本文選題：藍(lán)斑神經(jīng)元 + 腦片��； 參考：《中國(guó)科學(xué)院研究生院（上海生命科學(xué)研究院）》2007年博士論文

【摘要】： 藍(lán)斑(Locus Coeruleus, LC)核團(tuán)主要含有去甲腎上腺素(NA)神經(jīng)元并且投射到中樞神經(jīng)系統(tǒng)的大部分區(qū)域。然而,有關(guān)NA如何從藍(lán)斑神經(jīng)元釋放卻知之甚少。本學(xué)位工作聯(lián)合電化學(xué)記錄和膜片鉗測(cè)量技術(shù)發(fā)現(xiàn):去極化刺激藍(lán)斑神經(jīng)元可以記錄到被誘發(fā)的尖峰(spike)信號(hào)(Iamp),每個(gè)尖峰代表一個(gè)量子化的遞質(zhì)釋放;去極化脈沖誘發(fā)的量子化NA分泌信號(hào)的平均潛伏期是1870ms;而藍(lán)斑神經(jīng)元谷氨酸介導(dǎo)的興奮性突觸后電位的平均潛伏期只有1.6ms;這些量子化安培信號(hào)的釋放是依賴鈣離子的,去除細(xì)胞外的鈣離子可以阻斷釋放; TTX不能阻斷去極化脈沖誘發(fā)的藍(lán)斑神經(jīng)元的遞質(zhì)釋放,在急性游離的藍(lán)斑神經(jīng)元胞體部位可以記錄到去極化刺激誘發(fā)的尖峰信號(hào)和膜電容的增加,說(shuō)明有一部分遞質(zhì)釋放來(lái)自胞體;相對(duì)于谷氨酸能的快速EPSC,腦片上藍(lán)斑神經(jīng)元的兒茶酚胺的分泌更易受到動(dòng)作電位頻率的調(diào)節(jié)(0.5-50 Hz),神經(jīng)元在自發(fā)放電狀態(tài)下,胞體釋放量很少,而在受到刺激后,放電頻率增加時(shí),胞體釋放也增多;藍(lán)斑核團(tuán)內(nèi)神經(jīng)元興奮性增強(qiáng)時(shí),使胞體樹(shù)突區(qū)域的NA釋放,繼而抑制胞體本身的放電和NA的釋放,這種作用與胞體上α2腎上腺素能受體有關(guān)。 綜上所述,與經(jīng)典的突觸遞質(zhì)釋放相比,藍(lán)斑胞體部位的NA的量子化釋放具有潛伏期長(zhǎng),對(duì)動(dòng)作電位模式高度敏感的特點(diǎn)。
[Abstract]:The nucleus of Locus Coeruleus (LC) mainly contains norepinephrine (NAN) neurons and projects to most areas of the central nervous system (CNS). However, little is known about how na is released from locus coeruleus neurons. In combination with electrochemical recording and patch clamp measurement, it was found that depolarization-stimulated locus coeruleus neurons could record evoked spike-spike signals, each peak representing a quantized release of a transmitter; The average latency of quantized na secreting signals induced by depolarization pulses was 1870 Ms, while that of glutamate mediated excitatory postsynaptic potentials in locus coeruleus neurons was only 1.6 Ms. Removal of extracellular calcium ions could block release, TTX could not block the release of neurotransmitters from locus coeruleus neurons induced by depolarization pulse. The spike signal and the increase of membrane capacitance induced by depolarization stimulation could be recorded in the acute free locus coeruleus neurons, indicating that some of the transmitters were released from the cell body. The catecholamine secretion of locus coeruleus neurons was more likely to be regulated by the frequency of action potential 0.5-50 Hz than that of glutaminergic EPSC.When the neurons were spontaneously discharges, the release of catecholamine from the neurons was very small, but after stimulation, the secretion of catecholamine was more likely to be regulated by the action potential frequency. When the frequency of discharge increased, the release of the cell body increased, and when the excitability of neurons in the locus coeruleus increased, the na release in the dendritic region of the cell body was released, which in turn inhibited the discharge of the cell body itself and the release of na. This action is related to 偽 2 adrenoceptor on the cell body. In conclusion, compared with the classical synaptic transmitter release, the quantized release of na in the locus of locus coeruleus has long latency and is highly sensitive to the action potential pattern.
【學(xué)位授予單位】：中國(guó)科學(xué)院研究生院（上海生命科學(xué)研究院）
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2007
【分類號(hào)】：R338

【共引文獻(xiàn)】

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本文編號(hào)：1911652