發(fā)布時(shí)間：2018-06-10 10:02

  本文選題：成纖維細(xì)胞樣基質(zhì)細(xì)胞 + 不成熟樹突狀細(xì)胞　； 參考：《浙江大學(xué)》2006年碩士論文

【摘要】：樹突狀細(xì)胞(dendritic cell,DC)是機(jī)體內(nèi)功能最強(qiáng)大的抗原提呈細(xì)胞(Antigen-presenting cells,APC),是免疫反應(yīng)的啟動(dòng)者,因?yàn)樗哂歇?dú)特的刺激初始T細(xì)胞活化的能力而備受關(guān)注。但是隨著對(duì)DC研究的深入,DC細(xì)胞群體的異質(zhì)性越來(lái)越受到關(guān)注,DC不但可以啟動(dòng)免疫反應(yīng)而且也能負(fù)向調(diào)控免疫反應(yīng)。體內(nèi)不但存在啟動(dòng)免疫反應(yīng)的DC,同時(shí)也存在抑制免疫反應(yīng)的DC。目前對(duì)調(diào)節(jié)性DC(Regulatory DC)的研究很多都是在體外進(jìn)行的,如在原先的DC培養(yǎng)條件下加入各種免疫抑制性因子進(jìn)而將造血前體細(xì)胞、成熟或不成熟DC誘導(dǎo)成具有負(fù)向調(diào)控作用的調(diào)節(jié)性DC,但這種方法過于簡(jiǎn)化,不能反映體內(nèi)的真實(shí)情況,因而其誘導(dǎo)產(chǎn)生的DC可能就不一定代表體內(nèi)的真實(shí)細(xì)胞亞群。 機(jī)體是一個(gè)復(fù)雜的系統(tǒng),各個(gè)組分在各個(gè)層次都可能發(fā)生相互作用,因此,免疫系統(tǒng)肯定也在各個(gè)層面受到其他系統(tǒng)的影響而更好發(fā)揮作用。我們以前的研究工作表明脾臟微環(huán)境無(wú)論對(duì)成熟DC或造血前體細(xì)胞都有重要的影響,脾臟基質(zhì)細(xì)胞不但可以誘導(dǎo)造血前體細(xì)胞分化發(fā)育成為調(diào)節(jié)性DC,還可以誘導(dǎo)成熟DC進(jìn)一步增殖分化成調(diào)節(jié)性DC。脾臟作為二級(jí)淋巴器官是免疫系統(tǒng)的一個(gè)組成部分,其微環(huán)境對(duì)免疫細(xì)胞的影響已經(jīng)得到了一些闡明,那么其他實(shí)質(zhì)器官如肺臟、肝臟、腦會(huì)不會(huì)也對(duì)免疫細(xì)胞具有同樣的影響呢? 肺臟是機(jī)體最大的開放器官之一,每天肺臟都要接觸大量的外界抗原,因此免疫耐受的形成對(duì)肺臟尤為重要�；|(zhì)細(xì)胞(Stromal cells)作為器官的重要組分,除了其維持器官結(jié)構(gòu)的功能外,它對(duì)免疫系統(tǒng)會(huì)有什么影響嗎?有報(bào)道指出肺臟成纖維細(xì)胞與T細(xì)胞有比較緊密的聯(lián)系,如成纖維細(xì)胞通過PGE2抑制T細(xì)胞的活化后死亡(activation induced cell death),那么肺臟基質(zhì)細(xì)胞會(huì)對(duì)DC有什么影響嗎?體外長(zhǎng)期培養(yǎng)的細(xì)胞系可能有別于體內(nèi)基質(zhì)細(xì)胞組分,但仍然可以部分地模擬體內(nèi)的微環(huán)境,而且細(xì)胞系使用方便,因此鑒于以上的思考,我們建立了小鼠的肺臟成纖維細(xì)胞樣基質(zhì)細(xì)胞系,通過該細(xì)胞系與DC的共培養(yǎng)體系來(lái)研究共培養(yǎng)后的DC的各種變化。
[Abstract]:Dendritic cell (DCC) is the most powerful antigen-presenting cell (APCP) in the body. It is the promoter of immune response and has attracted much attention because of its unique ability to stimulate the activation of initial T cells. However, with the further study of DC, the heterogeneity of DC cell population has attracted more and more attention. DC can not only initiate the immune response, but also negatively regulate the immune response. There are not only DCs that initiate immune response, but also DCs that inhibit immune response. At present, many studies on regulatory DCM are carried out in vitro, such as adding various immunosuppressive factors in the original DC culture condition, which will result in hematopoietic progenitor cells. Mature or immature DCs are induced into regulatory DCs with negative regulatory effects, but this method is too simplified to reflect the real situation in the body. So the DC that it induces may not necessarily represent a real subgroup of cells in the body. The body is a complex system in which the components may interact at all levels, so, The immune system must also be better affected by other systems at all levels. Our previous work has shown that spleen microenvironment has important effects on both mature DC and hematopoietic progenitor cells. Splenic stromal cells can not only induce the differentiation and development of hematopoietic precursor cells into regulatory DCs, but also induce the further proliferation and differentiation of mature DCs into regulatory DCs. The spleen, as a secondary lymphoid organ, is an integral part of the immune system. The effect of its microenvironment on immune cells has been elucidated. Does the brain have the same effect on immune cells? lung is one of the largest open organs of the body. Every day, the lungs are exposed to a large number of external antigens, so the formation of immune tolerance is particularly important to the lungs. Stromal cells (Stromal cells) as an important component of the organ, in addition to its function to maintain the organ structure, how does it affect the immune system? It has been reported that lung fibroblasts are closely related to T cells. If fibroblasts inhibit the activation of T cells through PGE2, then how do lung stromal cells affect DC? Cell lines that have been cultured for a long time in vitro may be different from the components of stromal cells in vivo, but they can still partly mimic the microenvironment in vivo, and the cell lines are easy to use. Therefore, in view of the above considerations, A mouse lung fibroblast-like stromal cell line was established. The co-culture system of the cell line and DC was used to study the changes of DC after co-culture.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前2條

1 王文文;肺臟基質(zhì)細(xì)胞分泌的VEGF對(duì)樹突狀細(xì)胞分化發(fā)育的影響[D];泰山醫(yī)學(xué)院;2011年

2 祁雪萍;小鼠骨髓來(lái)源調(diào)節(jié)性樹突狀細(xì)胞的體外培養(yǎng)和鑒定[D];山西醫(yī)科大學(xué);2012年

，

本文編號(hào)：2002826