本文選題：甲型肝炎病毒 + 基因組��； 參考：《吉林大學(xué)》2006年碩士論文

【摘要】： 本論文在成功地進(jìn)行了毒株的分離,適應(yīng)性培養(yǎng)以及鑒定之后,從傳代的細(xì)胞中提取出病毒BL-08的基因組RNA,再采用逆轉(zhuǎn)錄-聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)對甲肝病毒BL-08進(jìn)行擴(kuò)增、克隆、測序得基因組序列nt25-7476。再將HAV BL-08株的基因序列與其它株型進(jìn)行比較,得到了以下的結(jié)果:全長核苷酸序列同源性與MBB株最高,為98.8%;與L-A-1株次之為98.0%,與HM175野毒株的同源性為95.0%。而在氨基酸同源性方面卻顯示出相反的結(jié)果,MBB株和HM175株與BL-08序列的氨基酸同源性分別為98.4%和98.5%。 另外,本論文還著重對甲肝病毒BL-08株的5’NTR區(qū)進(jìn)行研究。pHAV/7質(zhì)粒是由PH.D.Suzanne Emerson提供的,以pEGM載體為骨架,帶有HAV HM175株全基因組序列的質(zhì)粒,全長10358bp。本實(shí)驗(yàn)在原有的pHAV/7的基礎(chǔ)上,將有內(nèi)部核糖體結(jié)合位點(diǎn)(IRES)的5’NTR區(qū)域與BL-08株做相應(yīng)位置的置換,然后將其和pHAV/7進(jìn)行比較,從而分析單獨(dú)的5’NTR區(qū)對于HAV病毒生長周期的影響。
[Abstract]:After successful isolation, adaptive culture and identification of the virus strain, the genomic RNAs of virus BL-08 were extracted from the passage cells and then amplified and cloned by reverse transcription-polymerase chain reaction (RT-PCR). The genomic sequence nt25-7476 was sequenced. By comparing the gene sequence of HAV BL-08 strain with other strains, the following results were obtained: the homology of full-length nucleotide sequence with MBB strain was 98.8, that with L-A-1 strain was 98.0 and that with HM175 field strain was 95.0. The results of amino acid homology showed that the amino acid homology between MBB strain and HM175 strain was 98.4% and 98.5% with BL-08 sequence, respectively. In addition, the 5'NTR region of hepatitis A virus (BL-08) strain was studied in this paper. The plasmid pHAV / 7 was provided by PH.D.Suzanne Emerson. The plasmid containing the whole genome sequence of HAV HM175 strain was pEGM vector and the whole length of plasmid was 10358bp. On the basis of the original pHAV/7, the 5'NTR region with internal ribosomal binding site (IRES) was replaced with the BL-08 strain, and then compared with pHAV/7. The effect of single 5'NTR region on the growth cycle of HAV virus was analyzed.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R373.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 周翔,譚德勇,賴建華,昝瑞光;甲肝病毒減毒株(H_2)RNA的全長RT-PCR擴(kuò)增[J];生物化學(xué)與生物物理進(jìn)展;1998年05期

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本文編號：1981853