發(fā)布時(shí)間：2018-05-13 06:10

  本文選題：腸球菌 + esp基因　； 參考：《南華大學(xué)》2005年碩士論文

【摘要】：目的:檢測(cè)腸球菌臨床分離株對(duì)6種抗菌藥物的敏感性,分析糞腸球菌臨床株的esp基因擴(kuò)增序列,以揭示腸球菌臨床株的耐藥現(xiàn)狀及esp基因與糞腸球菌耐藥的關(guān)系,為臨床合理選用藥物治療腸球菌感染提供實(shí)驗(yàn)及理論依據(jù)。 方法:從臨床實(shí)驗(yàn)室收集腸球菌感染標(biāo)本,進(jìn)行分純、培養(yǎng)、鑒定;用瓊脂平板稀釋法檢測(cè)去甲萬(wàn)古霉素、氨芐西林、環(huán)丙沙星、阿奇霉素和紅霉素對(duì)腸球菌的最低抑菌濃度(MIC)及鑒定慶大霉素高濃度(MIC≥500μg/mL)耐藥株,篩選出對(duì)6種抗菌藥物呈不同程度耐藥或多重耐藥的腸球菌。然后用PCR法擴(kuò)增糞腸球菌臨床株的esp基因;隨機(jī)挑選一陽(yáng)性菌株,將其PCR產(chǎn)物克隆到pUC18載體,轉(zhuǎn)化大腸桿菌JM109,藍(lán)白斑篩選挑取陽(yáng)性重組子進(jìn)行限制性內(nèi)切酶酶切、PCR及測(cè)序鑒定,并對(duì)測(cè)序結(jié)果用Blast軟件進(jìn)行同源性分析,探討糞腸球菌耐藥與esp基因的關(guān)系。 結(jié)果:從臨床分離的腸球菌共78株,其中糞腸球菌61株,占78.2%;屎腸球菌15株,占19.2%;鳥腸球菌、堅(jiān)韌腸球菌各1株,共占2.6%。 去甲萬(wàn)古霉素對(duì)腸球菌的MIC_(50)、MIC_(90)分別為0.μg/mL和4μg/mL;氨芐西林和環(huán)丙沙星的MIC_(50)、MIC_(90)分別為1μg/mL和4μg/mL、16μg/mL和64μg/mL:阿奇霉素和紅霉素的MIC_(50)、MIC_(90)較高,分別為16μg/mL和32μg/mL、64μg/mL和128μg/mL。未檢測(cè)出去對(duì)甲萬(wàn)古霉素耐藥的腸球菌,腸球菌對(duì)氨芐西林、環(huán)丙沙星、高濃度慶大霉素、阿奇霉素和紅霉素的耐藥率分別為16.7%、53.8%、56.4%、74.4%、85.9%。 糞腸球菌和屎腸球菌對(duì)氨芐西林的耐藥率分別為9.8%和46.7%(P0.01);對(duì)環(huán)丙沙星的耐藥率分別為50.8%和73.3%(P0.05),這兩種主要腸球菌對(duì)氨芐
[Abstract]:Objective: to detect the susceptibility of clinical isolates of Enterococcus faecalis to 6 kinds of antimicrobial agents, and analyze the amplified sequence of esp gene of Enterococcus faecalis clinical strains, so as to reveal the current situation of drug resistance of Enterococcus faecalis and the relationship between esp gene and drug resistance of Enterococcus faecalis. To provide experimental and theoretical basis for clinical rational selection of drugs for enterococcal infection. Methods: enterococcal infection specimens were collected from clinical laboratory, isolated, cultured and identified, and norvancomycin, ampicillin and ciprofloxacin were detected by Agar plate dilution method. The minimal inhibitory concentration (MIC) of azithromycin and erythromycin against Enterococcus spp. Then the esp gene of Enterococcus faecalis clinical strain was amplified by PCR, and a positive strain was selected randomly, and its PCR product was cloned into pUC18 vector, and transformed into E. coli JM109. The positive recombinant was screened and identified by restriction endonuclease digestion and sequencing. The results of sequencing were analyzed by Blast software to investigate the relationship between drug resistance of Enterococcus faecalis and esp gene. Results: a total of 78 strains of Enterococcus faecalis, 15 strains of Enterococcus faecium, 1 strain of Enterococcus tenacity and 1 strain of Enterococcus tenacity, accounted for 78.2%, 19.2% and 2.6%, respectively, were isolated from clinic. The mice of ciprofloxacin and ampicillin are 1 渭 g/mL and 4 渭 g / mL, respectively, which are 1 渭 g/mL and 4 渭 g / mL, 16 渭 g/mL and 64 渭 g / mL, respectively, and 16 渭 g / mL and 64 渭 g / mL of azithromycin and erythromycin, respectively, which are 16 渭 g/mL and 32 渭 g / mL, 64 渭 g/mL and 128 渭 g / mL respectively. The resistance rates of Enterococcus to ampicillin, ciprofloxacin, high concentration of gentamicin, azithromycin and erythromycin were 16.73.8and 56.4%, respectively. The resistance rates of Enterococcus to ampicillin, ciprofloxacin and erythromycin were 56.4% and 85.9%, respectively. The resistance rates of Enterococcus faecalis and Enterococcus faecium to ampicillin were 9.8% and 46.7%, respectively, and those to ciprofloxacin were 50.8% and 73.3%, respectively.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R378

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

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本文編號(hào)：1881993