發(fā)布時(shí)間：2018-06-29 04:42

  本文選題：星形膠質(zhì)細(xì)胞 + 細(xì)胞松弛素��； 參考：《中國(guó)康復(fù)理論與實(shí)踐》2014年07期

【摘要】：目的探討不同濃度細(xì)胞松弛素D(CytD)對(duì)大鼠脊髓星形膠質(zhì)細(xì)胞骨架重構(gòu),水通道蛋白(AQP)1、AQP4及內(nèi)向整流性鉀通道4.1(Kir4.1)基因表達(dá)的影響。方法原代培養(yǎng)大鼠脊髓星形膠質(zhì)細(xì)胞,加CytD 0.05μg/ml、0.10μg/ml、0.20μg/ml、0.40μg/ml、0.80μg/ml和1.00μg/ml共培養(yǎng)2 h、12 h和24 h。MTT法檢測(cè)細(xì)胞增殖情況;鬼筆環(huán)肽聯(lián)合Hoechst 33342套染后激光共聚焦顯微鏡下觀察共培養(yǎng)2 h后細(xì)胞骨架重構(gòu)情況;RT-PCR法檢測(cè)共培養(yǎng)2 h時(shí)AQP1、AQP4和Kir4.1 mRNA表達(dá)水平。結(jié)果細(xì)胞生存率隨CytD濃度升高和作用時(shí)間延長(zhǎng)而減少。CytD使大鼠脊髓星形膠質(zhì)細(xì)胞微絲發(fā)生解聚、彎曲,但極性未失。CytD 0.05μg/ml、0.10μg/ml、0.20μg/ml和0.40μg/ml上調(diào)AQP1、AQP4和Kir4.1 mRNA表達(dá)。結(jié)論適當(dāng)濃度CytD可以重建星形膠質(zhì)細(xì)胞骨架,上調(diào)大鼠脊髓星形膠質(zhì)細(xì)胞AQP1、AQP4和Kir4.1基因表達(dá)。
[Abstract]:Objective to investigate the effects of different concentrations of cytochalasin D (CytD) on cytoskeleton remodeling, aquaporin (AQP) 1, AQP4 and inward rectifier potassium channel 4.1 (Kir4.1) gene expression in rat spinal astrocytes. Methods Primary cultured rat spinal astrocytes were cultured with CytD 0.05 渭 g / ml 0.10 渭 g / ml 0.20 渭 g / ml 0.40 渭 g / ml / ml 0.80 渭 g/ml and 1.00 渭 g/ml for 2 h, 12 h and 24 h, respectively. The cytoskeleton remodeling after 2 h of co-culture was observed under a set of hoechst 33342 sets of laser scanning confocal microscopy. RT-PCR was used to detect the expression levels of AQP1, AQP4 and Kir4.1 mRNA at 2 h after co-culture. Results the cell survival rate decreased with the increase of CytD concentration and the duration of action. CytD induced depolymerization and curvature of microfilaments in rat spinal astrocytes, but the polarity was not lost. CytD 0. 10 渭 g / ml, 0. 20 渭 g/ml and 0. 40 渭 g/ml upregulated the expression of AQP1AQP4 and Kir4.1 mRNA. Conclusion the appropriate concentration of CytD can reconstruct the astrocytoskeleton and up-regulate the expression of AQP1-AQP4 and Kir4.1 genes in rat spinal astrocytes.
【作者單位】： 蘭州大學(xué)第二醫(yī)院骨科;甘肅省骨關(guān)節(jié)病重點(diǎn)實(shí)驗(yàn)室;
【基金】：甘肅省技術(shù)研究與開(kāi)發(fā)專項(xiàng)計(jì)劃項(xiàng)目(No.1004TCYA028) 蘭州大學(xué)第二醫(yī)院2010年度院內(nèi)科研項(xiàng)目(No.YJ2010-48)
【分類號(hào)】：R338
，

本文編號(hào)：2080899