本文選題：中性粒細(xì)胞 + 凋亡�。� 參考：《天津醫(yī)科大學(xué)》2005年碩士論文

【摘要】：[目的] 目前膿毒癥和膿毒癥性休克仍然是危重病人的主要死亡原因之一,特別是在嬰幼兒由于其自身發(fā)育特點(diǎn),膿毒癥和膿毒癥性休克的死亡率更是居高不下。傳統(tǒng)的治療方法并不能明顯改善其預(yù)后,降低死亡率。進(jìn)一步探討其發(fā)病機(jī)理,尋求有效的治療方法是十分必要的。大量研究認(rèn)為中性粒細(xì)胞在膿毒癥時(shí)發(fā)生凋亡延遲,導(dǎo)致炎癥反應(yīng)過(guò)度,造成組織損傷是膿毒癥發(fā)展惡化的原因之一。以往研究認(rèn)為糖皮質(zhì)激素具有拮抗內(nèi)毒素的作用,但最近研究發(fā)現(xiàn)體外條件下糖皮質(zhì)激素對(duì)中性粒細(xì)胞的凋亡有延遲作用。那么,究竟糖皮質(zhì)激素在膿毒癥時(shí)對(duì)內(nèi)毒素?fù)p傷后的中性粒細(xì)胞有何作用,是保護(hù)還是損傷?這是本實(shí)驗(yàn)的目的之一。生長(zhǎng)激素是一種蛋白質(zhì)激素,以往一直被用來(lái)治療垂體性侏儒,但最近許多文獻(xiàn)報(bào)道生長(zhǎng)激素在危重癥中有積極的作用,能提高膿毒癥動(dòng)物的生存率。那么生長(zhǎng)激素對(duì)內(nèi)毒素?fù)p傷后中性粒細(xì)胞的凋亡是否也存在影響,這一影響在膿毒癥中的意義如何呢?這是本實(shí)驗(yàn)的另一個(gè)目的。我們希望通過(guò)本實(shí)驗(yàn)為臨床進(jìn)一步探討膿毒癥和膿毒癥性休克的治療提供參考。 [方法] 采用不連續(xù)密度梯度離心法分離人中性粒細(xì)胞于CO_2培養(yǎng)箱(37℃,5%CO_2)中培養(yǎng)過(guò)夜。實(shí)驗(yàn)共分8組(n=3),分別為正常對(duì)照組(N),內(nèi)毒素組(LPS),大劑量地塞米松組(DEX1),大劑量地塞米松治療組(LPS+DEX1),小劑量地塞米松組(DEX2),小劑量地塞米松治療組(LPS+DEX2),生長(zhǎng)激素組(GH),生長(zhǎng)激素治療組(LPS+GH),其中LPS的終濃度為10ug/ml,DEX1的終濃度為10~(-6)M,DEN2的終濃度為10~(-8)M,GH的終濃度為100ug/ml。每組均以3小時(shí),6小時(shí),12小時(shí),24小時(shí)作為檢測(cè)時(shí)點(diǎn)。根據(jù)實(shí)驗(yàn)設(shè)計(jì)于96
[Abstract]:[objective] sepsis and septic shock are still one of the main causes of death in critically ill patients, especially in infants due to their own developmental characteristics, sepsis and septic shock mortality is even higher. Traditional treatment can not significantly improve the prognosis and reduce mortality. It is necessary to further explore its pathogenesis and seek effective treatment. A large number of studies suggest that the delayed apoptosis of neutrophils in sepsis leads to excessive inflammatory reaction and tissue damage is one of the reasons for the development and deterioration of sepsis. Previous studies have shown that glucocorticoids have an antagonistic effect on endotoxin, but recent studies have found that glucocorticoids have delayed the apoptosis of neutrophils in vitro. So, what is the effect of glucocorticoids on neutrophils after endotoxin injury in sepsis, protection or injury? This is one of the purposes of this experiment. Growth hormone (GH) is a protein hormone, which has been used to treat pituitary dwarfism in the past. However, it has been reported in many recent literatures that it plays an active role in critical illness and can improve the survival rate of septic animals. So does growth hormone have an effect on neutrophil apoptosis after endotoxin injury, and what is the significance of this effect in sepsis? This is another purpose of this experiment. We hope to provide a reference for the further study of the treatment of sepsis and septic shock. [methods] Human neutrophils were isolated by discontinuous density gradient centrifugation and cultured overnight in CO_2 incubator at 37 鈩，

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