本文選題：Ii抗原 + IGnTc��； 參考：《揚(yáng)州大學(xué)》2006年碩士論文

【摘要】： Ii血型系統(tǒng)是第27個(gè)紅細(xì)胞血型系統(tǒng)。它們沒有自己的編碼基因,i、I抗原分別由線性和分支型多聚N-乙酰半乳糖胺決定,因此其抗原決定簇為糖分子。新生兒臍血紅細(xì)胞攜帶i抗原,隨著生長(zhǎng)發(fā)育,β-1,6-N-乙酰半乳糖胺轉(zhuǎn)移酶活性的出現(xiàn)將2個(gè)N-乙�；樘前�(N-AC)分別加到i抗原的線性多聚N-乙酰半乳糖胺結(jié)構(gòu)的3和6位,從而使直鏈i抗原轉(zhuǎn)變?yōu)榉种虸抗原。 本研究選用臍血紅細(xì)胞做免疫原,用i(+)和i(-)紅細(xì)胞進(jìn)行篩選,得到三株抗i單克隆抗體。通過純化i單克隆抗體(IgG)作為免疫原,獲得了抗i的羊抗鼠二抗�？筰單克隆抗體及其二抗的研制為檢測(cè)稀有血型ii,提供了必備的檢測(cè)試劑。 通過RT-PCR的方法成功地從胎盤組織中克隆了β-1,6-N-乙酰半乳糖胺轉(zhuǎn)移酶基因(IgnTc),并構(gòu)建了重組真核表達(dá)載體pcDNA3.1-IgnTc。重組質(zhì)粒轉(zhuǎn)染CHO細(xì)胞,使IgnTc在CHO細(xì)胞中的成功表達(dá)。IgnTc活性的出現(xiàn)將CHO細(xì)胞表面的i抗原轉(zhuǎn)變?yōu)镮抗原,獲得了I陽性的CHO細(xì)胞株,為篩選抗I單克隆抗體及臨床鑒定血清中相關(guān)I抗體的提供了重要的工具。 本研究的實(shí)施將進(jìn)一步確保輸血安全,給輸血患者帶來福音,還將為在中國(guó)開展稀有血型的檢測(cè)、普查工作,建立稀有血型庫奠定基礎(chǔ)。
[Abstract]:I blood group system is the 27 th red blood group system. They do not have their own coding gene, I. the antigens are determined by linear and branched polyN-acetylgalactosamine respectively, so their antigen determinants are sugar molecules. Umbilical cord blood red blood cells carried I antigen. With the development of growth and development, the activity of 尾 -1n 6-N-acetylgalactosamine transferase was added to the third and sixth position of linear polyN-acetylgalactosamine structure of I antigen, respectively. Thus the straight chain I antigen was transformed into branched I antigen. In this study, cord blood red blood cells were used as immunogen. The goat anti-mouse anti-I second antibody was obtained by purification of I monoclonal antibody (IgG) as immunogen. The development of anti-I monoclonal antibody and its second antibody provides a necessary reagent for the detection of rare blood group ii. The 尾 -1G 6-N-acetylgalactosamine transferase gene was cloned from placenta tissue by RT-PCR, and the recombinant eukaryotic expression vector pcDNA3.1-IgnTc. was constructed. The recombinant plasmid was transfected into CHO cells. The successful expression of IgnTc in CHO cells resulted in the transformation of I antigen from the surface of CHO cells to I antigen, and the I-positive CHO cell line was obtained. It provides an important tool for the screening of anti-I monoclonal antibodies and clinical identification of anti-I antibodies in serum. The implementation of this study will further ensure the safety of blood transfusion, bring good news to patients with blood transfusion, and lay the foundation for the testing and general investigation of rare blood groups in China and the establishment of a database of rare blood groups.
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

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1 洪光;血型Ii單克隆抗體的研制及羊抗鼠二抗的制備[D];揚(yáng)州大學(xué);2006年

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本文編號(hào)：1834746