本文選題：腸神經(jīng)系統(tǒng) + 神經(jīng)生長因子�。� 參考：《安徽醫(yī)科大學(xué)》2006年碩士論文

【摘要】：目的 研究人胚胎結(jié)腸神經(jīng)系統(tǒng)的發(fā)育過程中神經(jīng)生長因子(Nerve Growth Factor，NGF)與其兩個受體TrkA(tyrosine kinase A)、P75NTR(Nerve Growth Factor low affinity receptor P75)在結(jié)腸神經(jīng)系統(tǒng)中的分布及與結(jié)腸神經(jīng)系統(tǒng)發(fā)育的關(guān)系。方法 采用一抗為PGP9.5(protein gene product 9.5)、NGF、TrkA、P75NTR的免疫組化技術(shù)，顯示結(jié)腸神經(jīng)系統(tǒng)不同發(fā)育階段腸壁中的神經(jīng)元。結(jié)果 人胚胎發(fā)育有明顯的階段性，在不同的階段神經(jīng)生長因子(NGF)、高親和力受體TrkA、低親和力受體P75NTR在神經(jīng)細(xì)胞中的表達(dá)水平不同。在胚胎發(fā)育早期(胎齡4個月以前)，腸壁發(fā)育差，以后出現(xiàn)菲薄的平滑肌層和低平的腸粘膜，偶見NGF免疫反應(yīng)性神經(jīng)膠質(zhì)細(xì)胞；至發(fā)育中期(胎齡4-5個月)，腸壁分化出4層結(jié)構(gòu)，出現(xiàn)相當(dāng)發(fā)達(dá)絨毛，NGF、TrkA、P75NTR、PGP9.5免疫反應(yīng)性細(xì)胞明顯增多，彌散分布于整個肌層間并逐漸遷移到粘膜下層和粘膜層；至發(fā)育晚期(6-9個月)，腸壁各層均增厚，，肌間神經(jīng)叢成簇分布，神經(jīng)纖維構(gòu)成的網(wǎng)絡(luò)出現(xiàn)更為細(xì)小的3級突起，粘膜下神經(jīng)叢分化形成淺叢和深從；NGF在胞漿、突起，TrkA在胞漿、胞膜、胞核上，PGP9.5在胞漿上著色進(jìn)一步加深，免疫反應(yīng)進(jìn)一步增強(qiáng)；P75NTR免疫反應(yīng)陽性細(xì)胞數(shù)增多但著色深淺并無明顯變化，染色強(qiáng)度變化不大。結(jié)論 結(jié)腸神經(jīng)系統(tǒng)的發(fā)育有明顯的階段性。在發(fā)育早期未見NGF、TrkA、P75NTR、PGP9.5免疫反應(yīng)性神經(jīng)細(xì)胞；在發(fā)育中期NGF、TrkA、P75NTR、PGP9.5神經(jīng)成分在腸壁各層中出現(xiàn)并發(fā)育增生；晚期腸壁各層神經(jīng)成分分化成熟，NGF、TrkA、PGP9.5染色強(qiáng)度進(jìn)一步增強(qiáng)，但P75NTR染色強(qiáng)度變化不大；在神經(jīng)系統(tǒng)發(fā)育不同階段，NGF、TrkA與P75NTR比例在發(fā)生變化，在不同的發(fā)育階段P75NTR和NGF、TrkA相互作用發(fā)揮不同的生理學(xué)效應(yīng)，而在不同的發(fā)育階段，原始病因引起NGF、TrkA、P75NTR之間比例異常，將導(dǎo)致腸神經(jīng)發(fā)育異常。
[Abstract]:Objective to study the distribution of nerve growth factor (NGF) and its two receptors, TrkA(tyrosine kinase Agna P75NTRNerve Growth Factor low affinity receptor P75, in the colonic nervous system during the development of the colonic nervous system of human embryo and its relationship with the development of the colonic nervous system. Methods Immunohistochemical technique was used to display neurons in the intestinal wall of the colon nervous system at different stages of development. Results there were obvious stages in the development of human embryos. The expression levels of NGF, TrkA and P75NTR in nerve cells were different at different stages. NGF immunoreactive glial cells were occasionally found in the early stage of embryonic development (4 months before gestational age, with poor development of intestinal wall, thin smooth muscle layer and low level intestinal mucosa). At the middle stage of development (4-5 months of gestational age), the intestinal wall differentiated into four layers, and the immunoreactive cells of NGF TrkAn P75NTRN PGP9.5 increased obviously, distributed throughout the myometrium and gradually migrated to the submucous and mucous layers. In the late stage of development, every layer of the intestinal wall was thickened, the intermuscular nerve plexus was clustered, the network of nerve fibers formed a smaller three-grade process, and the submucosal plexus differentiated into superficial plexus in the cytoplasm, and the neurite TrkA in the cytoplasm, and the submucosal plexus differentiated into a superficial plexus in the cytoplasm and TrkA in the cytoplasm. The staining of PGP 9.5 on the membrane and nucleus was further deepened, and the immunoreaction was further enhanced. The number of P75NTR immunoreactive positive cells increased, but the staining depth did not change obviously, and the staining intensity did not change much. Conclusion the development of the colon nervous system has obvious stages. In the early stage of development, there were no immunoreactive nerve cells of NGF TrkAn P75NTRN PGP9.5; in the middle stage of development, the neuronal components of NGF TrkAgna P75NTRN PGP9.5 appeared and proliferated in all layers of the intestinal wall, and the staining intensity of NGFTK TrkAg PGP9.5 was further enhanced in the late stage of the differentiation and maturation of the nerve components of the various layers of the intestinal wall. However, the intensity of P75NTR staining did not change significantly, and the ratio of P75NTR TrkA to P75NTR changed in different stages of nervous system development. The interaction between P75NTR and NGF TrkA played different physiological effects in different developmental stages, but in different developmental stages. The original etiology causes abnormal ratio between NGFV TrkAgna P75 NTR and leads to abnormal development of intestinal nerve.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R321

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