本文選題：乳房 + 乳房下皺襞　； 參考：《河北醫(yī)科大學(xué)》2005年碩士論文

【摘要】：目的:通過對乳房下皺襞相關(guān)組織結(jié)構(gòu)的大體解剖、組織學(xué)及臨床病例的研究,進(jìn)一步明確乳房下皺襞的組織解剖結(jié)構(gòu)和形成原理,為乳腺外科學(xué)及乳房整形再造術(shù)提供解剖學(xué)依據(jù)。 方法:1. 標(biāo)本分組:采用新鮮成年女性尸體10 具(36-69 歲),乳房20 只。每只乳房在4 個不同的部位分別取標(biāo)本。第一組:乳房上緣;第二組:乳暈上緣;第三組:乳房下皺襞;第四組:乳房下胸壁,距乳房下皺襞最低點(diǎn)4cm。每組均位于經(jīng)乳房下皺襞最低點(diǎn)做垂直于鎖骨中點(diǎn)的連線上。2.試驗(yàn)前主要試劑的準(zhǔn)備:①氨性銀染色液的配制。②維多利亞藍(lán)染色液的配制。③麗春紅染色液的配制。3. 取材:尸體標(biāo)本平臥于解剖臺上,切口均為沿皮紋方向,平行于乳房下皺襞切面,垂直于皮膚表面切下。光鏡標(biāo)本切成1cm~3大小的組織塊,放入10%中性甲醛液中固定24 小時以上,在梯度濃度的乙醇中脫水,石蠟包埋,制做光鏡切片;電鏡標(biāo)本切成3-5mm~3 的小塊,立即置入預(yù)冷(4℃)2.5%戊二醛中固定24 小時以上,制做電鏡標(biāo)本。4. 觀察方法:①大體解剖:尸體標(biāo)本平臥于解剖臺上,以解剖刀逐層分離皮膚和皮下組織以及腺體組織,觀察其纖維走行,特別是乳房下皺襞區(qū)域的纖維結(jié)構(gòu),觀察其是否有如文獻(xiàn)所述的橫行或縱行的韌帶結(jié)構(gòu)。②在光學(xué)顯微鏡下分別以低倍視野和高倍視野觀察切片,注意觀察纖維組織的形態(tài)特征,并分別在
[Abstract]:Objective: through the study of gross anatomy, histology and clinical cases of submammary folds, to further clarify the anatomical structure and formation principle of submammary folds. To provide anatomical basis for breast surgery and breast plastic reconstruction. Method 1: 1. The specimens were divided into 10 fresh adult female cadavers aged 36-69 and 20 breasts. Each breast was sampled in 4 different parts. Group 1: superior edge of breast; group 2: superior edge of areola; group 3: submammary fold; group 4: submammary chest wall, 4cm from the lowest point of submammary fold. Each group was located on a line perpendicular to the middle of the clavicle through the lowest point of the submammary fold. Preparation of main Reagents before experiment. Preparation of ammonia Silver dyeing solution .2 preparation of Victorian Blue dyeing solution. Material: the cadaveric specimen is lying on the dissection table, the incision is along the direction of the skin stripe, parallel to the cut surface of the submammary fold, perpendicular to the skin surface. Light microscope specimens were cut into 1cm~3 size tissue blocks, fixed in 10% neutral formaldehyde solution for more than 24 hours, dehydrated in gradient concentration of ethanol, embedded in paraffin to make light microscope sections. Electron microscope specimens were cut into small pieces of 3-5mm~3. The electron microscope specimen was prepared by immobilization of 2.5% glutaraldehyde at 4 鈩，

本文編號：1949634