本文選題：載脂蛋白E基因敲除小鼠 + 阿爾茨海默病��； 參考：《山東中醫(yī)藥大學》2005年碩士論文

【摘要】：目的:觀察載脂蛋白E基因敲除(ApoEKO)對小鼠行為學特征、血脂、軟腦膜微循環(huán)及腦組織病理形態(tài)學的影響。方法:取11、15～20、29～41周齡的ApoEKO小鼠,按周齡隨機分層分為青年模型組、成年模型組、老年模型組,取相同遺傳背景的11、29周齡正常C57BL/6J小鼠隨機分層分為青年對照組、老年對照組。分別對五組小鼠做跳臺試驗、自主運動試驗和轉(zhuǎn)棒耐疲勞試驗。將前四組小鼠分別稱重、麻醉,采用顱窗法測定軟腦膜微循環(huán)血流量,取下腔靜脈血測定血脂,后快速取腦組織,觀察其光鏡及透射電鏡下病理形態(tài)學變化。結(jié)果:老年ApoEKO小鼠比同齡C57BL/6J小鼠學習記憶能力明顯下降,隨著年齡的遞增,ApoEKO小鼠學習記憶能力明顯下降;ApoE基因敲除對小鼠自主運動無明顯影響;ApoEKO小鼠較同齡C57BL/6J小鼠中樞神經(jīng)系統(tǒng)和肢體協(xié)調(diào)能力明顯下降,且隨著年齡遞增,ApoEKO小鼠中樞神經(jīng)系統(tǒng)和肢體協(xié)調(diào)能力明顯下降;具有明顯的高脂血癥;軟腦膜微循環(huán)血流量顯著降低;腦組織病理形態(tài)學顯示軸突、樹突空泡變性,神經(jīng)原纖維纏結(jié),大量脂褐素,細胞外老年斑及微血管病變。結(jié)論:ApoEKO小鼠具有類似AD的行為學和病理學特征,具有高脂血癥,存在微循環(huán)障礙,可以作為一種接近遺傳因素損傷的AD的理想動物模型。
[Abstract]:Aim: to observe the effects of apolipoprotein E knockout (ApoEKOA) on behavior, lipid, pial microcirculation and pathomorphology of brain tissue in mice. Methods: ApoEKO mice were randomly divided into youth model group, adult model group and senile model group. The normal C57BL / 6J mice with the same genetic background were randomly divided into young control group and elderly control group. Five groups of mice were tested by bench test, independent exercise test and rotation bar fatigue test. The first four groups of mice were weighed and anesthetized respectively. The microcirculation blood flow of pia meninges was measured by cranial window method, blood lipids were measured by blood from inferior vena cava, and the brain tissue was removed quickly. The pathological changes of the mice were observed under light microscope and transmission electron microscope. Results: the learning and memory ability of the aged ApoEKO mice was significantly lower than that of C57BL / 6J mice. The ability of learning and memory of ApoEKO mice decreased with the increase of age. The ability of central nervous system and limb coordination of ApoEKO mice was significantly lower than that of C57BL / 6J mice. In addition, the central nervous system and limb coordination ability of ApoEKO mice decreased with age, hyperlipidemia, decreased blood flow of pial microcirculation, histopathological changes of axon and dendritic vacuolar degeneration in ApoEKO mice, and hyperlipidemia were observed in ApoEKO mice. Neurofibrillary tangles, lipofuscin, extracellular senile plaques and microvascular lesions. Conclusion the behavior and pathology of the mice with the disease were similar to AD, and the mice had hyperlipidemia and microcirculation disorder, which could be used as an ideal animal model for AD with genetic damage.
【學位授予單位】：山東中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R363;R361

【參考文獻】

相關期刊論文 前9條

1 孫永安;阿爾茨海默病患者BBB結(jié)構(gòu)的病理改變及其對AD發(fā)病的影響[J];國外醫(yī)學.神經(jīng)病學神經(jīng)外科學分冊;2004年02期

2 劉龍濤,張文高,鄭廣娟;載脂蛋白E基因敲除小鼠在動脈粥樣硬化研究中的應用[J];山東生物醫(yī)學工程;2002年04期

3 李則一,陳吉棣;有氧運動對ApoE基因缺陷小鼠動脈粥樣硬化斑塊形成的影響[J];中國運動醫(yī)學雜志;2000年03期

4 ;載脂蛋白E基因敲除小鼠心肌微動脈內(nèi)“微病灶”與外膜炎癥[J];中國病理生理雜志;2001年11期

5 錢采楙;第四屆全國老年性癡呆與相關疾病研討會暨老年性癡呆國際討論會紀要[J];中華老年醫(yī)學雜志;2004年08期

6 李晨虎,李春波,吳文源;腦血管病變與阿爾茨海默病關系的研究進展[J];中華精神科雜志;2004年02期

7 畢勝,張昱,吳江,王德生,盛雨辰,趙慶杰;載脂蛋白E基因型與阿爾茨海默病及其血清總膽固醇水平的關系[J];中國神經(jīng)免疫學和神經(jīng)病學雜志;2002年01期

8 屠亞軍,琦祖和,楊鵬;人突變apoE高脂血癥轉(zhuǎn)基因近交系小鼠Tg(0,apoE7,human)1模型的建立[J];中國醫(yī)學科學院學報;2000年03期

9 張文高,鄭廣娟,劉龍濤;中西藥物對載脂蛋白E基因敲除小鼠動脈粥樣硬化的干預作用[J];中西醫(yī)結(jié)合心腦血管病雜志;2003年08期

，

本文編號：2007867