發(fā)布時(shí)間：2018-06-28 14:29

  本文選題：腦膜炎 + 大腸桿菌　； 參考：《第一軍醫(yī)大學(xué)》2006年碩士論文

【摘要】：細(xì)菌性腦膜炎是中樞神經(jīng)系統(tǒng)(central nerve system，CNS)最常見(jiàn)最嚴(yán)重的感染性疾病。盡管抗生素的廣泛使用，但細(xì)菌性腦膜炎的發(fā)病率仍有5％-40％，而幸存者神經(jīng)系統(tǒng)后遺癥的發(fā)生率高達(dá)30％。細(xì)菌性腦膜炎的高感染率和死亡率的主要原因之一是由于其發(fā)病機(jī)制還不清楚。 大腸桿菌(Escherichia coli E．coli)是導(dǎo)致新生兒腦膜炎最常見(jiàn)的革蘭氏陰性桿菌。已知大部分發(fā)生在新生兒中的細(xì)菌性腦膜炎是細(xì)菌血源性傳播的結(jié)果。進(jìn)入中樞神經(jīng)系統(tǒng)是細(xì)菌性腦膜炎發(fā)生的必要條件，但關(guān)于血循環(huán)中的大腸桿菌如何穿透由腦微血管內(nèi)皮細(xì)胞(brain microvascular endothelial cells，BMECs)等組成的血腦屏障(blood-brain barrier，BBB)，其機(jī)制仍待研究。目前在腦膜炎大腸桿菌感染組學(xué)的研究中，已經(jīng)確定部分基因與細(xì)菌穿透血腦屏障有關(guān)。通過(guò)TnphoA突變實(shí)驗(yàn)發(fā)現(xiàn)基因ibeA在細(xì)菌侵襲BMEC過(guò)程中發(fā)揮著重要作用，且為毒力株E．coli K1株所獨(dú)有的。包含著ibeA的基因島命名為GimA(a Genetic island of meningitic E．coli containing ibeA)。GimA全長(zhǎng)20.3kb，其GC含量(46.2％)與E．coli基因組的GC含量(50.8％)不同，含有4個(gè)操縱子，分別是ptnIPKC，cglDTEC，gcxKRCI和ibeRAT，共15個(gè)基因。該基因島是由美國(guó)南加州大學(xué)醫(yī)學(xué)院洛杉磯兒童醫(yī)院黃勝和教授發(fā)現(xiàn)并命名，通過(guò)信息學(xué)預(yù)測(cè)該基因島編碼10
[Abstract]:Bacterial meningitis is the most common and serious infectious disease in central nervous system (central nerve). Despite the widespread use of antibiotics, the incidence of bacterial meningitis is between 5 and 40, while the incidence of neurological sequelae in survivors is as high as 30. One of the main causes of high infection and mortality in bacterial meningitis is that its pathogenesis is unclear. Escherichia coli E. coli is the most common gram-negative bacilli causing neonatal meningitis. Bacterial meningitis, most of which is known to occur in newborns, is the result of bacterial blood transmission. Entering the central nervous system is a necessary condition for the occurrence of bacterial meningitis. However, the mechanism of how the Escherichia coli in blood circulation penetrates the blood-brain barrier composed of (brain microvascular endothelial cells and BMECs, etc., remains to be studied. Some genes have been identified as related to bacterial penetration of the blood-brain barrier in meningitis Escherichia coli infection studies. TnphoA mutation assay showed that the gene ibeA played an important role in the process of bacterial invasion of BMEC and was unique to the virulence strain E.coli K1. The gene island containing ibeA was named GimA (a genetic island of meningitic E.coli containing ibeA). GimA). Its GC content (46.2%) was different from that of E.coli genome (50.8%). There are four operons, ptnIPKCU cglDTECgcxKRCI and ibeRAT. there are 15 genes. The gene island was discovered and named by Professor Huang Shenghe, Los Angeles Children's Hospital, School of Medicine, University of Southern California, USA. It is predicted by informatics that the gene island encodes 10.
【學(xué)位授予單位】：第一軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R346

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