本文選題：血粘度增高動(dòng)物模型 + 腦缺氧��； 參考：《蘭州大學(xué)》2006年碩士論文

【摘要】： 目的：1．探討血粘度增高模型大鼠血液粘度與動(dòng)脈血氧含量的相關(guān)性。2．用模型動(dòng)物血清干預(yù)腦細(xì)胞，觀察細(xì)胞凋亡及細(xì)胞內(nèi)游離鈣離子([Ca~(2+)]i)濃度變化，探討模型動(dòng)物血清中存在的致腦細(xì)胞毒性因素。 方法：1．采用高分子右旋糖酐(dextran，DT)法建立血粘度增高大鼠模型，用血液粘度儀、血液分析儀檢測(cè)血常規(guī)及血液流變學(xué)指標(biāo)的變化。 2．用i-STAT臨床血液分析系統(tǒng)(EG7~+血?dú)馄?檢測(cè)造模大鼠頸內(nèi)動(dòng)、靜脈血氧飽和度及血氧分壓的變化。 3．以Ca~(2+)特異性熒光探針Flou-3／AM為熒光指示劑，采用不同時(shí)間造模大鼠血清干預(yù)培養(yǎng)的大鼠原代腦細(xì)胞，在激光共聚焦顯微鏡下分時(shí)間段實(shí)時(shí)動(dòng)態(tài)觀察[Ca~(2+)]i的變化。 4．以吖啶橙(acridine orange，AO)和碘化丙錠(propidium iodide，PI)為染色劑，在激光共聚焦顯微鏡下分時(shí)間段觀察經(jīng)造模5d大鼠血清培養(yǎng)的原代腦細(xì)胞凋亡和死亡情況。 結(jié)果：1．造模時(shí)間與全血粘度和血漿粘度呈正相關(guān)，與血液有形成份持續(xù)減少呈負(fù)相關(guān)。 2．造模大鼠全血粘度和血漿粘度與動(dòng)脈血氧含量(CaO_2)、靜脈血氧飽和度(SjvO_2)、靜脈血氧分壓(PvO_2)及靜脈血氧含量(CjvO_2)呈負(fù)相關(guān)，而與動(dòng)脈血氧飽和度(SaO_2)、動(dòng)脈血氧分壓(PaO_2)無相關(guān)性。 3．與正常對(duì)照比較，造模20min血清干預(yù)導(dǎo)致腦細(xì)胞[Ca~(2+)]i出現(xiàn)鈣波動(dòng)；造模5d血清干預(yù)導(dǎo)致腦細(xì)胞[Ca~(2+)]i出現(xiàn)持續(xù)性鈣超載。 4．造模5d血清干預(yù)腦細(xì)胞，，在30min內(nèi)出現(xiàn)凋亡和死亡細(xì)胞，且凋亡和死亡程度隨干預(yù)時(shí)間的延長(zhǎng)而加重。 結(jié)論：1．DT制作血粘度增高模型動(dòng)物方法簡(jiǎn)單易行，重復(fù)率高是理想的慢性缺氧缺血模型動(dòng)物。 2．CaO_2可作為DT制作血粘度增高模型動(dòng)物組織缺氧程度的評(píng)估指標(biāo)。 3．造模5d動(dòng)物血清中存在致腦細(xì)胞損傷因素，是何種成份起作用有待進(jìn)一步研究。
[Abstract]:Objective 1. To investigate the correlation between blood viscosity and arterial blood oxygen content in rats with increased blood viscosity. 2. To observe apoptosis and the concentration of intracellular free calcium ([Ca ~ (2)] I) in brain cells with model animal serum. To investigate the factors of brain cytotoxicity in the serum of model animals. Methods 1. The rat model of increased blood viscosity was established by dextran (DT) method, and blood viscometer was used. The changes of blood routine and hemorheological indexes were detected by blood analyzer. 2. The internal motion of the model rats was detected by i-STAT clinical blood analysis system (EG7 ~ XG). The changes of oxygen saturation and partial pressure of oxygen in vein. 3. The primary brain cells of rats were cultured with different time serum of model rats with Ca ~ (2) specific fluorescence probe Flou-3 / AM as fluorescent indicator. The changes of [Ca ~ (2)] _ I were observed in real time under laser confocal microscope. (4) acridine orange (acridine orangeo) and propidium iodide (Pi) were used as chromatin. The apoptosis and death of primary brain cells cultured in rat serum for 5 days were observed under laser confocal microscope. Results (1) the modeling time was positively correlated with the whole blood viscosity and plasma viscosity. The whole blood viscosity and plasma viscosity were negatively correlated with the arterial oxygen content (CaO2), the venous oxygen saturation (SjvO _ 2), the venous oxygen partial pressure (PvO _ 2) and the venous blood oxygen content (CjvO _ 2) in the model rats. However, there was no correlation with Sao _ 2 and Pao _ 2. 3. Compared with normal control, serum intervention of model 20min resulted in calcium fluctuation of [Ca ~ (2)] _ I in brain cells. The serum intervention for 5 days resulted in persistent calcium overload in brain cells. 4. After 5 days of model making, there were apoptosis and death cells in 30min, and the degree of apoptosis and death increased with the prolongation of intervention time. Conclusion 1. The method of making animal model with increased blood viscosity is simple and feasible. High repetition rate is an ideal chronic hypoxic ischemia model animal. 2. CaOs _ 2 can be used as an index to evaluate the hypoxic degree of model animal with increased blood viscosity of DT. 3. Modeling for 5 days There are brain cell injury factors in animal serum. What kind of composition works needs further study.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R363

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