本文選題：酒精 + 胃黏膜　； 參考：《南京醫(yī)科大學(xué)》2006年碩士論文

【摘要】：以往的研究證實(shí)，慢性飲酒可使胃黏膜細(xì)胞更新加快，引起胃黏膜適應(yīng)性細(xì)胞保護(hù)作用，但其機(jī)制尚不清楚。本研究在以往建立的慢性飲酒動(dòng)物模型的基礎(chǔ)上，，探討長(zhǎng)期攝入低濃度酒精和胃黏膜細(xì)胞增殖之間的相關(guān)性以及與此相關(guān)的分子事件，尤其關(guān)注活性氧(reactive oxygen species，ROS)及細(xì)胞外信號(hào)調(diào)節(jié)激酶(extracellular signal-regulated kinases，ERKs)、大分子MAPK(big mitogen-activated protein kinases-1，BMK1)和c-Jun氨基末端激酶(c-Jun N-terminal kinase，JNK)信號(hào)通路是否參與了慢性飲酒引起的大鼠胃黏膜細(xì)胞增殖過程。將成年雄性SD大鼠隨機(jī)分為四組，第一組(對(duì)照組)：飲用清水；第二組(酒精組)：飲用6％(v／v)酒精水溶液；第三組(槲皮素組)：飲用清水，槲皮素(100mg／kg)每12h灌胃一次；第四組(酒精+槲皮素組)：飲用6％酒精水溶液，同時(shí)每隔12h給予槲皮素(100mg／kg)灌胃一次，均處理7天。在處理7天末，取大鼠胃黏膜，用流式細(xì)胞術(shù)、增殖細(xì)胞核抗原(PCNA)的免疫組化染色以及PCNA和細(xì)胞周期蛋白D1(CyclinD1)的免疫印跡分析觀察胃黏膜的細(xì)胞增殖情
[Abstract]:Previous studies have confirmed that chronic drinking can accelerate the regeneration of gastric mucosal cells and induce adaptive cell protection of gastric mucosa, but its mechanism is not clear. Based on the previous animal models of chronic drinking, the relationship between chronic alcohol intake and gastric mucosal cell proliferation and the related molecular events were investigated. In particular, Ros) and extracellular signal-regulated kineses (MAPK(big mitogen-activated protein kinases-1hBMK1) and c-Jun amino terminal kinase c-Jun N-terminal kinase (c-Jun) signaling pathway were involved in the proliferation of gastric mucosal cells induced by chronic drinking in rats. Adult male SD rats were randomly divided into four groups: the first group (control group: drinking clear water), the second group (alcohol group: drinking 6 v / v) alcohol aqueous solution, the third group (quercetin group: drinking water, quercetin 100 mg / kg) every 12 hours; The fourth group (alcohol quercetin group: drinking 6% alcohol aqueous solution, at the same time once every 12 hours, 100 mg / kg quercetin) was treated for 7 days. At the end of 7 days, gastric mucosa was taken from rats. The proliferation of gastric mucosa was observed by flow cytometry, immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and immunoblotting analysis of PCNA and cyclin D1CyclinD1).
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 繆錕,孫亮,LauraHTang,IrvinMModlin;大鼠胃的主細(xì)胞分離純化培養(yǎng)及功能測(cè)定[J];南京醫(yī)科大學(xué)學(xué)報(bào);2000年05期

2 沈紅芬,戈應(yīng)濱,朱學(xué)江,張小虎,王自正,袁孝如;慢性酒精刺激對(duì)大鼠下丘腦及伏核內(nèi)β-內(nèi)啡肽含量的影響[J];南京醫(yī)科大學(xué)學(xué)報(bào);2001年04期

3 戈應(yīng)濱,杜軍,田蘇平,李衛(wèi)星,顧洛;細(xì)胞更新對(duì)慢性飲酒大鼠胃黏膜適應(yīng)性細(xì)胞保護(hù)的影響[J];中國應(yīng)用生理學(xué)雜志;2005年01期

相關(guān)會(huì)議論文 前1條

1 戈應(yīng)濱;杜軍;田蘇平;李衛(wèi)星;顧洛;;適量活性氧介導(dǎo)慢性飲酒大鼠胃粘膜適應(yīng)性細(xì)胞保護(hù)[A];中國生理學(xué)會(huì)第21屆全國代表大會(huì)暨學(xué)術(shù)會(huì)議論文摘要匯編[C];2002年

本文編號(hào)：1908705