本文選題：四氯化碳 + 肝硬化��； 參考：《世界華人消化雜志》2014年01期

【摘要】：目的:觀察四氯化碳(CCl4)經(jīng)腹腔注射法制備肝硬化動物模型(cirrhosis model,CM)實驗中機體其他主要臟器的病理變化情況.方法:30只SD大鼠,隨機分為對照組和模型組.模型組:22只,使用橄欖油配備濃度為600mL/L的CCl4按3μL/g體質(zhì)量經(jīng)腹腔注射,3次/wk,共計12 wk,制備肝硬化大鼠模型(假小葉形成為判定CM成功的標(biāo)準(zhǔn));對照組:8只,按3L/g體質(zhì)量給予橄欖油溶液腹腔注射,3次/wk,共計12 wk.兩組大鼠均給予普通飼料加清水喂養(yǎng),并監(jiān)測實驗期間大鼠體質(zhì)量變化、活動及對外界的反應(yīng)情況,同時記錄大鼠的死亡情況及死亡時間.至12周末,模型組死亡6只,對照組全部存活.處死存活下來的模型組大鼠做肝臟病理,均符合肝硬化標(biāo)準(zhǔn).造模成功的同時收集食管、結(jié)腸、脾、肺等組織行HE染色,腎臟組織行PAS染色、并在光鏡下觀察各器官的組織形態(tài)學(xué)改變.結(jié)果:與對照組相比,模型組的大鼠肝組織呈典型的假小葉改變;食管、結(jié)腸可見少量的炎癥細(xì)胞浸潤,腎臟可見腎小管細(xì)胞輕度水腫.肺泡結(jié)構(gòu)破壞,部分區(qū)域內(nèi)有肺泡間隔繃緊,有多個肺泡的融合,肺泡腔內(nèi)見急性炎細(xì)胞及紅細(xì)胞.脾的組織形態(tài)基本正常.結(jié)論:本文首次對CCl4腹腔注射法制備肝硬化動物模型的重要臟器進(jìn)行病理學(xué)觀察,證明該方法制備的肝硬化大鼠模型對主要臟器的影響小,與人類發(fā)生肝硬化時重要臟器的病理改變基本一致,因此,再次說明該方法是一種理想的實驗性肝硬化動物模型的制備方法.
[Abstract]:Aim: to observe the pathological changes of other major organs in cirrhosis modelCM-induced cirrhosis model of liver cirrhosis by intraperitoneal injection of CCL _ 4 (CCL _ 4).Methods 30 Sprague-Dawley rats were randomly divided into control group and model group.In the model group, 22 rats were treated with CCl4 with olive oil concentration of 600mL/L at 3 渭 L / g body mass through intraperitoneal injection for 3 times / wk for a total of 12 wks. the model of cirrhotic rats was established (pseudolobules formation was the criterion for CM success; control group: 8 rats).Olive oil solution was intraperitoneally injected with olive oil solution for 12 wks. according to the body mass of 3L/g.The rats in both groups were fed with common diet with clear water, and the changes of body mass, activity and reaction to the outside world were monitored during the experiment. The death and time of death of rats were recorded at the same time.At the end of 12 weeks, 6 rats died in the model group and the control group survived.The surviving model rats were killed to make liver pathology, all of which were in accordance with the liver cirrhosis standard.The tissues of esophagus, colon, spleen and lung were collected for HE staining and PAS staining for renal tissue. The histomorphology of each organ was observed under light microscope.Results: compared with the control group, the liver tissue of the model group showed typical pseudolobular changes, a small number of inflammatory cells infiltrated in the esophagus and colon, and slight edema of renal tubule cells in the kidney.The alveolar structure was destroyed, alveolar septum taut in some areas, multiple alveolar fusion, acute inflammatory cells and erythrocytes were found in alveolar cavity.The shape of spleen was normal.Conclusion: the pathological observation on the important organs of the animal model of liver cirrhosis made by intraperitoneal injection of CCl4 was carried out for the first time, which proved that the rat model of liver cirrhosis made by this method had little effect on the main organs.This method is consistent with the pathological changes of important organs in human cirrhosis. Therefore, this method is an ideal method for the preparation of animal models of experimental liver cirrhosis.
【作者單位】： 中國人民解放軍總醫(yī)院南樓臨床部消化內(nèi)科;中國人民解放軍總醫(yī)院老年醫(yī)學(xué)研究所病理科;中國人民解放軍濟(jì)南軍區(qū)第二療養(yǎng)院;
【基金】：國家自然科學(xué)基金資助項目,No.30900669 北京市科技新星計劃基金資助項目~~
【分類號】：R-332

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