本文選題：EB病毒 + Rta蛋白　； 參考：《四川大學(xué)》2005年碩士論文

【摘要】：[背景和目的] EB病毒(Epstein-Barr virus,EBV)是γ皰疹病毒的一種,與鼻咽癌(nasopharyngeal carcinoma,NPC)、霍奇金氏病(Hodgkin's disease,HD)、等多種惡性腫瘤密切相關(guān),被國際癌癥研究中心列為病毒性致瘤因子。EB病毒的生活周期可分為潛伏期和裂解期兩個階段,其中裂解期又可細(xì)分為立即早期、早期、病毒DNA復(fù)制期和晚期。Rta蛋白是EB病毒裂解立即早期基因BRLF1的編碼產(chǎn)物,是EB病毒由潛伏期轉(zhuǎn)向裂解期的關(guān)鍵性調(diào)控因子之一。它能引起一系列裂解早期基因的相繼表達(dá),最終引發(fā)EB病毒裂解感染。此外,Rta蛋白和細(xì)胞因子之間的相互作用在調(diào)節(jié)宿主細(xì)胞分化增殖方面也發(fā)揮了重要的作用。本研究旨在制各針對EB病毒Rta蛋白的高效價和特異性的多克隆抗體,并初步應(yīng)用于EB病毒相關(guān)腫瘤組織中Rta蛋白表達(dá)情況的研究。 [方法] (1)將已構(gòu)建的兩個原核表達(dá)質(zhì)粒pGEX-R185I和pGEX—R150I
[Abstract]:[background and purpose] Epstein-Barr virus EBV (Epstein-Barr virus) is a kind of 緯 herpesvirus, which is closely related to nasopharyngeal carcinoma (NPC) and Hodgkinia disease (HD). The life cycle of Epstein-Barr virus (EBV) as a viral tumorigenic factor by the International Center for Cancer Research can be divided into two stages: latent period and lytic period, in which the lytic period can be subdivided into immediate early stage and early stage. Virus DNA replication and late. Rta protein is the encoding product of EBV's immediate early gene BRLF1, and it is one of the key regulatory factors of EBV from latent period to lytic stage. It can cause successive expression of a series of early lytic genes, and eventually lead to Epstein-Barr virus lytic infection. In addition, the interaction between Rta protein and cytokines also plays an important role in regulating the differentiation and proliferation of host cells. The purpose of this study was to prepare specific polyclonal antibodies against EB virus Rta protein and to study the expression of Rta protein in Epstein-Barr virus-associated tumor tissues. [methods] Two prokaryotic expression plasmids pGEX-R185I and pGEX-R150I were constructed.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392

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本文編號：1851057