發(fā)布時間：2018-04-25 21:32

  本文選題：大腸埃希菌 + 整合子��； 參考：《四川大學(xué)》2005年碩士論文

【摘要】：目的 監(jiān)測大腸埃希菌臨床分離株的耐藥性,了解大腸埃希菌中整合子的存在頻率、類型,以及基因盒的種類和排列,觀察細菌攜帶基因盒與耐藥表型的關(guān)系,以及整合子在不同基因型菌株中的分布,探討整合子-基因盒系統(tǒng)介導(dǎo)和傳播細菌耐藥性的分子機制。 方法 利用全自動細菌分析儀,對94株大腸埃希菌臨床分離株進行確認、產(chǎn)ESBLs判讀和20種抗生素敏感性測定,紙片擴散法對另外4種抗生素進行敏感性測定;應(yīng)用PCR方法,擴增總DNA和質(zhì)粒DNA上Ⅰ類整合子、Ⅱ類和Ⅲ類整合酶;對大小相同的Ⅰ類整合子進行酶切分析;對純化后的Ⅰ類整合子進行DNA測序,將DNA序列在GeneBank中搜索,確定Ⅰ類整合子可變區(qū)基因盒的種類和排列;利用脈沖場電泳(PFGE)對大腸埃希菌進行分子分型。 結(jié)果 細菌對15種抗生素的耐藥率超過50%,耐藥率高的抗生素包括氨芐青霉素(94%)、氧哌嗪青霉素(87%)等,45株(47.9%)細菌產(chǎn)ESBLs,90株(95.7%)細菌為多重耐藥菌(耐受3種以上
[Abstract]:Purpose To monitor the drug resistance of clinical isolates of Escherichia coli, to understand the frequency and type of integron in Escherichia coli and the type and arrangement of gene boxes, and to observe the relationship between bacterial cassette and phenotype of drug resistance. And the distribution of integron in different genotypes, and the molecular mechanism of integron-gene box system mediated and spread bacterial drug resistance. Method Using automatic bacterial analyzer, 94 clinical isolates of Escherichia coli were confirmed, ESBLs interpretation and 20 antibiotic sensitivity tests, disk diffusion method were used to determine the sensitivity of the other 4 antibiotics, and PCR method was used. The class 鈪，

本文編號：1803048