本文選題：間充質干細胞 + Notch信號通路��； 參考：《中國實驗血液學雜志》2014年04期

【摘要】：本研究旨在探討Notch信號傳導通路在VEGF促大鼠間充質干細胞增殖中的作用。體外培養(yǎng)大鼠間充質干細胞,取對數(shù)生長期的細胞用于實驗研究。用Notch通路阻斷劑DAPT阻斷Notch信號傳導,觀察該通路在VEGF作用下大鼠間充質干細胞的增殖情況。實驗分為4組:空白對照組、VEFG組、DAPT組及VEGF+DAPT組。應用CCK-8法檢測各組細胞增殖情況,RT-PCR法檢測各組相關基因(Notch1、Notch2、Flk-1、HES-1)mRNA水平的變化。結果表明,細胞培養(yǎng)3 d,各時間點(24 h,48 h,72 h)DAPT組及VEGF+DAPT組細胞存活率均較低,細胞形態(tài)變圓脫落,數(shù)目明顯減少;VEGF組存活率最高,差異具有統(tǒng)計學意義(P0.01);與對照組相比,VEGF組的Notch1、Notch2和Flk-1的表達水平均上調,而DAPT組及VEGF+DAPT組Notch1和Notch2的表達水平均下調,差異均具有統(tǒng)計學意義(P0.05);VEGF組Hes-1基因水平下調,而DAPT組及VEGF+DAPT組Hes-1基因水平上調,差異具有統(tǒng)計學意義(P0.05);DAPT組及VEGF+DAPT組的Flk-1基因表達水平稍有下調,但差異無統(tǒng)計學意義(P0.05)。結論:Notch信號通路在VEGF作用下對大鼠間充質干細胞的增殖具有明顯促進作用,而DAPT可抑制這種增殖效應。
[Abstract]:The aim of this study was to investigate the role of Notch signaling pathway in the proliferation of rat mesenchymal stem cells stimulated by VEGF. Rat mesenchymal stem cells were cultured in vitro. Notch signal transduction was blocked by DAPT, a Notch pathway blocker, and the proliferation of rat mesenchymal stem cells induced by VEGF was observed. The experiment was divided into 4 groups: blank control group (VEFG) and VEGF DAPT group. CCK-8 assay was used to detect cell proliferation in each group. RT-PCR was used to detect the mRNA level of Notch1ntch2Flk-1pHES-1mRNA. The results showed that the cell survival rate was lower, the cell morphology became round and shedded, and the number of cells decreased significantly at 24 h or 48 h ~ 72 h)DAPT, and the survival rate was the highest in the VEGF DAPT group at 3 days after cell culture. Compared with the control group, the expression of Notch1ntch2 and Flk-1 were up-regulated, while the expression of Notch1 and Notch2 were down-regulated in DAPT group and VEGF DAPT group. However, the level of Hes-1 gene was up-regulated in DAPT group and VEGF DAPT group, and the difference was statistically significant. The expression of Flk-1 gene in DAPT group and VEGF DAPT group was slightly down-regulated, but the difference was not statistically significant (P 0.05). Conclusion the VEGF can promote the proliferation of rat mesenchymal stem cells by VEGF, and DAPT can inhibit the proliferation of rat mesenchymal stem cells.
【作者單位】： 廣東醫(yī)學院附屬醫(yī)院兒童醫(yī)學中心;
【基金】：湛江市財政資金科技專項競爭性分配項目(2012C0302)
【分類號】：R329.2

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本文編號：1878416