肌自發(fā)收縮活動增加,可能就是理論上存在于膀胱的起搏細(xì)胞,可能是DI發(fā)生的重要因素之一。
[Abstract]:Objective: to find a simple and reliable method to establish and evaluate detrusor instabilities DI model in guinea pigs. The morphology and distribution of a cell similar to Cajal interstitial cell (interstitial cells of Cajal in DI bladder detrusor muscle were observed, and the relationship between it and the pathogenesis of DI was discussed. Methods: six weeks after incomplete urethral ligation of perineum, the bladder pressure, volume and detrusor instability of adult female guinea pigs were measured by bladder puncture and filling bladder manometry. According to the test results, the guinea pig model was divided into DI group and detrusor stabilization DS group. The morphology and distribution of ICCS-like cells in detrusor muscle were observed by indirect immunofluorescence assay with monoclonal antibody against tyrosine protein kinase receptor (receptor tyrosine kinaseKit) in normal control group. Results the incidence of detrusor instability was 35.9in the experimental group, but not in the control group. The maximal pressure and volume of bladder in DI group were significantly higher than those in the control group (p0.01) .2 ICCs like cells were found in the detrusor muscle of adult guinea pig bladder. The direction of the cell was parallel to the detrusor bundle, mainly distributed in the edge of the detrusor fascicle and in the detrusor bundle, with fusiform bipolar cells. Compared with the control group, the number of ICCs like cells in DI group was significantly increased. Conclusion it is a simple method to establish and evaluate the model of detrusor instability caused by bladder outlet obstruction in guinea pigs. The reliable method. 2KIT monoclonal antibody indirect immunofluorescence assay is an effective method for observing ICCs like cells in bladder. The increase of ICCs like cells in bladder suggests that the increase of ICCs like cells in bladder may lead to the increase of detrusor spontaneous contraction activity. May be theoretically present in the bladder pacemaker cells, may be one of the important factors of DI.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R-332;R694.54

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