本文選題：乳桿菌 + 女性陰道�。� 參考：《遼寧師范大學(xué)》2005年碩士論文

【摘要】：本論文目的在于從健康女性陰道中分離并鑒定產(chǎn)生細(xì)菌素、產(chǎn)酸能力強(qiáng)的優(yōu)勢(shì)乳桿菌,并對(duì)其益生特性作初步研究。分離得到得19 株乳桿菌通過(guò)菌落、菌株形態(tài)觀察以及多項(xiàng)生理生化等常規(guī)鑒定,并且通過(guò)16S　rRNA 基因序列測(cè)序鑒定,確定這19 株乳桿菌分屬于乳桿菌屬(Lactobacillus)內(nèi)專性同型發(fā)酵類型中三個(gè)不同的種。其中1-1、1-2、1-3、1-7、51-1、51-4、52-1-1、52-1-2、52-3 共9 株為格氏乳桿菌( Lactobacillus　gasseri ), 23-2-1 為唾液乳桿菌　　(Lactobacillus.salivarius),23-2-2、23-4、23-5、23-8、32-3、46-1、46-2、46-3、50-3 共9 株為卷曲乳桿菌(Lactobacillus.crispatus)�！� 這19 株乳桿菌發(fā)酵過(guò)程中都產(chǎn)生細(xì)菌素,除了32-3,51-1,51-4 這3 株菌外,都產(chǎn)生過(guò)氧化氫。其36h 發(fā)酵液的pH 值都在4 左右。對(duì)革蘭氏陽(yáng)性指示菌有強(qiáng)烈的抑制作用,對(duì)革蘭氏陰性菌以及白色念珠菌均有一定的抑制作用。在桿菌與乳酸球菌以及桿菌之間的拮抗作用實(shí)驗(yàn)中,桿菌對(duì)同一生境下的乳酸球菌幾乎沒有抑制作用,桿菌之間,除菌株46-2 對(duì)其他的桿菌有抑制作用外,其他的桿菌之間沒有抑制作用。　 因此這些從人體中分離出來(lái)的乳桿菌優(yōu)勢(shì)菌群,具有多種維持特殊生境健康的因素,這些作用協(xié)同放大,在微環(huán)境中會(huì)具有強(qiáng)烈的抑制病原菌的功能。本論文所研究的乳桿菌均來(lái)源于人體,如應(yīng)用于人體作為微生態(tài)制劑則會(huì)具有強(qiáng)大的優(yōu)勢(shì),符合當(dāng)前女性特殊生境保健的“人性化”潮流�！�
[Abstract]:The purpose of this paper is to isolate and identify the dominant Lactobacillus that produce bacteriocin and produce acid from healthy female vagina, and to study its probiotic characteristics. 19 strains of Lactobacillus were identified by colony, morphological observation, physiological and biochemical analysis, and 16s rRNA gene sequencing. The 19 strains of Lactobacillus belong to three different species of homozygous fermentations of Lactobacillus. Among them, 9 strains were Lactobacillus gravis (Lactobacillus gasseri, 23-2-1 was Lactobacillus saliva Lactobacillus, 23-2-2 was Lactobacillus saliva 23-2-2, 23-523-523-532-346-36-46-46-46-3Con 50-3 was Lactobacillus crispatus.Then 9 strains were Lactobacillus crispatusus. All the 19 Lactobacillus strains produced bacteriocin and hydrogen peroxide, except for the three strains of 32-3, 51-1 and 51-4, all of which produced hydrogen peroxide. The pH value of the fermentation broth for 36 h was about 4. It had strong inhibitory effect on Gram-positive indicator bacteria, Gram-negative bacteria and Candida albicans. In the antagonistic experiment between Bacillus lactis and Lactococcus and Bacillus, Bacillus had little inhibitory effect on Lactococcus in the same habitat, but between strains 46-2 had inhibitory effect on other bacilli. There was no inhibition between other bacteria. Therefore, these dominant strains of Lactobacillus isolated from the human body have a variety of factors to maintain the health of special habitats. These effects are co-amplified and have a strong function of inhibiting pathogenic bacteria in microenvironment. The Lactobacillus studied in this paper all come from the human body. If it is applied to the human body as a microecological agent, it will have a strong advantage, which is in line with the current trend of "humanization" in the health care of the female special habitat.
【學(xué)位授予單位】：遼寧師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R37

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 李少英;奶牛源性雙歧桿菌和乳桿菌的分離鑒定及耐藥性研究[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2008年

相關(guān)碩士學(xué)位論文 前2條

1 張艾青;產(chǎn)廣譜細(xì)菌素植物乳桿菌的初步研究及其在泡菜中的應(yīng)用[D];四川農(nóng)業(yè)大學(xué);2007年

2 劉婷婷;健康婦女陰道乳桿菌的分離及鑒定[D];蘭州大學(xué);2010年

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本文編號(hào)：1880737