本文選題：大鼠　切入點(diǎn)：足厥　出處：《中國協(xié)和醫(yī)科大學(xué)》2006年碩士論文

【摘要】：研究背景：經(jīng)絡(luò)學(xué)說是中華文明的瑰寶，而以此理論為基礎(chǔ)的針灸療法更是祖國中醫(yī)學(xué)的一技奇葩。然而經(jīng)絡(luò)的生物學(xué)性質(zhì)和形態(tài)學(xué)基礎(chǔ)是什么?如何解釋循經(jīng)感傳現(xiàn)象，至今仍未有系統(tǒng)的，有說服力的實(shí)驗(yàn)依據(jù)。本研究力求從現(xiàn)代醫(yī)學(xué)神經(jīng)解剖學(xué)和神經(jīng)生理的角度揭示經(jīng)絡(luò)和穴位的奧秘。進(jìn)一步探討經(jīng)絡(luò)和穴位的形態(tài)學(xué)基礎(chǔ)和神經(jīng)生物學(xué)機(jī)制。研究方法：本研究采用霍亂毒素B亞單位結(jié)合辣根過氧化物酶(CB-HRP)逆行示蹤法，研究支配大鼠足厥陰肝經(jīng)及足少陰腎經(jīng)所屬肌肉的運(yùn)動神經(jīng)元在脊髓前角內(nèi)的分布，觀察同經(jīng)運(yùn)動神經(jīng)元間的樹突投射聯(lián)系，同時，研究足少陰腎經(jīng)和足陽明胃經(jīng)，共同走行的腹直肌運(yùn)動神經(jīng)元在脊髓前角的分布，觀察其運(yùn)動神經(jīng)元間樹突投射的聯(lián)系。研究結(jié)果：1．CB-HRP逆行追蹤顯示，，足厥陰肝經(jīng)和足少陰腎經(jīng)相關(guān)的運(yùn)動神經(jīng)元在脊髓的空間排布：在脊髓縱(水平)切片上標(biāo)記的細(xì)胞形成一個首尾走向的運(yùn)動神經(jīng)元柱，而同一細(xì)胞柱之間存在豐富的樹突—樹突和樹突—胞體投射，其中足厥陰肝經(jīng)標(biāo)記神經(jīng)元首尾分部在L_2—S_1脊髓節(jié)段長度為7.0±0.5mm，相互投射的樹突數(shù)占樹突總數(shù)的71.6％。足少陰腎經(jīng)標(biāo)記神經(jīng)元首尾分兩部分，分別在T_8—T_(12)和L_3—S_2脊髓節(jié)段，長度分別為10.6±0.6mm和4.1±0.8mm。相互投射的樹突數(shù)占樹突總數(shù)的75.6％。在脊髓的橫切片上，標(biāo)記的神經(jīng)元足厥陰肝經(jīng)主要集中在L_2—L_5腹角前外側(cè)核，且有運(yùn)動神經(jīng)元的突起向側(cè)角的位置分布；足少陰腎經(jīng)主要集中在T_8—T_(12)和L_3—S_2兩個脊髓節(jié)段，其中可觀察到有運(yùn)動神經(jīng)元的突起向側(cè)角或相當(dāng)于側(cè)角及中間帶第二內(nèi)臟灰質(zhì)和中央管周灰質(zhì)后連合核及脊髓軟膜下的投射，以T_8—T_(12)節(jié)段最為明顯。此外，在T_8—T_(12)節(jié)段還可見中央管周圍灰質(zhì)、后角、中間帶均有大量順行標(biāo)記，某幾個節(jié)段還可見胸核的大量順行標(biāo)記。2．足少陰腎經(jīng)及足陽明胃經(jīng)走行的腹直肌運(yùn)動神經(jīng)元在脊髓的空間排布：水平切面標(biāo)記運(yùn)動神經(jīng)元分布在T_7—T_(12)節(jié)段，首尾走向長度為10.8±0.5mm，標(biāo)記細(xì)胞的密度主要集中在T_8—T_(10)節(jié)段，神經(jīng)元為一條縱行柱，核柱之間有豐富的樹—樹和樹—體投射。相互投射的樹突數(shù)占樹突總數(shù)的72.2％。橫切面上的結(jié)果與足少陽腎
[Abstract]:Background: meridian theory is a treasure of Chinese civilization, and acupuncture therapy based on this theory is a technical work of traditional Chinese medicine.However, what are the biological properties and morphological basis of meridian?How to explain the phenomenon of sensory transmission along the meridian, so far there is no systematic, persuasive experimental basis.This study seeks to reveal the mysteries of meridians and acupoints from the perspective of modern medical neuroanatomy and neurophysiology.To further explore the morphological basis and neurobiological mechanism of meridians and acupoints.Methods: the distribution of motoneurons in the anterior horn of spinal cord was studied by the retrograde tracing method of cholera toxin B subunit B and horseradish peroxidase (horseradish peroxidase) in the muscles of rat foot Jueyin liver meridian and foot Shaoyin kidney meridian.At the same time, the distribution of the motor neurons of rectus abdominis in the anterior horn of spinal cord and the relation of dendritic projection between the motoneurons and the meridian meridian and the stomach meridian of foot yang Ming were studied in order to observe the relation of dendritic projection between the motoneurons and the motoneurons of the motoneurons.Results 1. The retrograde tracing of CB-HRP showed that the motor neurons associated with the liver meridian and the kidney meridian of foot Jueyin were arranged in the space of the spinal cord: the cells labeled on the longitudinal (horizontal) sections of the spinal cord formed a motoneuronic column along the tail and head.The labeled neurons were divided into two parts, the length of which was 10.6 鹵0.6mm and 4.1 鹵0.8 mm. respectively in T _ s _ (8-T _ (12)) and L_3-S_2 spinal cord segments.The number of dendrites projecting to each other accounts for 75.6% of the total dendrites.In the transverse section of spinal cord, the labeled neurons were mainly located in the anterolateral nucleus of the ventral horn of L_2-L_5, and there was the distribution of the process of motor neurons to the lateral horn.The kidney meridian of foot little yin is mainly concentrated in the two segments of the spinal cord of T _ S _ 8-T _ T _ I _ 12) and L_3-S_2.The projections of the protuberance to the lateral horn of motor neurons or corresponding to the lateral horn and the second visceral gray matter of the intermediate band and the posterior commissural nucleus of the central periductal gray matter and the subpial spinal cord were observed, especially in the T _ 8-T _ T _ (12) segment.In addition, in the T _ 8-T _ (12) segment, the gray matter around the central canal, the posterior horn and the middle band were all marked with a large number of anterograde markers, and in some segments, a large number of anterograde marks of the thoracic nucleus could be seen.The neurons are a longitudinal column with abundant tree-tree and tree-body projections between the nuclear pillars.The number of dendrites projecting to each other accounts for 72.2% of the total dendrites.Cross-sectional results and foot Shaoyang Kidney
【學(xué)位授予單位】：中國協(xié)和醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R322;R224.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 汪克明,周逸平,周美啟,王月蘭,陳業(yè)農(nóng);電針心經(jīng)、小腸經(jīng)對急性心肌缺血大鼠心功能的影響[J];安徽中醫(yī)學(xué)院學(xué)報;2003年04期

