發(fā)布時間：2018-05-18 10:32

  本文選題：衰老 + 羰基應(yīng)激　； 參考：《湖南師范大學》2006年博士論文

【摘要】：隨著衰老研究的深入,研究者們對衰老的認識經(jīng)歷了由主動衰老→被動衰老,由程序性死亡→隨機損傷積累的過程;也正是因為認識論的發(fā)展,目前隨機性損傷累積的杰出代表——自由基/氧化應(yīng)激衰老理論已被認為是“第一衰老理論”。而隨著研究的深入,需氧參與的自由基氧化和不需氧參與的非酶糖基化,這兩大最重要的能量代謝相關(guān)生化副反應(yīng),都被證實與許多疾病的病理機制以及衰老密切相關(guān),而細察其兩者的作用過程,發(fā)現(xiàn)它們均產(chǎn)生具有很強生物反應(yīng)活性的毒性羰基類物質(zhì);考慮到毒性羰基類物質(zhì)本身的特點及其與生物大分子的作用方式,我們進而思考:羰基應(yīng)激是否是自由基氧化和非酶糖基化造成的衰老相關(guān)改變的直接方式呢?值此,產(chǎn)生了本學位論文——衰老過程中的羰基應(yīng)激。 根據(jù)論題,本論文的主要從兩個方面開展探索:即羰基應(yīng)激研究和去羰基應(yīng)激研究。 首先,從生化水平證實了老年色素形成過程的羰基應(yīng)激機制。通過引入前表面三維熒光技術(shù),從根本上解決了老年色素檢測的技術(shù)問題,利用該方法得到了老年色素的全波段熒光光譜信息,從而以清晰的數(shù)據(jù)徹底澄清了困擾老年色素研究長達幾十年之久的顏色之爭(藍色和黃褐色);根據(jù)前表面三維熒光數(shù)據(jù),我們進一步從簡單和復雜的體系綜合證實了:隨著老年色素的“成熟”過程,老年色素的峰出現(xiàn)了紅移;并且隨著羰基應(yīng)激的加劇,老年色素的形成也加劇,隨著羰基應(yīng)激的抑制,老年色素的形成也得到了抑制。這些充分的實驗事實表明:羰基應(yīng)激(特別是羰-氨交聯(lián))是老年色素形成的核心生化機制之一。 其次,從細胞水平發(fā)現(xiàn)了丙二醛(malondialdehyde,MDA)直
[Abstract]:With the deepening of aging research, researchers' understanding of aging has gone through the process of active senescence and passive senescence, and the accumulation of random damage caused by programmed death, which is also due to the development of epistemology. At present, the theory of free radical / oxidative stress senescence has been regarded as the "first aging theory", which is an outstanding representative of random damage accumulation. With the development of research, aerobic free radical oxidation and non-aerobic non-enzymatic glycosylation, the two most important biochemical side effects related to energy metabolism, have been proved to be closely related to the pathological mechanism of many diseases and aging. After careful examination of the processes of their action, it was found that both of them produced toxic carbonyl substances with strong biological activity, considering the characteristics of the toxic carbonyl substances themselves and the way in which they acted with biological macromolecules, We went on to wonder whether carbonyl stress was a direct way of changing senescence related to free radical oxidation and non-enzymatic glycosylation. Therefore, the carbonyl stress in the process of senescence is produced in this dissertation. According to the topic, this thesis mainly explores two aspects: carbonyl stress and decarbonyl stress. Firstly, the mechanism of carbonyl stress in the formation of aged pigments was confirmed at the biochemical level. By introducing the three dimensional fluorescence technique of the front surface, the technical problem of the detection of the aged pigment is solved fundamentally, and the full-band fluorescence spectrum information of the aged pigment is obtained by using this method. Thus, with clear data, the color dispute (blue and yellowish brown) that has plagued the study of geriatric pigments for decades (blue and yellowish brown) has been completely clarified; according to the three dimensional fluorescence data on the front surface, We further demonstrated from simple and complex systems that the peak of senile pigments shifted red with the "maturation" of pigments, and that the formation of geriatric pigments increased with the increase of carbonyl stress. With the inhibition of carbonyl stress, the formation of senile pigments was also inhibited. These sufficient experimental facts indicate that carbonyl stress (especially carbonyl-ammonia crosslinking) is one of the core biochemical mechanisms for the formation of aged pigments. Secondly, malondialdehyde (malondialdehyde) was found at cell level.
【學位授予單位】：湖南師范大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R33

【引證文獻】

相關(guān)碩士學位論文 前1條

1 石旺;紅細胞壓積水平與NAFLD風險率的相關(guān)性研究[D];湖南師范大學;2012年

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本文編號：1905497