發(fā)布時(shí)間：2018-04-11 04:26

  本文選題：耐甲氧西林金黃色葡萄球菌 + β-內(nèi)酰胺酶　； 參考：《四川大學(xué)》2006年碩士論文

【摘要】：目的：探討耐甲氧西林金黃色葡萄球菌的輔助基因femA是否存在調(diào)控基因，以進(jìn)一步揭示其耐藥機(jī)制。 方法：用MIC法篩選對(duì)苯唑西林的高耐株、低耐株、敏感株；用Nitrocephin紙片法篩選出不產(chǎn)β-內(nèi)酰胺酶菌株；用PCR篩選出含mecA的菌株。將含mecA不產(chǎn)β-內(nèi)酰胺酶的高耐株、低耐株、敏感株作為進(jìn)一步研究材料。 PCR擴(kuò)增femA及fmA5′上游基因；提取細(xì)菌總蛋白；生物素標(biāo)記femA上游DNA序列作為探針；滯留電泳檢測(cè)femA調(diào)控基因與蛋白質(zhì)的相互作用。 結(jié)果：在198株金黃色葡萄球菌中對(duì)苯唑西林高度耐藥不產(chǎn)β-內(nèi)酰胺酶含mecA基因菌株有55株；對(duì)苯唑西林低度耐藥不產(chǎn)β-內(nèi)酰胺酶含mecA基因菌株有5株；滯留電泳檢測(cè)對(duì)苯唑西林高度耐藥不產(chǎn)β-內(nèi)酰胺酶含mecA基因菌株均出現(xiàn)滯留條帶，，即發(fā)現(xiàn)femA上游DNA序列與苯唑西林高度耐藥不產(chǎn)β-內(nèi)酰胺酶的菌株耐受相關(guān)蛋白有相互作用。 結(jié)論：耐甲氧西林金黃色葡萄球菌的輔助基因femd有調(diào)控基因存在的可能性。作用的機(jī)理可能是一種正反饋模式。
[Abstract]:Aim: to investigate the existence of regulatory gene in methicillin-resistant Staphylococcus aureus cogene femA in order to reveal its mechanism of drug resistance.Methods: the high and low resistant strains of oxacillin were screened by MIC, the strains without 尾 -lactamase were screened by Nitrocephin disk method, and the strains containing mecA were screened by PCR.High resistant strains, low resistant strains and sensitive strains containing mecA did not produce 尾 -lactamases.The upstream genes of femA and fmA5'were amplified by PCR; the total bacterial protein was extracted; the upstream DNA sequence of femA was labeled with biotin as a probe; and the interaction between femA regulatory gene and protein was detected by stranded electrophoresis.Results: among the 198 strains of Staphylococcus aureus, 55 strains with high resistance to oxacillin did not produce 尾 -lactamases and 5 strains with mecA gene did not produce 尾 -lactamases.Stranded electrophoresis was used to detect the presence of stranded bands of 尾 -lactamases with high resistance to oxacillin and no 尾 -lactamases. It was found that the upstream DNA sequence of femA interacted with the tolerance related proteins of those strains that were not highly resistant to oxacillin producing 尾 -lactamases.Conclusion: the cogene femd of methicillin-resistant Staphylococcus aureus has the possibility of regulating genes.The mechanism of action may be a positive feedback mode.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R378

【參考文獻(xiàn)】

相關(guān)期刊論文 前5條

1 丁玉仙;41株金黃色葡萄球菌的耐藥性分析[J];包頭醫(yī)學(xué)院學(xué)報(bào);2002年02期

2 徐修禮,于文彬,孫怡群,彭道榮,蘇明權(quán),馬越云;耐甲氧西林凝固酶陰性葡萄球菌的耐藥性[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2000年10期

3 周婭,趙忠,趙舒斌,王寧萍;金黃色葡萄球菌耐藥表型檢測(cè)[J];寧夏醫(yī)學(xué)雜志;2000年02期

4 李家泰 ,李耘 ,王進(jìn);中國(guó)醫(yī)院和社區(qū)獲得性感染革蘭陽(yáng)性球菌耐藥性監(jiān)測(cè)研究[J];中華醫(yī)學(xué)雜志;2003年05期

5 張慧琳,馮萍,呂曉菊,俞汝佳,周志強(qiáng),朱淑媛,陳文昭,陶傳敏,胥勁;萬(wàn)古霉素等11種抗菌藥物對(duì)葡萄球菌的體外抗菌活性[J];中國(guó)抗生素雜志;1998年06期

相關(guān)會(huì)議論文 前1條

1 陳智鴻;范昕建;呂曉菊;;mecA、femA基因PCR聯(lián)合擴(kuò)增法檢測(cè)MRSA[A];中華醫(yī)學(xué)會(huì)第七次全國(guó)感染病學(xué)術(shù)會(huì)議論文匯編[C];2001年

本文編號(hào)：1734438