2 張人驥,潘其麗;經(jīng)絡(luò)科學(xué)論[J];北京大學(xué)學(xué)報(自然科學(xué)版);2003年01期

3 劉里遠(yuǎn)，郝春杰，樊景禹;皮膚中兒茶酚胺類體液物質(zhì)及毛囊與針刺效應(yīng)的關(guān)系[J];北京師范大學(xué)學(xué)報(自然科學(xué)版);1996年04期

4 趙平平;建國以來“經(jīng)絡(luò)的實(shí)質(zhì)”研究概況[J];中國醫(yī)藥學(xué)報;2001年03期

5 劉芳,陳爾瑜,黨瑞山,承煥生;胃經(jīng)沿線小腿骨間膜的血管及鈣的分布[J];第二軍醫(yī)大學(xué)學(xué)報;2000年08期

6 陳道亮,萬隆,謝建珍,吳寶華,胡翔龍;人胚胎發(fā)育中表皮細(xì)胞連接通訊沿經(jīng)連續(xù)性的建立[J];福建中醫(yī)學(xué)院學(xué)報;2000年01期

7 鐘析;三焦經(jīng)、膽經(jīng)與植物神經(jīng)的相關(guān):基于井穴刺絡(luò)學(xué)的觀點(diǎn)[J];國外醫(yī)學(xué)(中醫(yī)中藥分冊);1994年01期

8 符海;針灸刺激外周機(jī)制的多覺型感受器假說[J];國外醫(yī)學(xué)(中醫(yī)中藥分冊);1994年03期

9 徐春軍,陸靜;經(jīng)驗(yàn)醫(yī)術(shù)的科學(xué)——正確繼承和發(fā)展傳統(tǒng)醫(yī)學(xué)[J];國外醫(yī)學(xué)(中醫(yī)中藥分冊);1996年06期

10 陳丁生;明治針灸大學(xué)針灸研究概況[J];國外醫(yī)學(xué)(中醫(yī)中藥分冊);1997年04期

本文編號：1723067