本文選題：毛首線蟲屬 + 分類學(xué)��； 參考：《重慶醫(yī)科大學(xué)》2005年碩士論文

【摘要】：本研究從重慶市動物園阿拉伯狒狒和松鼠猴體內(nèi)收集了兩種毛首線蟲成蟲。為了初步鑒定蟲種并探討這兩種毛首線蟲與已知六種毛首線蟲的親緣距離,我們對這兩種毛首線蟲進(jìn)行了形態(tài)學(xué)鑒別和分子生物學(xué)方法的分類學(xué)研究。 本研究利用測微尺對蟲體體長、前部長、后部長、輸精管長、射精管長、泄殖腔長、交合刺長、交合刺鞘長,交合刺鞘刺高度等(測量單位為 mm)形態(tài)學(xué)體征進(jìn)行了測量并顯微鏡拍照。根據(jù)所獲得的測量數(shù)據(jù)和顯微鏡觀察雄蟲生殖器官形態(tài)學(xué)特點,與已知蟲種進(jìn)行比對,做出形態(tài)學(xué)初步鑒別。隨后,抽提兩種毛首線蟲的 DNA,通過 PCR 擴(kuò)增出核糖體 DNA(rDNA)中的 ITS2 片斷,進(jìn)行測序,并使用 DNAMAN等軟件與已公布的六種毛首線蟲的 ITS2 數(shù)據(jù)比較親緣距離等數(shù)據(jù),進(jìn)行分類學(xué)研究。最后,將 PCR 產(chǎn)物純化,選用 MspⅠ和 HaeⅢ兩種限制性內(nèi)切酶對純化產(chǎn)物進(jìn)行 RFLP 分析,并獲得酶切圖譜。 結(jié)果顯示,這兩種不同來源的毛首線蟲在形態(tài)學(xué)上有明顯區(qū)別,其中阿拉伯狒狒來源的毛首線蟲測量數(shù)據(jù)在 T. cynocephalus(皮翼毛首線蟲)參考范圍內(nèi),雄蟲生殖器官外形特征與 T. cynocephalus(皮翼毛首線蟲)相近,初步鑒定為 T. cynocephalus(皮翼毛首線蟲)。松鼠猴來源毛首線蟲的測量數(shù)據(jù)和對雄蟲生殖器官的觀察結(jié)果表明這種毛首線蟲與 T. rhinopiptheroxell-a sp.nov(川金絲猴新種毛首線蟲)相似,由于獲得松鼠猴來源毛首線蟲成蟲少于形態(tài)學(xué)鑒別所需成蟲數(shù)的最小值(≥10 條),所以無法確定,疑為 T. rhinopiptheroxell-a sp.nov(川金絲
[Abstract]:Two species of adult nematodes were collected from Arabian baboon and squirrel monkey in Chongqing Zoo. In order to identify the two species and to study the relationship between the two species and six known species of nematode, we studied the morphological identification and molecular biological methods of the two species of nematode. In this study, the length of the body, the former minister, the posterior head, the vas deferens, the ejaculatory duct, the cloaca, the intersecting spines and the sheaths of the intersecting spines were measured by micrometer. The morphologic signs, such as height of spiny spines (measured in mm), were measured and photographed under microscope. According to the measured data and microscopic observation, the morphological characteristics of male reproductive organs were compared with those of known species, and the morphological characteristics were preliminarily identified. Then, the DNA of two species of nematodes was extracted, and the ITS2 fragments of ribosomal DNA were amplified by PCR, sequenced, and compared with the published ITS2 data of six species of nematode by using DNAMAN and other software. Carry out taxonomic research. Finally, the PCR products were purified and two restriction endonucleases, Msp 鈪，

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