本文選題：長(zhǎng)角血蜱 + 雌蜱　； 參考：《河北師范大學(xué)》2005年博士論文

【摘要】：蜱類是專性吸血的外寄生節(jié)肢動(dòng)物,傳播多種人、畜疾病,給人類健康和畜牧業(yè)發(fā)展帶來極大危害。唾液腺是蜱體內(nèi)的一個(gè)重要器官,在其生命活動(dòng)中起重要作用。它不僅能維持體內(nèi)離子、水分平衡,使蜱成功吸血,而且又是各類疾病傳播的窗口。因此,對(duì)其研究具有重要的理論和實(shí)踐意義。 本文以廣泛分布于我國(guó)的長(zhǎng)角血蜱Haemaphysalis longicornis Neumann雌蜱為研究對(duì)象,利用生物化學(xué)、激素研究和蛋白質(zhì)組學(xué)等現(xiàn)代生物學(xué)前沿技術(shù)對(duì)不同發(fā)育期唾液腺蛋白質(zhì)的動(dòng)態(tài)變化、保幼激素和蛻皮激素對(duì)唾液腺是否有影響等方面進(jìn)行了較為系統(tǒng)的研究,并對(duì)雌蜱唾液腺蛋白質(zhì)組進(jìn)行了初步分析,填補(bǔ)了該領(lǐng)域中的不足,為深入開展蜱類唾液腺研究提供了依據(jù)。主要研究結(jié)果如下: 1.唾液腺蛋白含量在不同發(fā)育期發(fā)生變化。饑餓期蛋白含量低(9.51 μg/tick);吸血2d蛋白含量急劇增加(34.90 μg/tick);交配期達(dá)到峰值(84.40 μg/tick),約為饑餓期的8.8倍;飽血后蛋白含量明顯下降,飽血后2d和飽血后4d的含量分別為39.47 μg/tick和20.54 μg/tick。 饑餓雌蜱唾液腺有17條蛋白帶;吸血2d增至23條;交配后達(dá)到26條,直至飽血后4d保持不變,吸血和交配均能刺激新蛋白的合成。各期唾液腺蛋白中有主帶10條,分子量分別為192 kDa、158 kDa、136 kDa、133 kDa、129 kDa、97 kDa、84 kDa、74 kDa、45 kDa和32 kDa,主帶在不同發(fā)育期發(fā)生增加、缺失和量的變化。 2.保幼激素(JH Ⅲ)對(duì)雌蜱唾液腺蛋白含量和成分有作用,并隨發(fā)育期和劑量的不同而產(chǎn)生不同效應(yīng)。吸血2d用JH Ⅲ處理,10μg劑量使交配期唾液腺蛋白含量明顯增加,10μg、50μg和100μg劑量抑制112 kDa蛋白基因的表達(dá);飽血當(dāng)天處理,10μg、50μg和100μg劑量均能顯著提高飽血后2d唾液腺蛋白含量,100μg劑量使28 kDa蛋白基因表達(dá)和67 kDa蛋白基因上調(diào)表達(dá),高劑量200μg和400μg使236 kDa和112 kDa的蛋白缺失;飽血當(dāng)天處理,1μg和10μg劑量均能顯著提高飽血后4d雌蜱唾液腺蛋白含量,400μg劑量則顯著降低飽血后4d雌蜱唾液腺蛋白含量,100μg、200μg和400μg
[Abstract]:Tick is a special blood sucking parasite arthropod, spreading a variety of human and animal diseases, to human health and the development of animal husbandry bring great harm. Salivary gland is an important organ in ticks and plays an important role in their life activities. It can not only maintain the ion and water balance in the body and make ticks absorb blood successfully, but also serve as a window for the spread of various diseases. Therefore, its research has important theoretical and practical significance. The dynamic changes of salivary gland proteins in different developmental stages of Haemaphysalis longicornis (Haemaphysalis longicornis Neumann) were studied by using modern biological frontier techniques, such as biochemistry, hormone research and proteomics, which were widely distributed in China. The effects of juvenile hormone and ecdysone on salivary gland were studied systematically, and the proteome of female salivary gland was analyzed, which filled the deficiency in this field. It provides a basis for the further study of tick salivary glands. The main findings are as follows: 1. The content of salivary gland protein changes at different stages of development. The protein content in hunger stage was 9.51 渭 g / tickg, the protein content in 2-day blood sucking was increased sharply (34.90 渭 g / kg), the peak value of protein in mating stage was 84.40 渭 g / kg / kg, which was 8.8 times higher than that in starvation period, the content of protein decreased obviously after feeding, the content of protein was 39.47 渭 g/tick and 20.54 渭 g / kg / kg after feeding 2 days and 4 days after feeding, respectively. There were 17 protein bands in the salivary glands of female starved ticks, 23 in 2 days after feeding, 26 after mating, and no change until 4 days after feeding. Both bloodsucking and mating could stimulate the synthesis of new proteins. There were 10 main bands in the salivary gland proteins in each stage, the molecular weight of which was 192kDa 158kDa 158kDa 136kDa 133kDa-129kDa-129kDa-97 kDa-84kDa-74 kDa-45 kDa and 32kDa. the main bands increased, deletion and quantity changed at different stages of development. 2. The effect of JH 鈪，

本文編號(hào)：1817621