本文選題：中國(guó)小型豬 + 心肌梗死　； 參考：《中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院》2005年博士論文

【摘要】：目的:探討運(yùn)用球囊導(dǎo)管封堵中國(guó)小型豬冠狀動(dòng)脈來(lái)建立心肌梗死模型的方法研究。方法:選用中國(guó)小型豬20頭,麻醉后經(jīng)股動(dòng)脈或頸總動(dòng)脈置入球囊導(dǎo)管至冠狀動(dòng)脈左前降支(LAD)的第二對(duì)角支(D2)遠(yuǎn)端部位,先行封堵預(yù)處理3～4次,然后再封堵LAD的血流120min。觀察心電圖、心肌酶、心臟二維超聲檢查及冠狀動(dòng)脈造影。并允許動(dòng)物被飼養(yǎng)6～8周。結(jié)果:存活的18頭豬均完成冠狀動(dòng)脈左前降支遠(yuǎn)端的封堵,心電圖顯示急性心肌梗死典型圖形變化,血漿心肌酶明顯升高并呈動(dòng)態(tài)演變;術(shù)后1h超聲檢查出現(xiàn)室間隔前部及前壁心尖局部運(yùn)動(dòng)異常,提示成功地建立了急性心肌梗死模型。AMI術(shù)后3至5周復(fù)查顯示左心室前壁、心尖部心室壁和室間隔異常運(yùn)動(dòng);AMI術(shù)后存活的17頭豬均為陳舊性心肌梗死模型。術(shù)后保持封堵的冠狀動(dòng)脈通暢:與開(kāi)胸法相比更接近人體冠脈溶栓或急診冠脈血管成形術(shù)后心肌梗死的發(fā)展?fàn)顟B(tài),具有創(chuàng)傷小、動(dòng)物生存時(shí)間長(zhǎng)并易于術(shù)后飼養(yǎng)等優(yōu)點(diǎn)。但有1頭豬死于手術(shù)后期。另2頭豬分別在堵閉60～100min因心室纖顫搶救無(wú)效死亡。結(jié)論:運(yùn)用球囊導(dǎo)管封堵冠狀動(dòng)脈LDA可成功建立小型豬心肌梗死模型。 目的:許多需要手術(shù)治療的老年病人都伴隨有心肺等重要器官的疾病,使其手術(shù)的風(fēng)險(xiǎn)大大增加,不良的心血管事件是圍術(shù)期發(fā)病率和死亡率升高的主要原因。我們利用陳舊性心肌梗死的小型豬,采用開(kāi)胸手術(shù)構(gòu)建圍術(shù)期的實(shí)驗(yàn)動(dòng)物模型和觀察心臟結(jié)構(gòu)及功能的損害,證明在體及手術(shù)結(jié)束時(shí)實(shí)驗(yàn)?zāi)Ｐ偷挠行院头�(wěn)定性。方
[Abstract]:Objective: to study the method of using balloon catheter to occlude coronary artery of Chinese miniature pig to establish myocardial infarction model. Methods: after anesthesia, 20 Chinese miniature pigs were placed into the distal part of the second diagonal branch of coronary artery via femoral artery or common carotid artery into the second diagonal branch of left anterior descending coronary artery (lad) after anesthesia. The LAD blood flow was then blocked for 120 minutes. Electrocardiogram, myocardial enzyme, two-dimensional echocardiography and coronary angiography were observed. Animals are allowed to be kept for 6 to 8 weeks. Results: the left anterior descending coronary artery was occluded in all of the 18 surviving pigs. The electrocardiogram showed that the typical pattern of acute myocardial infarction was changed, and the plasma myocardial enzyme increased significantly and showed dynamic evolution. At 1 hour after operation, abnormal local movement of anterior ventricular septum and anterior wall was observed, which suggested that acute myocardial infarction model was successfully established. 3 to 5 weeks after AMI, left ventricular anterior wall was demonstrated by reexamination. All the 17 surviving pigs with abnormal ventricular wall and septal movement after AMI were old myocardial infarction models. Compared with open chest method, it is closer to the development of myocardial infarction after coronary thrombolysis or emergency coronary angioplasty, and has the advantages of less trauma, longer survival time and easy to be reared after operation. But one pig died after the operation. The other two pigs died of ventricular fibrillation in occluded 60~100min. Conclusion: the myocardial infarction model of miniature pig can be successfully established by balloon catheter occlusion of coronary artery LDA. Objective: many elderly patients in need of surgical treatment are accompanied by diseases such as heart, lung and other important organs, which greatly increase the risk of surgery. Adverse cardiovascular events are the main reasons for the increase of perioperative morbidity and mortality. In order to prove the validity and stability of the experimental model in vivo and at the end of the operation, we used old myocardial infarction miniature pigs to construct perioperative experimental animal model and observe the damage of cardiac structure and function. Square
【學(xué)位授予單位】：中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類(lèi)號(hào)】：R-332;R654.2

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