發(fā)布時(shí)間：2018-05-15 08:56

  本文選題：腫瘤休眠 + nm23-H1　； 參考：《四川大學(xué)》2006年碩士論文

【摘要】：目的 建立人非小細(xì)胞肺癌實(shí)驗(yàn)性裸鼠肺部的腫瘤細(xì)胞休眠模型，，并驗(yàn)證nm23-H1基因是否具有促進(jìn)腫瘤細(xì)胞休眠的作用。為深入研究腫瘤休眠的機(jī)制及治療提供研究模型。 方法 L9981-nm23-EGFP是nm23-H1基因以真核載體pEGFP質(zhì)粒轉(zhuǎn)染L9981所構(gòu)建的人非小細(xì)胞肺癌細(xì)胞株。L9981-EGFP是轉(zhuǎn)染真核空載體pEGFP質(zhì)粒的不含nm23-H1基因的人高轉(zhuǎn)移性非小細(xì)胞肺癌細(xì)胞株。我們以L9981-nm23-EGFP肺癌細(xì)胞作為實(shí)驗(yàn)組，空載高轉(zhuǎn)移性肺癌細(xì)胞株L9981-EGFP作為對(duì)照組，分別將兩組腫瘤細(xì)胞通過尾靜脈接種裸鼠，形成實(shí)驗(yàn)性非小細(xì)胞肺癌的肺部轉(zhuǎn)移模型。8周后斷頸法處死全部裸鼠，用免疫組織化學(xué)染色(SP)法檢測2組裸鼠肺部肺癌細(xì)胞的Ki-67增殖指數(shù)，TUNEL法檢測癌細(xì)胞凋亡指數(shù)，SP法檢測腫瘤組織VEGF的表達(dá)，并用流式細(xì)胞術(shù)檢測了裸鼠肺組織的調(diào)亡指數(shù)和G0／G1期細(xì)胞占細(xì)胞總數(shù)的百分比。 結(jié)果 L9981-nm23-EGFP組免疫組化增殖指數(shù)、凋亡指數(shù)和VEGF的表達(dá)均低于高轉(zhuǎn)移性L9981-EGFP組。流式細(xì)胞術(shù)檢測裸鼠肺組織的凋亡指數(shù)亦支持免疫組化的結(jié)果。實(shí)驗(yàn)組G0／G1期細(xì)胞占細(xì)胞總數(shù)的比率
[Abstract]:Objective to establish a tumor cell dormancy model of human non-small cell lung cancer (NSCLC) in nude mice and to verify whether nm23-H1 gene can promote tumor cell dormancy. To provide a model for the further study of the mechanism and treatment of tumor dormancy. Methods L9981-nm23-EGFP is a human non-small cell lung cancer cell line. L9981-EGFP constructed by transfection of nm23-H1 gene with eukaryotic vector pEGFP plasmid. L9981-EGFP is a highly metastatic human non-small cell lung cancer cell line without nm23-H1 gene transfected into eukaryotic vector pEGFP plasmid. L9981-nm23-EGFP lung cancer cells were used as experimental group and non-loaded high metastatic lung cancer cell line L9981-EGFP as control group. Two groups of tumor cells were inoculated into nude mice via tail vein respectively. The lung metastasis model of experimental non-small cell lung cancer (NSCLC) was established. After 8 weeks, all nude mice were killed by cervical amputation. The Ki-67 proliferation index (Ki-67) of lung cancer cells in two groups of nude mice was detected by immunohistochemical staining and the expression of VEGF was detected by Tunel method. The apoptotic index of lung tissue and the percentage of cells in G0/G1 phase in the lung tissue of nude mice were measured by flow cytometry. Results the immunohistochemical proliferative index, apoptosis index and VEGF expression in L9981-nm23-EGFP group were lower than those in high metastatic L9981-EGFP group. The apoptotic index of lung tissue in nude mice by flow cytometry also supported the immunohistochemical results. Ratio of G0/G1 phase cells to total cells in experimental group
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R734.2;R-332

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 黃旭暉;健脾化瘀方促進(jìn)小鼠肝癌休眠的機(jī)制研究[D];廣州中醫(yī)藥大學(xué);2013年

本文編號(hào)：1891803