本文選題：韁核 + 晝夜節(jié)律�。� 參考：《吉林大學》2007年碩士論文

【摘要】： 位于上丘腦的韁核在中樞占有顯要位置,是邊緣前腦至腦干的重要驛站。韁核與晝夜節(jié)律起搏器視交叉上核(SCN)有往返的纖維投射,并與松果體在形態(tài)和功能上有密切的聯(lián)系,這兩個部位是晝夜節(jié)律產生和調節(jié)的重要區(qū)域。韁核本身也有視網膜纖維投射,而且韁核神經元活動具有明顯的晝夜節(jié)律性,這些都表明韁核有可能是哺乳動物晝夜節(jié)律系統(tǒng)中的重要組成部分。γ-氨基丁酸(GABA)是?SCN?中主要的神經遞質,是介導晝夜節(jié)律的重要成分。在韁核內有豐富的GABA,而且內側韁核缺乏?GABA_A受體,外側韁核具有GABA_A受體,這進一步提示外側韁核在晝夜節(jié)律調節(jié)當中的重要作用。 本實驗采用全細胞膜片鉗技術,在韁核腦片上,電壓鉗模式下,在晝夜周期不同時相(白天:10:00-18:00,夜間:20:00-4:00)記錄自發(fā)興奮性突觸后電流,并觀察γ-氨基丁酸對韁核自發(fā)興奮性突觸后電流的影響。實驗結果表明:1、外側韁核自發(fā)性興奮性突觸后電流的發(fā)放頻率具有明顯的晝夜節(jié)律性,即白天高,夜間低;而內側韁核自發(fā)性興奮性突觸后電流的發(fā)放頻率無晝夜節(jié)律性。2、在外側韁核GAB_A對自發(fā)興奮性突觸后電流的影響具有節(jié)律性,白天抑制,晚上興奮;而在內側韁核GABA對興奮性突觸后電流的影響無晝夜節(jié)律性,白天和晚上均為抑制。3、GAB_A對外側韁核興奮性突觸后電流的影響主要是由GABA_A受體介導的。是什么機制導致GABA對外側韁核白天和夜間作用不同還需要進一步研究。
[Abstract]:The habenular nucleus located in the superior thalamus occupies a prominent position in the center and is an important post station from the limbic forebrain to the brain stem. The habenular nucleus and SCN of the circadian rhythm pacemaker have a round trip fibrous projection and a close relationship with the pineal body in morphology and function. These two parts are important regions for the production and regulation of circadian rhythm. The habenular nucleus itself also has retinal fiber projections and the obvious circadian rhythm of habenular nucleus neuron activity, which suggests that habenular nucleus may be an important part of the circadian rhythm system of mammals. A major neurotransmitter that mediates circadian rhythms. There is abundant GABA in habenular nucleus, and the medial habenular nucleus lacks GABA A receptor, and the lateral habenular nucleus has GABA_A receptor, which further indicates that the lateral habenular nucleus plays an important role in the regulation of circadian rhythm. In this study, whole-cell patch clamp technique was used to record spontaneous excitatory postsynaptic currents on habenula slices and in voltage-clamp mode at different periods of day and night (10: 00-18: 00, 20: 00-4: 00 at night). The effect of 緯-aminobutyric acid on spontaneous excitatory postsynaptic current in habenula was observed. The results showed that the frequency of spontaneous excitatory postsynaptic currents in the lateral habenular nucleus had a significant circadian rhythm, i.e. high during the day and low at night. The frequency of spontaneous excitatory postsynaptic currents in the medial habenular nucleus had no circadian rhythm. The effects of GAB_A on spontaneous excitatory postsynaptic currents in the lateral habenular nucleus were rhythmic and inhibited during the day and excited at night. The effect of GABA on the excitatory postsynaptic current in the medial habenular nucleus was not circadian rhythmic. During the day and at night, the inhibitory effect of GABA A on the excitatory postsynaptic current in the lateral habenular nucleus was mainly mediated by the GABA_A receptor. What causes GABA to have different daytime and nocturnal effects on the lateral habenular nucleus needs further study.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R33

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