本文選題：白塞病 + T淋巴細(xì)胞　； 參考：《華中科技大學(xué)》2007年博士論文

【摘要】： 目的檢測可誘導(dǎo)共刺激分子(inducible co-stimulator, ICOS)在BD患者外周血T細(xì)胞亞群的表達(dá),探索其與BD疾病活動期和BD穩(wěn)定期的關(guān)系,為進一步研究ICOS在BD免疫病理中的作用奠定基礎(chǔ);我們還檢測了BD患者外周血INF-γ和IL-4的分泌水平,以便進一步了解BD患者外周血Th1/Th2以及Tc1/Tc2細(xì)胞的比例變化,探索BD患者體內(nèi)的免疫狀況。 方法采用流式細(xì)胞術(shù)檢測華中科技大學(xué)同濟醫(yī)學(xué)院附屬協(xié)和醫(yī)院皮膚科門診和住院BD活動期患者(n=22),BD穩(wěn)定期(n=6)以及健康體檢者(n=22)外周血T細(xì)胞亞群表面ICOS的表達(dá)水平以及INF-γ和IL-4的分泌水平,觀察ICOS在不同T細(xì)胞亞群表達(dá)水平與疾病關(guān)系。 結(jié)果1)與正常人相比活動期及穩(wěn)定期BD患者外周血CD4+T細(xì)胞百分比無統(tǒng)計學(xué)差異(P0.05),而CD8+T細(xì)胞則存在顯著差異(P0.05),CD4/CD8比值也明顯降低(P0.05),CD4+CD28+和CD8+CD28+的百分率也明顯下降(P0.05); 2)與正常人相比活動期BD患者外周血CD4+T細(xì)胞和CD8+T細(xì)胞表達(dá)ICOS的水平顯著升高(P0.05),但穩(wěn)定期患者與正常人之間無統(tǒng)計學(xué)差異(P0.05),BD活動期CD28+ICOS+/CD4+、CD28-ICOS+/CD4+和CD28-ICOS+/CD8+與正常人相比顯著升高(P0.05),而BD穩(wěn)定期CD28+ICOS+/CD4+、CD28-ICOS+/CD4+、CD28+ICOS+/CD8+及CD28-ICOS+/CD8+與正常人相比則無顯著性差異(P0.05); 3)BD患者活動期外周血細(xì)胞Th細(xì)胞和Tc細(xì)胞經(jīng)過刺激后胞內(nèi)合成IFN-γ的水平明顯高于正常人水平(p 0.05 ),穩(wěn)定期患者Th細(xì)胞和Tc細(xì)胞體外合成IFN-γ的能力與正常人相比無明顯差異(p0.05),穩(wěn)定期患者Th細(xì)胞和Tc細(xì)胞胞內(nèi)合成IL-4的能力相對于正常人均無明顯差異,但總體水平卻略有上升趨勢(p0.05),而活動期患者Th細(xì)胞和Tc細(xì)胞胞內(nèi)合成IL-4的能力相對于正常人均存在明顯差異(p0.05)。 結(jié)論BD患者外周血某些T細(xì)胞亞群存在ICOS的高表達(dá),INF-γ和IL-4的分泌水平明顯升高,并與疾病活動期存在一定程度相關(guān),因此ICOS可能參與了BD的免疫病理過程。
[Abstract]:Objective to investigate the expression of inducible co-stimulator (inducible co-stimulator) in peripheral blood T cell subsets of BD patients, and to explore the relationship between the expression of inducible co-stimulator and the active phase and stable phase of BD, so as to lay a foundation for further study of the role of inducible in BD immunopathology. We also detected the levels of INF- 緯 and IL-4 in peripheral blood of BD patients in order to further understand the percentage of Th1 / Th2 and Tc1 / Tc2 cells in peripheral blood of BD patients and to explore the immune status of BD patients. Methods flow cytometry was used to detect the expression of ICOS on the surface of peripheral blood T cell subsets in outpatients and in-patients with BD active phase (nnn22) and healthy controls (NN22) in the Department of Dermatology, affiliated Union Medical College, Tongji Medical College, Huazhong University of Science and Technology (Huazhong University of Science and Technology). And the levels of secretion of INF- 緯 and IL-4, To observe the relationship between the expression of ICOS in different T cell subsets and disease. Results 1) there was no significant difference in percentage of CD4 T cells in peripheral blood between active and stable BD patients compared with normal controls (P0.05), while there was a significant difference in CD8 T cells (P0.05). (P0.05) the ratio of CD 4 / CD 8 was also significantly decreased (P0.05) the percentages of CD 4 CD 28 and CD 8 CD 28 in BD patients were also significantly decreased (P 0.05). (P0.05); 2) the expression of ICOS in peripheral blood CD4 T cells and CD8 T cells in active BD patients was significantly higher than that in normal controls (P0.05), but there was no significant difference between stable phase patients and normal controls (P0.05). (P0.05) CD28ICOS / CD4 / CD28-ICOS / CD4 and CD28-ICOS / CD8 / CD8 levels in active BD patients were significantly higher than those in normal controls (P0.05). The level of IFN- 緯 synthesis in peripheral blood cells of BD patients was significantly higher than that in normal controls (P0.05), while CD28ICOS / CD4 + CD28-ICOS / CD4 / CD28 / CD28ICOS / CD8 and CD28-ICOS / CD8 in BD patients were not significantly different from those in normal controls (P0.05); (3) the level of IFN- 緯 synthesis in peripheral blood cells and Tc cells during the active phase of BD patients was significantly higher than that in normal controls (P0.05). The ability of Th cells and Tc cells to synthesize IFN- 緯 in vitro was not significantly different from that of normal controls (p0.05), and the ability of intracellular production of IL-4 in Th cells and Tc cells of stable patients was not significantly different from that of normal individuals. However, the level of IL-4 increased slightly (p0.05), and the ability of IL-4 production in Th cells and Tc cells in active patients was significantly different from that in normal controls (p0.05). Conclusion the high expression of ICOS in some T lymphocyte subsets of patients with BD may increase the secretion of INF- 緯 and IL-4, and may be involved in the immunopathological process of BD.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2007
【分類號】：R597;R392

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 戴冽,尹培達(dá),劉亞光,湯美安;白塞病60例臨床分析[J];風(fēng)濕病學(xué)雜志;1997年04期

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本文編號：2089195