本文選題：脫氧核酶 + 流感病毒�。� 參考：《重慶醫(yī)科大學(xué)》2006年碩士論文

【摘要】： 背景與目的 流行性感冒病毒(Influenza virus,IFV),簡稱流感病毒,是引起流行性感冒的病原體。流感長期以來一直嚴(yán)重威脅著人類的健康,近幾年研究發(fā)現(xiàn),禽流感等可以跨越種屬差異,由禽類傳染人類,給人類的生命、財產(chǎn)安全都帶來更大的威脅,引起了全球各界的高度重視。因此,開展流感病毒的研究和防治具有重要的科學(xué)意義和實(shí)際應(yīng)用價值。 疫苗接種是目前預(yù)防病毒性疾病的有效措施。但由于流感病毒表面抗原容易發(fā)生變異,致使疫苗預(yù)防流感效果欠佳。現(xiàn)在所應(yīng)用的抗流感藥物主要有金剛烷胺類和神經(jīng)氨酸酶(Neuraminidase,NA)抑制劑類兩類藥物,由于它們的毒副作用,限制了臨床中的應(yīng)用。因此開發(fā)更安全、有效的新型的抗流感病毒藥物已勢在必行。 對流感病毒復(fù)制和轉(zhuǎn)錄起關(guān)鍵作用的RNA聚合酶(由PB1, PB2和PA三個亞基組成),在流感病毒的生活周期中發(fā)揮著重要作用,其中聚合酶堿性蛋白1(PB1)亞基具有RNA聚合酶活性,既參與mRNA轉(zhuǎn)錄又參與病毒RNA(vRNA)的復(fù)制,并催化新合成的RNA鏈延伸,變異性相對較小�？梢宰鳛橐环N潛在的抗流感病毒藥物的作用靶點(diǎn),國外已有針對PB1-mRNA反義寡聚核苷酸(ASO)用于抑制病毒復(fù)制的研究,其在無細(xì)胞系統(tǒng)具有較好的切割效果。
[Abstract]:Background and purpose Influenza virus Influenza virus, or influenza virus, is the causative agent of influenza. Influenza has been a serious threat to human health for a long time. In recent years, studies have found that bird flu and so on can cross species differences, infect humans by birds, and pose a greater threat to human life and property security. It has aroused great attention from all walks of life around the world. Therefore, the research and prevention of influenza virus has important scientific significance and practical application value. Vaccination is an effective measure to prevent viral diseases. However, the surface antigen of influenza virus is easy to mutate, which results in poor effect of vaccine against influenza. The main antiinfluenza drugs used are amantadine and neuraminidase NAA inhibitors, which limit the clinical application due to their side effects. Therefore, the development of safer and more effective new anti-influenza drugs is imperative. The RNA polymerase (composed of PB1, PB2 and PA), which plays a key role in influenza virus replication and transcription, plays an important role in the life cycle of influenza virus. The polymerase basic protein 1 (PB1) subunit has the activity of RNA polymerase. It is involved in both mRNA transcription and viral RNA RNA replication, and catalyzes the newly synthesized RNA strand extension. The variability is relatively small. PB1-mRNA antisense oligodeoxynucleotides (ASO) have been used to inhibit viral replication and have good cleavage effect in acellular system.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R373

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 徐玉鳳;劉家國;趙彪;王德云;范云鵬;胡元亮;徐樹培;廖敬宜;;板藍(lán)根生物堿酸性部位對新城疫病毒吸附和釋放的影響[J];南京農(nóng)業(yè)大學(xué)學(xué)報;2010年06期

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本文編號：1968563