本文選題：毛首線蟲(chóng)屬 + 分類(lèi)學(xué)��； 參考：《重慶醫(yī)科大學(xué)》2005年碩士論文

【摘要】：本研究從重慶市動(dòng)物園阿拉伯狒狒和松鼠猴體內(nèi)收集了兩種毛首線蟲(chóng)成蟲(chóng)。為了初步鑒定蟲(chóng)種并探討這兩種毛首線蟲(chóng)與已知六種毛首線蟲(chóng)的親緣距離,我們對(duì)這兩種毛首線蟲(chóng)進(jìn)行了形態(tài)學(xué)鑒別和分子生物學(xué)方法的分類(lèi)學(xué)研究。 本研究利用測(cè)微尺對(duì)蟲(chóng)體體長(zhǎng)、前部長(zhǎng)、后部長(zhǎng)、輸精管長(zhǎng)、射精管長(zhǎng)、泄殖腔長(zhǎng)、交合刺長(zhǎng)、交合刺鞘長(zhǎng),交合刺鞘刺高度等(測(cè)量單位為 mm)形態(tài)學(xué)體征進(jìn)行了測(cè)量并顯微鏡拍照。根據(jù)所獲得的測(cè)量數(shù)據(jù)和顯微鏡觀察雄蟲(chóng)生殖器官形態(tài)學(xué)特點(diǎn),與已知蟲(chóng)種進(jìn)行比對(duì),做出形態(tài)學(xué)初步鑒別。隨后,抽提兩種毛首線蟲(chóng)的 DNA,通過(guò) PCR 擴(kuò)增出核糖體 DNA(rDNA)中的 ITS2 片斷,進(jìn)行測(cè)序,并使用 DNAMAN等軟件與已公布的六種毛首線蟲(chóng)的 ITS2 數(shù)據(jù)比較親緣距離等數(shù)據(jù),進(jìn)行分類(lèi)學(xué)研究。最后,將 PCR 產(chǎn)物純化,選用 MspⅠ和 HaeⅢ兩種限制性?xún)?nèi)切酶對(duì)純化產(chǎn)物進(jìn)行 RFLP 分析,并獲得酶切圖譜。 結(jié)果顯示,這兩種不同來(lái)源的毛首線蟲(chóng)在形態(tài)學(xué)上有明顯區(qū)別,其中阿拉伯狒狒來(lái)源的毛首線蟲(chóng)測(cè)量數(shù)據(jù)在 T. cynocephalus(皮翼毛首線蟲(chóng))參考范圍內(nèi),雄蟲(chóng)生殖器官外形特征與 T. cynocephalus(皮翼毛首線蟲(chóng))相近,初步鑒定為 T. cynocephalus(皮翼毛首線蟲(chóng))。松鼠猴來(lái)源毛首線蟲(chóng)的測(cè)量數(shù)據(jù)和對(duì)雄蟲(chóng)生殖器官的觀察結(jié)果表明這種毛首線蟲(chóng)與 T. rhinopiptheroxell-a sp.nov(川金絲猴新種毛首線蟲(chóng))相似,由于獲得松鼠猴來(lái)源毛首線蟲(chóng)成蟲(chóng)少于形態(tài)學(xué)鑒別所需成蟲(chóng)數(shù)的最小值(≥10 條),所以無(wú)法確定,疑為 T. rhinopiptheroxell-a sp.nov(川金絲
[Abstract]:Two species of adult nematodes were collected from Arabian baboon and squirrel monkey in Chongqing Zoo. In order to identify the two species and to study the relationship between the two species and six known species of nematode, we studied the morphological identification and molecular biological methods of the two species of nematode. In this study, the length of the body, the former minister, the posterior head, the vas deferens, the ejaculatory duct, the cloaca, the intersecting spines and the sheaths of the intersecting spines were measured by micrometer. The morphologic signs, such as height of spiny spines (measured in mm), were measured and photographed under microscope. According to the measured data and microscopic observation, the morphological characteristics of male reproductive organs were compared with those of known species, and the morphological characteristics were preliminarily identified. Then, the DNA of two species of nematodes was extracted, and the ITS2 fragments of ribosomal DNA were amplified by PCR, sequenced, and compared with the published ITS2 data of six species of nematode by using DNAMAN and other software. Carry out taxonomic research. Finally, the PCR products were purified and two restriction endonucleases, Msp 鈪，

本文編號(hào)：1824154