本文選題：大鼠 + 鉀通道�。� 參考：《華中科技大學》2007年碩士論文

【摘要】： 一、ATP敏感性鉀通道對大鼠前脂肪細胞增殖分化影響 目的:探討ATP敏感性鉀通道在大鼠前脂肪細胞增殖分化中作用。 方法:逆轉錄PCR檢測大鼠腦組織、脂肪組織、前脂肪細胞和誘導5天獲得的脂肪細胞中ATP敏感性鉀通道磺脲類受體mRNA (SURmRNA)亞型,實時定量PCR檢測前脂肪細胞和誘導5天獲得的脂肪細胞中SURmRNA表達;ATP敏感性鉀通道阻滯劑格列本脲和激動劑二氮嗪作用于前脂肪細胞,實時定量PCR檢測SURmRNA表達,MTT檢測前脂肪細胞增殖,流式細胞儀檢測細胞周期分布;油紅O染色法檢測細胞內脂質含量,Image-Pro plus5.0軟件測量細胞直徑。 結果:大鼠腦組織中有SUR1mRNA、SUR2mRNA表達,大鼠脂肪組織、前脂肪細胞和誘導5天獲得的脂肪細胞中均有SUR2mRNA表達,而無SUR1mRNA表達,且脂肪細胞中SUR2mRNA表達明顯高于前脂肪細胞;格列本脲抑制前脂肪細胞SUR2mRNA表達,呈劑量依賴性地促進前脂肪細胞增殖,增加G2/M+S期細胞百分率,增加細胞脂質含量,使脂肪細胞直徑增大;二氮嗪在這些方面的作用與格列本脲相反。 結論:ATP敏感性鉀通道在前脂肪細胞增殖分化中可能起調節(jié)作用。 二、ATP敏感性鉀通道調節(jié)大鼠前脂肪細胞增殖分化機制研究 目的:探索ATP敏感性鉀通道調節(jié)大鼠前脂肪細胞增殖分化機制。 方法:ATP敏感性鉀通道特異性激動劑(二氮嗪)、阻滯劑(格列本脲)作用于前脂肪細胞誘導分化5天后,逆轉錄PCR檢測瘦素mRNA表達及過氧化物酶體增殖物激活受體γ(PPARγ)mRNA表達。二氮嗪、格列本脲作用于前脂肪細胞一天,Western blot檢測其細胞內P21、P27蛋白表達。 結果:前脂肪細胞分化過程中,二氮嗪組瘦素mRNA表達明顯增加(p0.05),格列本脲組瘦素mRNA表達明顯降低(p0.05);格列本脲組PPARγmRNA表達增加(p0.05),而二氮嗪組PPARγmRNA表達減少(p0.05)。二氮嗪作用于前脂肪細胞,使細胞內P21、P27表達增高(p0.05),而格列本脲作用于前脂肪細胞后,細胞內P21、P27表達降低(p0.05)。 結論:ATP敏感性鉀通道調節(jié)大鼠前脂肪細胞增殖分化,可能與瘦素mRNA、P21、P27蛋白表達上調,PPARγmRNA表達下調有關
[Abstract]:Effects of ATP-sensitive potassium channels on proliferation and differentiation of rat preadipocytes Aim: to investigate the role of ATP sensitive potassium channel in the proliferation and differentiation of rat preadipocytes. Methods: reverse transcription PCR was used to detect the subtypes of ATP sensitive potassium channel sulfonylurea receptor (mRNA) in rat brain, adipocytes, preadipocytes and adipocytes induced for 5 days. Real-time quantitative PCR was used to detect SURmRNA expression in preadipocytes and adipocytes obtained after 5 days of induction. Glibenclamide, an ATP-sensitive potassium channel blocker, and diazazine, a agonist, acted on preadipocytes. The expression of SURmRNA was detected by real-time quantitative PCR, the proliferation of adipocytes was detected by flow cytometry, the lipid content in cells was detected by oil red O staining and the diameter of adipocytes was measured by Image-Pro plus5.0 software. Results: the expression of SUR1mRNA-SUR2 mRNA was found in rat brain tissue. The expression of SUR2mRNA was found in adipose tissue, preadipocytes and adipocytes induced for 5 days, but no SUR1mRNA expression was found in adipocytes. The expression of SUR2mRNA in adipocytes was significantly higher than that in preadipocytes. Glibenclamide inhibited the expression of SUR2mRNA in preadipocytes, promoted the proliferation of preadipocytes in a dose-dependent manner, increased the percentage of G2 / MS phase cells, increased the lipid content and increased the diameter of adipocytes. Diazazine has the opposite effect on these aspects as glibenclamide. Conclusion the cell proliferation and differentiation of preadipocytes may be regulated by K 2 + channel. Mechanism of ATP-sensitive potassium channels regulating proliferation and differentiation of rat preadipocytes Aim: to explore the mechanism of ATP sensitive potassium channel regulating the proliferation and differentiation of preadipocytes in rats. Methods expression of leptin mRNA and peroxisome proliferator activated receptor 緯 -PPAR 緯 -PPAR 緯 mRNA were detected by reverse transcriptase PCR (RT PCR) after differentiation of preadipocytes was induced by diazosin and glibenclamide (glibenclamide). Preadipocytes were treated with diazazine and glibenclamide for one day. Western blot was used to detect the expression of P21 P27 protein in preadipocytes. Results: during the differentiation of preadipocytes, the expression of leptin mRNA increased significantly in diazazine group, the leptin mRNA expression in glibenclamide group decreased significantly, and the expression of PPAR 緯 mRNA increased in glibenclamide group, while PPAR 緯 mRNA expression decreased in diazazine group. After treated with diazoxide, the expression of P21 P27 in preadipocytes increased, while glibenclamide decreased the expression of P21 P27 in preadipocytes. Conclusion the proliferation and differentiation of preadipocytes regulated by the potassium channel of 1: ATP-sensitive may be related to the up-regulation of the expression of leptin mRNA-P21p27 protein and down-regulation of the expression of PPAR- 緯 mRNA in rat preadipocytes.
【學位授予單位】：華中科技大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R329

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本文編號：1955459