本文選題：神經(jīng)干細胞 + 分化��； 參考：《重慶醫(yī)科大學》2006年碩士論文

【摘要】： 研究背景與目的 神經(jīng)干細胞(neural stem cell, NSC)是近年來神經(jīng)科學領(lǐng)域的研究熱點。NSC是具有多向分化潛能和自我更新的能力細胞,NSC的增殖分化為神經(jīng)系統(tǒng)損傷和變性疾病的治療帶來了新希望。帕金森病(Parkinson disease,PD)是多巴胺能神經(jīng)元減少或者消失導致的神經(jīng)系統(tǒng)病變,增加DA能神經(jīng)元是治療該疾病的一種策略,通過對NSC進行體外誘導有望獲得足量的有效多巴胺能神經(jīng)元,為細胞移植治療研究打下基礎(chǔ)。本試驗?zāi)康耐ㄟ^用IL-1β、AA、IL-1β聯(lián)合AA誘導NSC分化生成多巴胺能神經(jīng)元,為深入研究NSC體外分化條件,探討誘導分化機制,提高DA能神經(jīng)元分化效能奠定細胞學基礎(chǔ)。 方法 分離培養(yǎng)新生(1日齡)SD大鼠中腦神經(jīng)干細胞,通過傳代培養(yǎng)擴增來檢測自我更新能力;免疫學方法檢測NSC特異性標志nestin蛋白表達和分化成為神經(jīng)元、神經(jīng)膠質(zhì)細胞能力。對NSC克隆進行IL-1β、AA、IL-1β聯(lián)合AA(抗壞血酸)誘導分化,通過免疫細胞學方法檢測多巴胺能神經(jīng)元標志物酪氨酸羥化酶(tyrosine tydroxylase,TH)表達。 結(jié)果
[Abstract]:Research background and purpose Neural stem cell neural stem cell, NSC) is a research hotspot in the field of neuroscience in recent years. The proliferation and differentiation of neural stem cell neural stem cell, NSC), which has the potential of multiple differentiation and self-renewal, has brought new hope to the treatment of nervous system injury and degeneration. Parkinson disease (PD) is a neuropathy caused by the reduction or disappearance of dopaminergic neurons. Increasing DA neurons is a strategy for the treatment of the disease. It is hoped that sufficient effective dopaminergic neurons can be obtained by inducing NSC in vitro. To lay a foundation for the study of cell transplantation therapy. Objective to induce the differentiation of NSC into dopaminergic neurons by IL-1 尾 -AA-IL-1 尾 combined with AA, and to establish the cytological basis for further studying the differentiation conditions of NSC in vitro, exploring the mechanism of differentiation and enhancing the differentiation efficiency of DA neurons. Method The mesencephalic neural stem cells (NSCs) of 1-day-old SD rats were isolated and cultured, and the ability of self-renewal was detected by subculture and amplification. The expression and differentiation of nestin protein, a specific marker of NSC, into neurons and glial cells were detected by immunological methods. The NSC clone was induced by IL-1 尾 -AAAA-IL-1 尾 and ascorbic acid (ascorbic acid). The expression of tyrosine tydroxylasethase (THH), a dopaminergic neuron marker, was detected by immunocytology. Result
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R329

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本文編號：1982417