本文選題：階段特異性胚胎抗原 + 胚胎生殖干細(xì)胞��； 參考：《蘇州大學(xué)》2005年碩士論文

【摘要】：目的:組織塊培養(yǎng)法培養(yǎng)人胚胎生殖干細(xì)胞(EG 細(xì)胞),通過檢測細(xì)胞所表達(dá)的階段特異性胚胎抗原1 和3(stage-specific embryonic antigen-1、3 ,SSEA-1、SSEA-3),對未分化的人EG 細(xì)胞進行特異性的生物學(xué)鑒定。 方法:取5—9 周流產(chǎn)人胚胎的背側(cè)腸系膜和生殖腺嵴,進行組織塊體外培養(yǎng),在培養(yǎng)液中不添加細(xì)胞因子,以同源胚胎的成纖維細(xì)胞作為飼養(yǎng)層細(xì)胞,利用免疫細(xì)胞化學(xué)的方法,對組織塊培養(yǎng)的細(xì)胞表達(dá)的SSEA-1、SSEA-3 進行檢測;取5-9 周人胚胎生殖腺嵴做石蠟切片,采用免疫組織化學(xué)的方法,對生殖腺嵴中的原始生殖細(xì)胞(primordial germ cells,PGCs)進行SSEA-1、SSEA-3 的檢測。 結(jié)果:石蠟切片免疫組織化學(xué)方法顯示,5-9 人周胚胎生殖腺嵴中有大量成條索狀排列、SSEA-1、SSEA-3 呈陽性表達(dá)的PGCs;體外培養(yǎng)過程中,在胚胎成纖維細(xì)胞上生長的單個EG 樣細(xì)胞及其集落,均表達(dá)SSEA-1、SSEA-3。 結(jié)論:本實驗組織塊培養(yǎng)所獲細(xì)胞來源于PGCs,為未分化的人EG 細(xì)胞。
[Abstract]:Objective: to culture human embryonic reproductive stem cells (EG) cells by tissue culture, and to identify the undifferentiated human EG cells by detecting the expression of phase specific embryonic antigen 1 and 3(stage-specific embryonic antigen-1p3 SSEA-1SSEA-3. Methods: the dorsal mesenteric and gonadal crest of human embryo aborted for 5-9 weeks were cultured in vitro with tissue mass. The fibroblasts of homologous embryos were used as feeder layer cells without cytokines in the culture medium. The expression of SSEA-1and SSEA-3 was detected by immunocytochemistry, and the gonadal cristae of human embryos of 5-9 weeks were taken as paraffin sections, and the expression of SSEA-3 was detected by immunohistochemical method. The primordial germ cells of primordial germ cells were detected by SSEA-1and SSEA-3 in gonadal crest. Results: the paraffin section immunohistochemical method showed that there were a large number of PGCspositive expression of SSEA-1and SSEA-3 in the gonadal crest of 5-9 human periembryonic embryos, and a single EG-like cell and its colony were grown on the embryonic fibroblasts during the process of culture in vitro. Both expressed SSEA-1 and SSEA-3. Conclusion: the cells derived from PGCswere undifferentiated human EG cells.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R321

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本文編號：1786578