本文選題：UVB + 皮膚移植��； 參考：《西南大學》2007年碩士論文

【摘要】： 背景 異體和異種皮膚是臨床上替代自體皮膚治療大面積燒傷的主要敷料，但異體(異種)皮膚抗原性強，特別是新鮮皮膚，還帶有大量的菌污染，皮膚移植后的強烈免疫排斥反應(yīng)和菌污染往往會導致皮膚過早的壞死。中波紫外線(UVB)，波長280—320nm，它可降低免疫原性，可應(yīng)用于器官移植的前處理，降低受者對移植物的排斥反應(yīng)和移植物的抗宿主反應(yīng)，但UVB是否有滅菌作用，仍未有報道。因此，紫外照射是降低移植皮膚的抗原性、延長移植皮膚存活提供簡單、快捷的途徑。但是將UVB降低免疫原性應(yīng)用于皮膚移植的研究缺乏系統(tǒng)的研究，且多大劑量可以達到最理想的效果仍不清楚。 研究目的和意義 本課題以不同劑量UVB照射離體巴馬香豬皮膚為實驗材料，研究不同劑量UVB對皮膚的免疫原性、帶菌量、活力等指標的影響，旨在尋找可以降低免疫原性、帶菌量少、活力較高的UVB照射劑量，為延長移植皮膚存活奠定理論基礎(chǔ)。 方法與結(jié)果 1．免疫原性檢測 1．1淋巴細胞增殖實驗 UVB以劑量依賴的方式抑制皮膚的免疫原性。30J／cm~2UVB照射時，有輕微抑制，抑制率為14.3％；60J／cm~2UVB照射時，抑制率為42.9％；360J／cm~2UVB照射時，達到最大抑制率，抑制率為99.9％。 1．2 ATP酶染色郎格漢細胞(Langerhans cell，LC) 新鮮皮膚LC ATP酶染色呈棕黑色，樹突狀；UVB處理后，LC樹突減少，甚至消失。UVB處理后LC數(shù)量較新鮮皮有顯著下降，并且隨照射劑量的增加LC數(shù)量逐漸下降。UVB處理后皮膚的免疫原性與皮膚中LC數(shù)量呈正相關(guān)。 2．帶菌量檢測 UVB具有抑菌作用。隨著UVB劑量的增大，皮膚中的帶菌量減少，UVB達到本研究中最大劑量180J／cm~2時，皮膚中仍有帶菌量。 3．UVB對皮膚的損傷作用 用MTT法檢測UVB預(yù)處理的皮膚活性實驗表明，UVB劑量在0—90J／cm~2范圍內(nèi)，皮膚的活力隨劑量的增大而減小，當UVB劑量增加到90—360J／cm~2，皮膚的活力又先升后降。 結(jié)論 UVB具有抑菌作用，可以降低皮膚的免疫原性，但對皮膚有損傷作用；UVB以劑量依賴的方式抑制皮膚的免疫原性，且皮膚的免疫原性與UVB照射后皮膚中LC數(shù)量呈正相關(guān)；UVB劑量為30-60J／cm~2時，離體皮膚免疫原性低，皮膚損傷小，且?guī)Ь康�，可作為異體(異種)皮膚移植的實驗基礎(chǔ)數(shù)據(jù)。
[Abstract]:Background Allogeneic skin and xenogeneic skin are the main dressing to replace autologous skin in treating large area burn, but allogeneic skin has strong antigenicity, especially fresh skin. There is also a large number of bacterial contamination, skin transplantation after strong immune rejection and bacterial contamination will often lead to premature skin necrosis. Ultraviolet B (UVB), wavelength 280-320nm. it can reduce immunogenicity, can be used in the preprocessing of organ transplantation, reduce the graft rejection and graft anti-host reaction, but whether UVB has sterilizing effect has not been reported. Therefore, external violet irradiation is a simple and fast way to reduce the antigenicity of transplanted skin and prolong the survival of transplanted skin. However, the study of reducing immunogenicity of UVB in skin transplantation is lack of systematic research, and it is not clear what dose of UVB can achieve the best effect. Objective and significance to study the effects of different doses of UVB on the immunogenicity, bacterial count and vitality of the skin of Bama Xiang pig irradiated with different doses of UVB as experimental materials, and to study the effects of different doses of UVB on the immunogenicity of skin, the amount of bacteria carried and the activity of the skin. The aim of this study was to find a dose of UVB that could reduce immunogenicity, carry less bacteria and have higher activity, and lay a theoretical foundation for prolonging the survival of transplanted skin. Methods and results 1.Immunogenicity test 1.1 lymphocyte proliferation test UVB Inhibition of skin immunogenicity in a dose-dependent manner. 30 J / cm ~ (2) UVB irradiation, There was slight inhibition, the inhibition rate was 14.3J / cm ~ (-2) UVB irradiation, and the inhibition rate was 42.9% ~ 360J / cm ~ (-2) UVB irradiation, which reached the maximum inhibition rate. The inhibitory rate was 99.9%. 1.2 ATP enzyme staining was found in the fresh skin of Langerhans cellulocytes. LC ATPase staining was brown and black, and the dendritic dendrites decreased after UVB treatment. The number of LC after treatment with UVB was significantly lower than that of fresh skin. The immunogenicity of skin treated with UVB was positively correlated with the number of LC in skin. 2. UVB has bacteriostatic effect. With the increase of UVB dose, the amount of bacteria carried in the skin decreased to the maximum dose of 180 J / cm ~ 2 in this study. 3. The damage effect of UVB on skin. 3. The effect of UVB on skin injury. 3. The skin activity of UVB pretreatment was detected by MTT method. 3. The dose of UVB was measured by MTT assay. In the 0-90 J / cm2 range, The skin activity decreased with the increase of UVB dose. When the UVB dose increased to 90-360 J / cm ~ (2), the skin activity increased first and then decreased. Conclusion UVB can inhibit the immunogenicity of skin, but it can inhibit the immunogenicity of skin in a dose-dependent manner. The immunogenicity of the skin was positively correlated with the amount of LC in the skin after UVB irradiation. The dose of UVB was 30-60 J / cm ~ 2. The immunogenicity of the skin in vitro was low, the skin damage was small, and the amount of bacteria was low, which could be used as the experimental data of xenogeneic skin transplantation.
【學位授予單位】：西南大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R622;R312

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