本文選題：雙向電泳 + 質(zhì)譜��； 參考：《南京醫(yī)科大學(xué)》2005年碩士論文

【摘要】：隨著社會發(fā)展,不孕癥越來越成為困擾夫妻雙方身心健康的世界性問題。世界衛(wèi)生組織預(yù)測,在21世紀(jì),不孕癥將成為僅次于腫瘤和心腦血管病的第三大疾病。故不孕不育癥是一項(xiàng)世界性醫(yī)學(xué)和社會學(xué)的重要問題,也是當(dāng)前生命科學(xué)研究的熱點(diǎn)。隨著輔助生育技術(shù)的日益發(fā)展,越來越多的不孕癥患者由助孕的方法獲得妊娠。在對輔助生殖技術(shù)的研究過程中,人們深刻認(rèn)識到了如何得到和保留優(yōu)質(zhì)卵子的重要性。卵母細(xì)胞發(fā)生、發(fā)育、成熟的調(diào)節(jié)機(jī)制的一直是研究的焦點(diǎn)。近年來,人們對卵母細(xì)胞和卵泡細(xì)胞發(fā)育的分子機(jī)制進(jìn)行了大量的研究,但大多采用單通道模式,即對單個(gè)蛋白、基因的功能研究。蛋白質(zhì)是基因功能的實(shí)施者,蛋白質(zhì)有自身特有的活動規(guī)律,如動態(tài)修飾、加工、轉(zhuǎn)運(yùn)定位、結(jié)構(gòu)形成、代謝等,均無法從基因組水平上的研究獲知。而且卵子發(fā)育過程是一個(gè)涉及眾多信號分子的調(diào)控網(wǎng)絡(luò),包括許多已知及未知的重要蛋白參與,采用單通道的模式無法進(jìn)行高效的研究。從蛋白質(zhì)水平對卵子發(fā)育機(jī)制進(jìn)行高通量的研究是本課題的目標(biāo)。 20世紀(jì)90年代中期,國際上萌發(fā)了蛋白質(zhì)組學(xué)這一新的學(xué)術(shù)領(lǐng)
[Abstract]:With the development of society, infertility has become a world-wide problem that puzzles the physical and mental health of husband and wife. The World Health Organization predicts that infertility will become the third largest disease in the 21st century after cancer and cardiovascular and cerebrovascular diseases. So infertility is an important problem in medicine and sociology all over the world, and it is also a hot topic in life science research. With the development of assisted fertility technology, more and more infertility patients get pregnancy by the aid of pregnancy. In the course of research on assisted reproductive technology, people have realized the importance of how to obtain and retain high quality eggs. The regulation of oocyte formation, development and maturation has been the focus of research. In recent years, a great deal of research has been done on the molecular mechanism of oocyte and follicle cell development, but most of them adopt single channel mode, that is, the function of single protein and gene. Proteins are the implementers of gene function. Proteins have their own specific activity rules, such as dynamic modification, processing, transport location, structure formation, metabolism and so on. Furthermore, egg development is a regulatory network involving many signaling molecules, including many known and unknown important proteins, which can not be efficiently studied using a single channel model. High-throughput research on egg development mechanism from protein level is the goal of this paper. In the mid-1990s, proteomics, a new academic field, came into being in the world.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R321

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 喻小燕;曹晶;謝錦云;;蛋白質(zhì)組學(xué)在發(fā)育生物學(xué)研究中的應(yīng)用[J];金陵科技學(xué)院學(xué)報(bào);2006年04期

相關(guān)碩士學(xué)位論文 前1條

1 胡惠忠;豬卵母細(xì)胞雙向電泳分析方法的建立[D];廣西大學(xué);2007年

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本文編號：1899907