本文選題：脊髓半側(cè)液壓損傷 + 動物模型　； 參考：《天津醫(yī)科大學(xué)》2007年博士論文

【摘要】： 目的：1、開發(fā)小動物立體定向固定系統(tǒng)和適合制備小動物脊髓半側(cè)損傷模型的打擊管及連接裝置。2、建立小動物脊髓半側(cè)損傷標準化的操作方法。3、使用國際通用的液壓沖擊損傷儀，建立一種損傷能量精確、重復(fù)性好、損傷分級明確、并發(fā)癥和死亡率低的脊髓半側(cè)損傷模型。4、建立評價脊髓半側(cè)損傷動物模型的理想評價指標(包括行為、電生理、影像、病理)。為深入研究脊髓損傷后的病理、生理變化過程及治療效果的驗證提供穩(wěn)定的實驗平臺。 方法：1、64只SD大鼠分為損傷組(n=48)，對照組(n=16)，損傷組按照損傷能量分為輕、中、重3個亞組(每組n=16)。2、所有大鼠均采用標準手術(shù)步驟操作，行T10右側(cè)椎板開骨窗。3、大鼠固定于自行開發(fā)的小動物實驗立體定向固定系統(tǒng)，連接打擊管道，密封排水，利用液壓打擊系統(tǒng)在特定打擊能量下制作輕、中、重各級脊髓損傷模型。4、各組于建模后3、7、14天各取3只動物行實驗性脊髓損傷神經(jīng)功能評分、運動誘發(fā)電位、磁共振、病理檢查及免疫組化檢查，對模型進行評價；另7只動物行連續(xù)觀察至14天，進行動態(tài)評估。 結(jié)果：1、本研究開發(fā)的小動物立體定向固定系統(tǒng)具有操作簡單固定可靠的特點，可保證大鼠在麻醉清醒后牢固固定，并確保致傷打擊方向一致，打擊管及連接管道密閉性良好，避免了打擊過程中因漏水而發(fā)生致傷力衰減。2、脊髓損傷節(jié)段準確，根據(jù)損傷能量的不同可穩(wěn)定地分別制備出輕、中、重型脊髓半側(cè)損傷模型，各組間神經(jīng)功能評分、運動誘發(fā)電位評價指標與損傷程度顯著相關(guān)，且各組間上述指標具的差異具有統(tǒng)計學(xué)意義(P＜0.05)。磁共振、病理結(jié)果可明確顯示不同程度的傷后改變。3、隨時間延長模型動物神經(jīng)功能可部分恢復(fù)。4、本組動物損傷后的自然死亡率為8％，尿儲留等并發(fā)癥發(fā)生率為14％，相對較少。 結(jié)論：1、自行研制的小動物立體定向固定系統(tǒng)和小動物脊髓損傷專用打擊管及連接裝置穩(wěn)定性好，可使液壓損傷能量按設(shè)計方向精確地作用于脊髓的特定位置，提高了模型的準確性和可重復(fù)性。2、本研究建立的脊髓半側(cè)液壓損傷動物模型較為接近人體脊髓損傷自然過程，，并且能夠精確控制損傷能量，較好地制備出不同程度的脊髓半側(cè)損傷模型。3、采用神經(jīng)功能評分、運動誘發(fā)電位、磁共振、病理檢查方法可對損傷程度和恢復(fù)狀況進行精確評估。4、該方法保留部分二便功能，并發(fā)癥少，動物長期存活率高。
[Abstract]:Objective: to develop a stereotactic fixation system for small animals, and to establish a standardized operating method of spinal cord injury in small animals by using the universal hydraulic impingement instrument, which is suitable for the establishment of a small animal spinal cord injury model and its connecting device .2.The purpose of this paper is to establish a standardized operation method of spinal cord injury in small animals. To establish a spinal cord injury model with accurate energy, good reproducibility, clear injury grade, low complication and mortality, and to establish an ideal evaluation index (including behavior, electrophysiology, imaging) for evaluating the animal model of spinal cord hemilateral injury. Pathology. To provide a stable experimental platform for the further study of pathology, physiological process and therapeutic effect of spinal cord injury. Methods Sixty-four Sprague-Dawley rats were divided into injury group (n = 48) and control group (n = 16). The rats were fixed to the experimental stereotactic fixation system of small animals developed by ourselves, connected to the striking pipe, sealed and drained, and made light, medium and light by hydraulic hitting system under the specific strike energy, the rats were fixed on the right side of the vertebral lamina of T10, and the rats were fixed in the experimental stereotactic fixation system developed by ourselves. The model of spinal cord injury was severe. 3 animals were taken from each group on the 3rd day and 14th day after modeling to evaluate the experimental spinal cord injury nerve function score, motor evoked potential, magnetic resonance imaging, pathological examination and immunohistochemical examination. The other 7 animals were observed for 14 days for dynamic evaluation. Results: the stereotactic fixation system of small animals developed in this study has the characteristics of simple and reliable operation, which can ensure that the rats can be firmly fixed after anaesthesia, and ensure that the direction of injury is the same, and the airtight of the pipe and the connecting pipe is good. The injury force decreased by 0.2 due to leakage during the attack, and the segment of spinal cord injury was accurate. According to the different energy of injury, the models of light, medium and severe spinal cord injury could be made stably, and the neurological function scores of each group could be obtained. The evaluation index of motor evoked potential was significantly correlated with the degree of injury, and the difference of the above indexes among the groups was statistically significant (P < 0.05). Magnetic resonance imaging (MRI), pathological results showed the changes of different degrees after injury. The neurological function of model animals recovered partly with time. The natural mortality rate was 8, and the incidence of complications such as urinary retention was 14, relatively less. Conclusion the self-developed stereotactic fixation system for small animals and the special hitting tube and connecting device for spinal cord injury in small animals have good stability and can make the hydraulic injury energy act precisely on the specific position of spinal cord according to the design direction. The accuracy and repeatability of the model were improved. The animal model of spinal cord hemiplegic injury established in this study is close to the natural process of spinal cord injury and can accurately control the injury energy. The model of spinal cord hemilateral injury with different degrees was made. The nerve function score, motor evoked potential, magnetic resonance and pathological examination can be used to accurately evaluate the degree of injury and recovery. There are few complications and high long-term survival rate.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2007
【分類號】：R651.2;R-332

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相關(guān)期刊論文 前2條

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