本文選題：三重腦震蕩 + 腦神經(jīng)元��； 參考：《昆明醫(yī)學(xué)院》2006年碩士論文

【摘要】：目的：建立三重腦震蕩(multidle cerebral concussion，MCC)大鼠模型，觀察MCC大鼠大腦皮層、背側(cè)海馬、齒狀回和腦干網(wǎng)狀結(jié)構(gòu)神經(jīng)元的組織學(xué)變化及5羥色胺能神經(jīng)元、神經(jīng)纖維變化的規(guī)律，探討與MCC大鼠認(rèn)知行為障礙相關(guān)腦區(qū)的神經(jīng)元和5羥色胺能神經(jīng)變化，，以了解三重腦震蕩鼠認(rèn)知行為障礙的神經(jīng)生物學(xué)基礎(chǔ)。方法：應(yīng)用金屬單擺打擊裝置復(fù)制大鼠MCC模型48只，大鼠隨機分為1天、2天、4天、8天、16天和24天6個三重腦震蕩組(n=8)。正常對照組(n=8)。用甲苯紫尼氏染色顯示神經(jīng)元，并用光鏡對大鼠上述腦區(qū)的固縮變性神經(jīng)元進行計數(shù)；用TPH、5—HT抗體免疫組織化學(xué)的方法檢測大鼠腦干中縫5羥色胺能神經(jīng)元遞質(zhì)(5—HT)及5—HT合成過程中的限速酶——色氨酸羥化酶(TPH)的表達；用5—HT抗體免疫組化的方法檢測大腦皮質(zhì)、中隔區(qū)、伏隔核內(nèi)側(cè)、丘腦、杏仁體及海馬5羥色胺神經(jīng)纖維的變化。結(jié)果：(1)MCC后腦神經(jīng)元組織化學(xué)研究結(jié)果：與對照組相比，MCC后1天組大腦前額葉皮質(zhì)、頂葉皮質(zhì)和梨狀皮質(zhì)固縮變性神經(jīng)元丌始增加，2天組達高峰(P＜0.05)，4天、8天組有所減少，16天組又有所回升，24天組基本恢復(fù)正常。顳葉皮質(zhì)固縮變性神經(jīng)元在傷后2天達到高峰，4天有所減弱，8天又有回升并接近高峰值，24天組基本恢復(fù)正常。海馬CA1～CA4和上、下齒狀回固縮變性神經(jīng)元也在傷后1天開始增多，海馬CA1～CA4及上齒狀回變性神經(jīng)元數(shù)2天達頂峰，下齒狀回變性神經(jīng)元數(shù)16天達高峰，至24天與對照組比較無顯著性差異。在腦干網(wǎng)狀結(jié)構(gòu)腦橋核平面，除16天、24天外，其他各組與對照組比較均有差異(P＜0.05)。病變以傷后2天、4天、8天最重(P＜0.01)，16天有所減輕，24天組基本恢復(fù)正常。橄欖核平面病變以傷后2天、4天、8天最重(P＜0.01)，16天、24天有所減輕，但均未恢復(fù)到正常水平。(2)MCC后腦干5—羥色胺能神經(jīng)元免疫組化研究結(jié)果：TPH免疫反應(yīng)陽性表達在中縫背核、中央上核以傷后1天丌始升高，2天達到高峰，并與對照組相比有顯著性差異(p＜0.05)，24天組基本恢復(fù)：與對照組相比，中縫大核以1天組陽性反應(yīng)最強(p＜0.01)，中縫蒼白核以4天組
[Abstract]:Objective: to establish a rat model of multidle cerebral concussionMCCs and to observe the histological changes of neurons in the cortex, dorsal hippocampus, dentate gyrus and brainstem reticular structure, and the changes of serotonin neurons and nerve fibers in the cerebral cortex, dorsal hippocampus, dentate gyrus and brainstem reticular structure of MCC rats. To investigate the changes of neurons and serotonergic nerves in the brain area associated with cognitive behavioral disorders in MCC rats in order to understand the neurobiological basis of cognitive behavioral disorders in rats with triple concussion. Methods: 48 rat MCC models were established with a metal pendulum device. The rats were randomly divided into two groups: 1 day, 2 days, 4 days, 8 days, 16 days, and 24 days, 6 triple concussion groups, the rats were randomly divided into two groups: 1 day, 2 days, 4 days, 8 days, 16 days, and 24 days, 6 triple cerebral concussion groups. Normal control group (n = 8). The neurons were displayed by toluene violet staining and the pyknotic denatured neurons were counted by light microscope. The expression of serotonin neurotransmitter (5-HT) and the rate-limiting enzyme tryptophan hydroxylase (TPH) during the synthesis of 5-HT in rat brain stem were detected by immunohistochemical method. The changes of serotonin nerve fibers in cerebral cortex, septum, medial nucleus accumbens, thalamus, amygdala and hippocampus were detected by 5-HT antibody immunohistochemical method. Results the results of histochemical study on the posterior cerebral neurons of MCC were as follows: compared with the control group, the prefrontal cortex was found in the prefrontal cortex 1 day after MCC compared with the control group. The pyknotic degeneration neurons of parietal cortex and piriform cortex began to increase at the peak of 2 days group (P < 0.05). The pyknotic denaturation neurons of temporal lobe cortex reached the peak on the 2nd day after injury and decreased on the 4th day, and then recovered on the 8th day and then returned to normal on the 24th day after the injury. The number of hippocampal CA1~CA4 and upper, hypodentate gyrus pyknotic denaturation neurons began to increase 1 day after injury, the number of hippocampal CA1~CA4 and upper dentate degeneration neurons reached its peak on 2 days, and the number of inferior dentate retrograde neurons reached its peak on 16 days. There was no significant difference between 24 days and the control group. The level of pontine nucleus in brain stem reticular structure was significantly higher than that in control group (P < 0.05) except for 16 days and 24 days. The pathological changes were alleviated on the 2nd day, 4th day and 8th day after injury (P < 0.01) or 16 days after injury. In the group of 24 days after injury, the patients returned to normal basically. The most serious lesions in olivary nucleus were observed on the 2nd day, 4th day and 8th day after injury, P < 0.01 and 16 days and 24 days after injury, but they did not return to normal level. The results of immunohistochemical study of serotonin neurons in brain stem showed that the positive expression of TPH immunoreactive reaction in dorsal raphe nucleus was found in the dorsal raphe nucleus. The central superior nucleus increased to the peak at the beginning of 1 day after injury, and there was significant difference between the control group and the control group on the 24th day. Compared with the control group, the nucleus raphe Magnus showed the strongest positive reaction on the 1st day group (p < 0.01), and the pallor nucleus raphe pallidus group on the 4th day group.
【學(xué)位授予單位】：昆明醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R361

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