本文選題：側(cè)腦室 + 腹外側(cè)視前區(qū)　； 參考：《蘭州大學(xué)》2006年碩士論文

【摘要】：目的：應(yīng)用情緒行為活動測定、腦立體定位、側(cè)腦室插管、微量注射、多導(dǎo)睡眠描記和免疫組織化學(xué)等方法，觀察P物質(zhì)和其拮抗劑(N-acetyl-L-tryptophan 3,5-bis(trifluoromethyl)-benzyl ester，NALTBE)對大鼠睡眠-覺醒周期及其情緒活動的影響。 方法：80只成年雄性Sprague-Dawley大鼠隨機分為睡眠記錄組和情緒行為組。睡眠記錄組大鼠經(jīng)水合氯醛(350mg／kg)腹腔注射麻醉后，將頭部固定于SN-2型腦立體定位儀上，暴露顱骨，雙氧水清潔顱骨表面，在冠狀縫前2mm及人字縫前1mm與顱骨中線兩側(cè)旁開1mm交叉點處分別安裝EEG電極，，深度以穿透顱骨接觸到硬腦膜為度，用于記錄皮層腦電活動；在雙側(cè)頸肌內(nèi)插入EMG電極用于記錄肌電活動。EEG、EMG記錄電極引導(dǎo)線均連接到一個微型插座上。外徑為23gauge(0.6mm，OD)的不銹鋼引導(dǎo)管按Paxinos和Watson大鼠腦立體定位圖譜插入：右側(cè)腦室(AP：-0.8mm，R：+1.5mm、H：-4mm)，引導(dǎo)管頂端距側(cè)腦室1mm，供側(cè)腦室內(nèi)微量注射藥物用。所有電極和引導(dǎo)管均用牙科水泥固定于大鼠的顱骨上。情緒行為組大鼠只埋入引導(dǎo)管，不安裝電極。 手術(shù)后，大鼠在記錄艙內(nèi)飼養(yǎng)1周以恢復(fù)和適應(yīng)環(huán)境。睡眠記錄組進一步分為：PBS注射組(PBS，0.1M，PH=7.4，n=8)；SP 1 pmol組(n=8)；SP 10 pmol組(n=8)；SP 100 pmol組(n=8)；Anti-sP組(N-acetyl-L-tryptophan
[Abstract]:Objective: to investigate the effects of mood and behavioral activity determination, stereotactic localization, lateral ventricular intubation, microinjection, polysomnography and immunohistochemistry. To investigate the effects of substance P and its antagonist N-acetyl-L-tryptophan (N-acetyl-L-tryptophan) on sleep-wake cycle and emotional activity in rats. Methods 80 adult male Sprague-Dawley rats were randomly divided into sleep recording group and emotional behavior group. Rats in the sleep recording group were anesthetized by intraperitoneal injection of chloral hydrate (350mg / kg). The head was fixed on the SN-2 brain stereotactic locator to expose the skull, and hydrogen peroxide was used to clean the surface of the skull. EEG electrodes were installed at 2mm before coronal suture and 1mm crossing point between 1mm and midline of skull before herringbone suture. The depth of EEG electrode was used to record cortical electroencephalogram (EEG) activity by penetrating the skull into the dura mater. The EMG electrode was inserted into the bilateral cervical muscles to record the electromyoelectric activity. The EEGG EMG recording electrode guide wire was connected to a micro socket. The stainless steel guide tube with external diameter of 23gauge.6mm OD) was inserted according to the stereotaxic map of Paxinos and Watson rats. The right ventricle (AP: 0.8mm) was inserted as follows: the right ventricle (AP: 0.8mm), the right ventricle (1.5 mm), the apical lateral ventricle (1mm), and the donor ventricle for microinjection. All electrodes and guide tubes were fixed to the skull of rats with dental cement. In the emotional behavior group, only the guide tube was embedded, and the electrodes were not installed. After operation, rats were kept in the recording chamber for 1 week to recover and adapt to the environment. The sleep record group was further divided into two groups: PBS0.1 pmol 7.4MnPBS, SP1 pmol, SP10 pmol, SP100 pmol, N-acetyl-L-tryptophan (N-acetyl-L-tryptophan).
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R33

【參考文獻】

相關(guān)期刊論文 前4條

1 李紅勝，趙志奇;P物質(zhì)的分子神經(jīng)生物學(xué)研究進展[J];生理科學(xué)進展;1994年01期

2 鐘明奎,張瑾,朱國慶,張景行,趙樂章,章功良;損毀和興奮基底外側(cè)杏仁核對大鼠行為活動的影響[J];安徽醫(yī)科大學(xué)學(xué)報;2003年01期

3 朱國慶,鐘明奎,張景行,趙樂章,章功良,高雋;阿片機制參與基底外側(cè)杏仁核的睡眠調(diào)節(jié)作用(英文)[J];中國神經(jīng)科學(xué)雜志;1999年04期

4 張景行;探討深慢波睡眠機制的重要意義[J];中國中醫(yī)基礎(chǔ)醫(yī)學(xué)雜志;2001年08期

本文編號：1942490