本文選題：腦血管痙攣 + 兔�。� 參考：《蘇州大學(xué)》2006年碩士論文

【摘要】： 本課題分兩部分,第一部分主要研究兔血管內(nèi)穿刺腦血管痙攣(Cerebral Vasospasm, CVS)模型的病理學(xué)變化;第二部分探討血管內(nèi)皮細胞凋亡在CVS痙攣血管中的表達。 第一部份兔血管內(nèi)穿刺CVS模型的腦血管病理改變 目的:研究兔血管內(nèi)穿刺模型的腦血管痙攣時間窗和腦血管形態(tài)學(xué)變化。 材料與方法:健康清潔級新西蘭大白兔72只采用血管內(nèi)穿刺法即經(jīng)股動脈穿刺將導(dǎo)管插到兔頸內(nèi)動脈,以導(dǎo)絲刺破頸內(nèi)動脈,產(chǎn)生蛛網(wǎng)膜下腔出血(Subarachnoid Hemorrhage, SAH),制作CVS模型。存活兔子隨機分成12小時、1天、2天、3天及7天組,共5大組。每組又根據(jù)導(dǎo)絲是否刺破頸內(nèi)動脈分為SAH組(5只)及對照組(2只)。每只兔子術(shù)前、術(shù)后常規(guī)行頭顱CT檢查,SAH評估參考Fisher分級,分0-Ⅲ級。每只兔子處死后常規(guī)行HE染色觀察后交通動脈及基底動脈管腔內(nèi)直徑及管壁厚度改變。選取12只兔子(對照組2只,每SAH組各2只)的右頸內(nèi)動脈行透射電鏡(Transmission Electron Microscope, TEM)觀察血管壁超微結(jié)構(gòu)改變。 結(jié)果:對照組10只及SAH組25只兔子獲取腦組織標本。SAH組中,SAHⅠ級8只,Ⅱ級14只,Ⅲ級3只。同對照組相比,后交通動脈及基底動脈管腔內(nèi)直徑在SAH后12小時分別縮小了43.6%和51.82%。隨后在1天～7天動脈呈持續(xù)性狹窄,管腔直徑在第7天再次明顯縮小,分別下降了29.32%和45.19%。7天組后交通動脈和基底動脈管壁厚度均明顯增厚,分別增加了56.95%和44.33%。 結(jié)論:兔血管內(nèi)穿刺CVS模型呈急性期收縮和遲發(fā)性收縮雙相改變。其痙攣時間窗與血管形態(tài)學(xué)改變同人腦血管痙攣基本相仿。兔血管內(nèi)穿刺CVS模型是研究人腦血管痙攣發(fā)病機制及治療方法的適合模型。
[Abstract]:This study was divided into two parts. The first part was to study the pathological changes of Cerebral Vasospasm (CVS) model, and the second part was to investigate the expression of vascular endothelial cell apoptosis in CVS vasospasm. The first part of the CVS model of intravascular puncture in rabbits Objective: to study the changes of cerebral vasospasm time window and cerebral vascular morphology in rabbit intravascular puncture model. Materials and methods: 72 healthy and clean grade New Zealand white rabbits were inserted into the internal carotid artery by femoral artery puncture, and the subarachnoid hemorrhage (SAHN) was produced by the guide wire to puncture the internal carotid artery. The model of CVS was made by using subarachnoid hemorrhage (SAHX). Surviving rabbits were randomly divided into 5 groups: 12 hours, 1 day, 2 days, 3 days and 7 days. Each group was divided into SAH group (n = 5) and control group (n = 2). Before and after operation, each rabbit underwent routine head CT examination to evaluate the Fisher grade, which was divided into 0-鈪，

本文編號：1900850