本文選題：CD新生小鼠 + 視網(wǎng)膜母細胞瘤��； 參考：《武警醫(yī)學(xué)》2014年12期

【摘要】：目的建立視網(wǎng)膜母細胞瘤(retinoblastoma,RB)模型,為研究RB新療法提供實驗動物模型。方法選取出生第1天的CD1小鼠40只,隨機分為A、B、C、D 4組,每組10只。設(shè)每組小鼠的右眼為實驗眼,左眼為對照眼,將RB-Y79細胞以4μl/只注射至右眼玻璃體腔內(nèi),A、B、C、D組注射的RB-Y79濃度分別是2.0×107/ml、3.0×107/ml、3.5×107/ml、4.0×107/ml。4組小鼠左眼均注射等量重懸細胞的溶劑;觀察小鼠生長狀態(tài)及移植瘤生長情況,并與對照眼比較,選擇出最佳建模細胞濃度;取出的組織常規(guī)石蠟切片HE染色,觀察移植瘤組織細胞形態(tài)學(xué)特征。結(jié)果注射RB-Y79細胞后,實驗小鼠右眼可形成腫瘤,而對照眼左眼無腫瘤形成;注射的RB-Y79細胞濃度不同,成瘤率和存活率不同,第10天A、B、C、D組的成瘤率分別為20%、80%、100%和100%,存活率分別為100%、80%、80%和50%。C組3.5×107/ml為最佳建模濃度;移植瘤組織病理檢查:右眼眼球結(jié)構(gòu)被破壞。瘤細胞大小不等,形態(tài)為圓形、橢圓形、多邊形或不規(guī)則形;細胞質(zhì)少;胞核大,圓形,卵圓形或不規(guī)則形,染色深,有1~2個以上核樣結(jié)構(gòu)。結(jié)論新生小鼠玻璃體腔內(nèi)注射RB-Y79細胞可成功建立RB小鼠模型。
[Abstract]:Objective to establish retinoblastoma RBB model for the study of new RB therapy. Methods Forty CD1 mice on the first day of birth were randomly divided into 4 groups, 10 in each group. The right eye of each group was used as experimental eye and the left eye as control. RB-Y79 cells were injected into the vitreous cavity of right eye by 4 渭 l / L only. The concentration of RB-Y79 in group D was 2.0 脳 107 / ml, 3.0 脳 107 / ml, 3.5 脳 10 ~ (7) / ml ~ 4.0 脳 107/ml.4, respectively, and the same amount of solvent was injected into the left eye of mice. The growth state of mice and the growth of transplanted tumor were observed, and the optimal concentration of model cells was selected compared with the control eyes, and the morphological characteristics of transplanted tumor were observed by HE staining of paraffin sections. Results after injection of RB-Y79 cells, tumor formation was observed in the right eye of the experimental mice, but no tumor was found in the left eye of the control eye, and the tumor forming rate and survival rate were different with different concentrations of RB-Y79 cells injected. On the 10th day, the tumorigenesis rate of group D was 20% and 100%, the survival rate was 100% and 50%, respectively, and the best modeling concentration was 3.5 脳 107/ml in group C and group C, respectively. Histopathological examination of transplanted tumor showed that the structure of right eye was destroyed. The size of tumor cells varied from round, elliptic, polygonal or irregular; the cytoplasm was small; the nucleus was large, round, oval or irregular, and the staining depth was deep, and there were more than 1 ~ 2 nucleus-like structures. Conclusion RB mouse model can be successfully established by intravitreal injection of RB-Y79 cells in newborn mice.
【作者單位】： 徐州醫(yī)學(xué)院;武警總醫(yī)院兒科;
【分類號】：R739.7;R-332

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本文編號：1824920