本文選題：可控性心肌缺血 + 動物模型�。� 參考：《南京醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 目的：建立小型豬可控性心肌缺血的動物模型。 材料與方法：健康成年小型豬10只，，雌雄對半。胸壁左側(cè)第四肋間切口開胸安置自制水囊縮窄器，建立鈍緣支可控性心肌缺血的動物模型。術(shù)后行缺血心電圖試驗(yàn)、冠脈造影、微球技術(shù)檢測水囊加壓前后靶血管支配區(qū)局部心肌血流(CCBF)以及心肌HE染色觀察靶血管支配區(qū)形態(tài)學(xué)變化。通過改進(jìn)心電圖導(dǎo)聯(lián)方式、電極粘貼處皮膚處理辦法以獲取較為穩(wěn)定的小型豬運(yùn)動心電圖。 結(jié)果：制作成功的8只小型豬在水囊加壓后均可出現(xiàn)明顯的缺血性心電圖表現(xiàn)，同時冠脈造影顯示鈍緣支下游顯影延遲、稀疏，鈍緣支近乎完全閉塞，水囊壓力撤除后心電圖迅速恢復(fù)正常，鈍緣支遠(yuǎn)端灌注恢復(fù)到水囊加壓前狀態(tài)。水囊加壓前后靶血管支配區(qū)域CCBF明顯減少(p＜0.05)。HE染色顯示靶血管支配區(qū)域心肌無梗死表現(xiàn)。用改進(jìn)的心電圖導(dǎo)聯(lián)方式以及通過細(xì)致的皮膚預(yù)處理，我們獲得了較為穩(wěn)定的運(yùn)動心電圖。 結(jié)論：采用該自制水囊縮窄器制作的可控性心肌缺血動物模型，缺血效果確實(shí)、可靠，可控性強(qiáng)，且動物生存率高，優(yōu)于以往報(bào)道結(jié)果。
[Abstract]:Objective: to establish an animal model of controllable myocardial ischemia in miniature pigs. Materials and methods: 10 healthy adult miniature pigs, half male and female. The fourth intercostal incision on the left side of the chest wall was used to establish an animal model of controlled myocardial ischemia with blunt marginal branch. After operation, ischemic electrocardiogram, coronary angiography and microsphere technique were used to detect the regional myocardial blood flow (CCBF) of the target vascular innervation area (CCBF) and the morphological changes of the target vascular innervation area by HE staining. The electrocardiogram (ECG) of miniature pig was obtained by improving the electrocardiogram (ECG) lead and treating the skin at the electrode. Results: all the 8 miniature pigs had obvious ischemic electrocardiogram (IECG) after water sac compression. Meanwhile, coronary angiography showed that the downstream development of the obtuse marginal branch was delayed, sparse, and the obtuse marginal branch was almost completely occluded. The electrocardiogram returned to normal quickly after the water capsule pressure was removed, and the distal perfusion of the blunt marginal branch returned to the state before the water sac pressure. CCBF in target area decreased significantly before and after water sac compression (p < 0.05). He staining showed that there was no myocardial infarction in target vascular innervation area. By using the improved electrocardiogram (ECG) lead and careful skin pretreatment, we obtained a stable exercise electrocardiogram (EKG). Conclusion: the animal model of controlled myocardial ischemia made by the self-made water capsule constrictor is effective, reliable and controllable, and the survival rate of the animal is high, which is better than that reported in the past.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R-332

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