本文選題：N-RAS基因 + 二級結(jié)構(gòu)��； 參考：《第一軍醫(yī)大學(xué)》2005年碩士論文

【摘要】：RAS基因是最容易被激活的人類原癌基因之一,在一個龐大的信號傳遞網(wǎng)絡(luò)中起著著分子開關(guān)的作用,這個網(wǎng)絡(luò)主要控制細(xì)胞的增殖和分化。RAS基因突變后被激活,成為具有致癌作用的癌基因,編碼具有持續(xù)活性的蛋白,持續(xù)激活下游通路,可使細(xì)胞發(fā)生不依賴于生長因子的生存和增殖,導(dǎo)致腫瘤的形成。 就目前所知,點突變是RAS基因激活的主要方式。在已發(fā)現(xiàn)多種不同的腫瘤(大部分為上皮細(xì)胞衍生而來)中,物理或化學(xué)致癌因素均可引起RAS基因突變。由于其在腫瘤發(fā)生發(fā)展中的重要作用,RAS基因已成為基礎(chǔ)及臨床研究的重要對象。K-RAS突變常見于胰、結(jié)腸及肺部腺癌,泌尿系統(tǒng)腫瘤則以H-RAS突變?yōu)橹?而在急性淋巴細(xì)胞白血病,慢性淋巴細(xì)胞白血病等血液系統(tǒng)腫瘤中以N-RAS突變?yōu)橹鳌?研究RAS的信號轉(zhuǎn)導(dǎo)通路有助于尋找新的抗癌藥物治療作用靶點。但是,越來越多的證據(jù)顯示,信號轉(zhuǎn)導(dǎo)通路中,各信號通路是個復(fù)雜的網(wǎng)絡(luò)結(jié)構(gòu),各通路是互相影響、互相交叉的。由于這種復(fù)雜的網(wǎng)絡(luò)結(jié)構(gòu)的存在,對RAS基因在各個控制細(xì)胞表型的信號通路中的位置與作用的研究存在著一定的困難。 RNA干擾(RNA interference,RNAi)是近幾年內(nèi)迅速發(fā)展起來的基因沉默方法,用于特異性去除或降低某個或某些基因在細(xì)胞或機(jī)體中的表達(dá),研究正�；虻慕Y(jié)構(gòu)、功能、信號傳導(dǎo)通路機(jī)制。研究發(fā)現(xiàn),體外合成的21～23nt的siRNA
[Abstract]:The RAS gene is one of the most easily activated human proto-oncogenes, acting as a molecular switch in a large signaling network that controls cell proliferation and differentiation. Ras gene mutations are then activated. As a carcinogenic oncogene, which encodes a protein with sustained activity and continuously activates downstream pathways, cell survival and proliferation are independent of growth factors, leading to tumor formation. As far as known, point mutation is the main mode of RAS gene activation. In many different tumors, most of which are derived from epithelial cells, both physical and chemical carcinogenic factors can cause mutations in the RAS gene. Because of its important role in tumorigenesis and development, Ras gene has become an important object of basic and clinical research. K-RAS mutation is common in pancreatic, colon and lung adenocarcinoma, and urinary system tumors are mainly H-RAS mutation, but in acute lymphoblastic leukemia. N-RAS mutation was dominant in hematological tumors such as chronic lymphoblastic leukemia. To study the signal transduction pathway of RAS is helpful to find new targets of anticancer drug therapy. However, more and more evidence shows that, in signal transduction pathways, each signal pathway is a complex network structure, and each pathway interacts with each other. Due to the existence of this complex network structure, it is difficult to study the position and role of RAS gene in various signal pathways that control cell phenotype. RNA interference RNAi is a rapidly developed gene silencing method in recent years. It is used to specifically remove or decrease the expression of one or some genes in cells or organisms, and to study the structure, function and signal transduction pathway mechanism of normal genes. It was found that siRNA of 21~23nt was synthesized in vitro.
【學(xué)位授予單位】：第一軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R346

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 王文濤;豬Cst B基因的克隆、多態(tài)性分析及表達(dá)規(guī)律的研究[D];東北農(nóng)業(yè)大學(xué);2007年

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本文編號：1943261