本文選題：葉酸 + 維生素B_(12)��； 參考：《云南師范大學(xué)》2005年碩士論文

【摘要】：葉酸是人體正常發(fā)育的重要微營養(yǎng)物質(zhì),其涉及dUMP到dTMP的合成, 同時(shí)通過同型半胱氨酸(HC)合成蛋氨酸(Met)、S-腺苷蛋氨酸(SAM)的生 化過程而影響DNA甲基化。因此,葉酸、維生素B_(12)(B_(12))缺乏以及Met/SAM 合成受阻,可引起dUMP積累并摻入DNA,從而導(dǎo)致各種DNA結(jié)構(gòu)損傷;同 時(shí)可影響細(xì)胞尤其DNA甲基化過程,從而影響基因表達(dá)、染色體分離。 本研究以胞質(zhì)阻斷微核分析(CBMN)研究了葉酸對(duì)維護(hù)乳腺癌患者及其 對(duì)照個(gè)體淋巴細(xì)胞基因組穩(wěn)定性的作用及差異,并探討了葉酸代謝途徑中的重 要分子Met、B_(12)缺乏對(duì)BRCAI突變的乳腺癌患者成淋巴細(xì)胞株及正常人成淋 巴細(xì)胞株遺傳毒性效應(yīng)。 研究首先從24例乳腺癌患者、30例正常人外周血中分離淋巴細(xì)胞,在含 30nM、120nM、240nM 葉酸的RPMI-1640 培養(yǎng)基中(8%透析小牛血清、100U 雙抗、2mM谷氨酰胺、pH7.0)培養(yǎng),第8天以細(xì)胞松弛素B(CB)阻斷細(xì)胞 質(zhì)分裂,28小時(shí)后收獲細(xì)胞,以CMBN分析葉酸缺乏對(duì)人類淋巴細(xì)胞產(chǎn)生的遺 傳毒性效應(yīng):為了證實(shí)Met、B_(12)對(duì)葉酸代謝和遺傳穩(wěn)定性的影響,試驗(yàn)還以15,50 μM 的Met與75～1200 pM B_(12)組合的12種RPMI-1640 培養(yǎng)基(8%透析小牛血清、 100U 雙抗、2mM 谷氨酰胺、pH7.0),培養(yǎng)攜帶BRCAI,基因突變的乳腺癌患者 成淋巴細(xì)胞株GM13705和正常人成淋巴細(xì)胞株GM12593,第8天以細(xì)胞松弛素 B(CB)阻斷細(xì)胞質(zhì)分裂,28小時(shí)后收獲細(xì)胞,以CMBN分析Met、B_(12)對(duì)人類 成淋巴細(xì)胞株基因組穩(wěn)定性的影響。 試驗(yàn)結(jié)果表明: 1、在本試驗(yàn)濃度范圍內(nèi),葉酸濃度與乳腺癌患者及其對(duì)照個(gè)體淋巴細(xì)胞遺傳損 傷之間存在顯著的負(fù)相關(guān)(r=-0.248~-0.832,p0.0001):葉酸在30nM時(shí),遺傳 損傷均顯著高于120nM及240nM (p0.001～0.05):而120nM及240nM兩測試 組之間未觀察到各指標(biāo)間的統(tǒng)計(jì)學(xué)差異,當(dāng)葉酸升至120nM時(shí),遺傳損傷降到 最低,提示在本試驗(yàn)條件下,120nM的葉酸是防范人類淋巴細(xì)胞遺傳損傷的最
[Abstract]:Folic acid is an important micronutrient in the normal development of human body. It involves the synthesis of dUMP to dTMP.Synthesis of methionine (Met) from homocysteine (HC1) and synthesis of S- adenosine methionine (SAM)The DNA methylation was affected by the process of DNA methylation.So, folic acid, vitamin B, and Met/SAM.Blocked synthesis can cause dUMP accumulation and incorporation into DNA, resulting in various DNA structural damage.It can affect the process of DNA methylation in cells, thus affecting gene expression and chromosome separation.In this study, cytoplasmic block micronucleus analysis (CBMN) was used to study the effects of folic acid on the maintenance of breast cancer.The effects and differences of genomic stability of lymphocytes in control individuals were discussed, and the weight of folic acid metabolism pathway was also discussed.Lack of lymphocytes in breast cancer patients with BRCAI mutation and normal human lymphoid cellsGenotoxic effect of Barr cell line.In this study, lymphocytes were isolated from peripheral blood of 24 breast cancer patients and 30 normal controls.Study on the RPMI-1640 medium of 30nMN 120nM 240nM folic Acid with 8% Dialysis calf Serum 100 UThe cells were blocked by cytochalasin B (CBB) on the 8th day after culture with double antibody 2mm glutamine (pH7.0).Cells were harvested after 28 hours of cytokinesis, and CMBN was used to analyze the effects of folic acid deficiency on human lymphocyte production.Toxic effect: in order to confirm the effect of Metabac 12) on folic acid metabolism and genetic stability, the experiment also used 1550.渭 M Met and 751200pM BX 12) 12 kinds of RPMI-1640 medium containing 8% dialyzed calf serum.100U double antibody 2mm glutamine, pH7.0, cultured with BRCAI, gene mutation in breast cancer patientsLymphoblast cell line GM13705 and normal human lymphoblast cell line GM12593 were treated with cytochalasin on the 8th day.After 28 hours of blocking cytokinesis, the cells were harvested by BCBC, and CMBN analysis was used to analyze the effect of BCBI on human.Effect of Genomic Stability of Lymphocyte strain.The results show that:1. In the range of concentration in this study, folic acid concentration and genetic damage of lymphocytes in breast cancer patients and their controlsThere was a significant negative correlation between injury and injury: folic acid was inherited in 30nM.The damage was significantly higher than that of 120nM and 240nM (P 0.001 0. 05). 120nM and 240nM were the two tests.There was no statistical difference between the two groups. When folic acid rose to 120nM, the genetic damage decreased toThe results suggest that the folic acid of 120nM is the most important to prevent genetic damage of human lymphocytes.
【學(xué)位授予單位】：云南師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：Q987

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 呂嬌健,雷李美,王曉露;酒精性肝病患者血清葉酸與紅細(xì)胞體積分布圖變化的關(guān)系[J];現(xiàn)代診斷與治療;2000年05期

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本文編號(hào)：1759997