本文選題：骨髓間充質(zhì)干細胞 + 細胞培養(yǎng)　； 參考：《南昌大學》2006年博士論文

【摘要】：皮膚是阻止微生物、化學物質(zhì)等侵入以維持內(nèi)環(huán)境穩(wěn)定的屏障，燒傷主要損害是皮膚壞死，大面積皮膚缺損可引起全身的改變。因而創(chuàng)面處理是燒傷救治的重要環(huán)節(jié)之一，，大面積皮膚缺損創(chuàng)面的覆蓋與修復一直是臨床醫(yī)師倍感棘手的問題。燒傷創(chuàng)面的修復是一個十分復雜的生物學過程，包括炎癥反應、細胞增殖和遷移、膠原與細胞外基質(zhì)的沉積以及再上皮化等。目前，燒傷創(chuàng)面修復方法主要是以局部治療為主。大面積深度燒傷患者由于自體皮源不足，多年來運用網(wǎng)狀植皮、郵票植皮、小皮片移植、微粒植皮等技術來擴大皮膚覆蓋面積，取得了較好的臨床效果。但這些方法皮膚擴增面積有限，難以滿足大面積皮膚缺損患者盡早封閉創(chuàng)面的需求，在加快創(chuàng)面愈合的速度和改進創(chuàng)面愈合質(zhì)量，從而在減少功能障礙的發(fā)生率和提高患者生活質(zhì)量方面，目前常規(guī)應用的這些方法顯然還存在著很多不足。 組織工程學的興起和發(fā)展，為臨床解決這一難題提供了嶄新的思路和途徑。組織工程將體外分離、培養(yǎng)的高濃度的功能相關的活細胞種植于天然的、人工合成的支架上，使之植入人體后能夠形成新的有功能的組織，來制造、保存或修復失去的組織功能。組織工程化人體組織或器官產(chǎn)業(yè)化生產(chǎn)的一個關鍵因素是植入足夠數(shù)量的、不引起免疫反應的、具有再生活力的細胞。其中種子細胞的體外培養(yǎng)擴增成為組織工程學中的重要環(huán)節(jié)。理想的種子細胞應具有容易獲得，容易體外培養(yǎng)增殖，長期傳代不改變生物學特征，抗原性小，組織修復能力強等特征。目前，有關皮膚種子細胞的研究主要集中在表皮細胞大規(guī)模培養(yǎng)和表皮干細胞研究，以及胚胎干細胞向表皮細胞定向誘導分化等幾個方面。表皮細胞
[Abstract]:Skin is a barrier to prevent the invasion of microbes and chemicals in order to maintain the stability of the internal environment. The main injury of burn is skin necrosis, and a large area of skin defect can cause systemic changes. Therefore, wound management is one of the most important links in the treatment of burn, and the covering and repairing of large area skin defect is always a thorny problem for clinicians. Burn wound repair is a very complex biological process, including inflammation, cell proliferation and migration, collagen and extracellular matrix deposition and re-epithelialization. At present, local treatment is the main method to repair burn wounds. Due to the shortage of autologous skin source in large area deep burn patients, the techniques of reticular skin grafting, stamp skin grafting, small skin graft and microparticle skin grafting have been used for many years to expand the area of skin coverage, and good clinical results have been obtained. However, these methods can not meet the needs of large area skin defect patients to close the wounds as early as possible, in order to accelerate the speed of wound healing and improve the quality of wound healing. In order to reduce the incidence of dysfunction and improve the quality of life of patients, the current routine use of these methods obviously still have a lot of shortcomings. The rise and development of tissue engineering provide a new way to solve this problem. Tissue engineering plants high concentrations of function-related living cells isolated in vitro, cultured on a natural, synthetic scaffold, so that they can be implanted into the human body to form new functional tissues that can be manufactured. Save or repair lost organizational functions. A key factor in the industrial production of tissue or organs in tissue engineering is the implantation of sufficient numbers of regenerative cells that do not cause immune response. The in vitro culture and amplification of seed cells has become an important link in tissue engineering. The ideal seed cells should be easy to obtain, easy to culture and proliferate in vitro, no change of biological characteristics in long-term passage, small antigenicity, strong tissue repair ability and so on. At present, the researches on skin seed cells mainly focus on the large-scale culture of epidermal cells, the study of epidermal stem cells, and the directional induction and differentiation of embryonic stem cells into epidermal cells. Epidermal cell
【學位授予單位】：南昌大學
【學位級別】：博士
【學位授予年份】：2006
【分類號】：R329.2;R644

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