本文選題：鎳 + 大鼠　； 參考：《蘭州大學》2007年碩士論文

【摘要】： 目的：植入鎳(Nickel，Ni)及鎳鈦(Ni Ti shape memory alloy，NiTi)合金于大鼠后肢股部，模擬人體合金植入，以純金鎳作為高劑量鎳離子(Ni~(2+))釋放組，合金組為低劑量釋放組，觀察植入物持續(xù)鎳離子(Ni~(2+))釋放對大鼠生殖和胚胎發(fā)育的影響，探討含鎳金屬鎳離子(Ni~(2+))釋放對雌鼠生殖能力的影響，以及含鎳金屬對植入局部的病理影響和生物學特點，揭示金屬鎳體內緩慢腐蝕釋放對大鼠生殖過程和局部組織的毒理影響，進一步了解含鎳金屬假體在生物體內緩慢離子釋放的生物安全度。 方法：大鼠隨機分為假手術組(Sham operated group)、鎳組(Nickel group)、合金組(Allay group)；分別植入金屬鎳5g/Kg體重及鎳鈦合會10g/Kg體重于股部，使鎳組和合金組植入鎳含量總量相同，配對后分籠常規(guī)飼養(yǎng)，觀察Ni~(2+)在體內持續(xù)釋放對大鼠受孕和胚胎發(fā)育的影響，計數(shù)受孕率、活胎率、死胎率、胎吸收率，計算胎兒體重，重要臟器系數(shù)、新生鼠體重增長及對植入部位局部組織的影響，對主要臟器的病理學影響等。 結果：鎳組大鼠的著床數(shù)、活胎率較假手術組和合金組低(P＜0.05)，死胎率高于假手術組和合金組(P＜0.05)，胎鼠的體重和肝、肺、腎臟器系數(shù)鎳組低于假手術組和合金組(P＜0.05)，生后第1d、5d新生鼠體重鎳組低于假手術組和合金組(P＜0.05)，10、15、20d新生鼠各組無顯著性差異。局部組織在早期40d時形成炎性肉芽腫及其壞死，中期80d時為局部囊性組織增生和血性滲出液，組織類型為橫紋肌水腫，肌纖維紊亂，，粗大，內容物為破碎紅細胞及少量淋巴細胞，晚期160d時局部包塊增大，囊壁增厚分隔，病理組織類型為胚胎型橫紋肌肉瘤，可見大量均勻的深染癌細胞，內容物為大量異常的間皮細胞和散落的癌細胞。胎鼠臟器肉眼解剖均未發(fā)現(xiàn)明顯結構異常；鎳組死亡胎鼠面顱和腦顱均發(fā)育較差，存活鎳組新生鼠未見異常。光鏡可見一例鎳組胎鼠視網(wǎng)膜畸形，余組未見異常；股骨頭軟骨發(fā)育鎳組較合金組和假手術組差，表現(xiàn)為軟骨增殖區(qū)同源細胞減少。鎳組新生鼠生存能力下降，死亡數(shù)增加，體重增長減慢。
[Abstract]:Objective: to implanted nickel (Nickel, Ni) and nickel titanium (Ni Ti shape memory alloy, NiTi) alloy in the femoral part of the hind limbs of rats to simulate human alloy implantation and YISHION gold nickel as a high dose nickel ion (Ni~ (2+)) release group. The alloy group was a low dose release group. The effects of the release of the implanted nickel ions (Ni~ (2+)) on the reproductive and embryonic development of rats were observed. The effects of nickel metal nickel ions (Ni~ (2+)) on the reproductive capacity of female rats, and the pathological and biological characteristics of nickel containing metal to the local implantation, reveal the toxicological effects of the slow corrosion release of nickel metal on the reproductive process and local tissues of the rats, and further understand the birth of the slow ion release of the nickel bearing metal prosthesis in the organism. Safety of things.
Methods: rats were randomly divided into sham operation group (Sham operated group), nickel group (Nickel group) and alloy group (Allay group). The nickel and titanium alloy group were implanted in the femoral part, respectively. The nickel content and the alloy group were implanted in the same amount of nickel in the nickel group and the alloy group, and the normal feeding of Ni~ (2+) in the body was observed. The effects of pregnancy and embryo development, counting the pregnancy rate, the rate of live fetus, the rate of stillbirth, fetal absorption, calculating the fetal weight, the important organ coefficient, the weight growth of the newborn rats and the effect on the local tissue of the implanted parts, and the pathological effects on the main organs.
Results: the number of implantation in the nickel group was lower than that of the sham operation group and the alloy group (P < 0.05). The stillbirth rate was higher than that of the sham operation group and the alloy group (P < 0.05). The weight of the fetal rat, the liver, lung, and kidney factor nickel group were lower than the sham operation group and the alloy group (P < 0.05), the postnatal 1D, and the 5D newborn rats were lower than the sham operation group and the alloy group (P < 0.05), 1 There was no significant difference in 0,15,20d neonatal rats. The local tissue formed inflammatory granuloma and necrosis at early 40d. The medium 80D was localized cystic tissue hyperplasia and hemorrhagic exudate, the type of tissue was rhabdomyedema, muscle fiber disorder, and coarse, the contents were broken red cells and a small number of lymphocytes, and the local mass increased in the late 160D. The pathological tissue type was embryonal rhabdomyosarcoma, and a large number of homogeneous deep dyed cancer cells were found. The contents were a large number of abnormal mesothelial cells and scattered cancer cells. There were no obvious structural abnormalities in the naked eyes of fetal rats. The retinal malformation in a nickel group was seen in a nickel group. The nickel group of the femoral head cartilage was less than the alloy group and the sham group, showing a decrease in the homologous cells in the cartilage proliferation area. The survival ability of the newborn rats was decreased, the number of death was increased, and the weight growth slowed down.

【學位授予單位】：蘭州大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R363

【引證文獻】

相關碩士學位論文 前2條

1 王獻文;劑量和孕期對鎳在大鼠體內分布及胎盤轉運的影響[D];蘭州大學;2009年

2 趙娜娟;鎳污染區(qū)大氣PM_(2.5)中幾種元素對不良妊娠結局的影響及胎盤屏障作用研究[D];蘭州大學;2012年

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