本文選題：子宮內(nèi)膜　切入點(diǎn)：蛻膜化　出處：《大連醫(yī)科大學(xué)》2005年碩士論文

【摘要】：目的:探討精液成分對(duì)子宮內(nèi)膜蛻膜化及胚胎發(fā)育的影響。 方法:1.分離育齡期婦女增殖晚期及分泌期子宮內(nèi)膜細(xì)胞,加入雌二醇(E2)、孕酮(P)使其蛻膜化,分別加入人精子及精漿共培養(yǎng),觀察蛻膜化的間質(zhì)細(xì)胞生長情況并測(cè)定培養(yǎng)液中泌乳素PRL 含量。2.內(nèi)膜培養(yǎng)8 天后,加入小鼠2-細(xì)胞期胚胎繼續(xù)培養(yǎng),觀察胚胎的發(fā)育情況及內(nèi)膜細(xì)胞生長情況。3.建立假孕鼠模型,觀察精液在胚胎移植中的作用。 結(jié)果:1.加入精漿的子宮內(nèi)膜間質(zhì)細(xì)胞蛻膜化程度明顯優(yōu)于精子組和對(duì)照組。精漿組分泌PRL 的量與精子組和對(duì)照組相比有顯著性差異(p0.05),而精子組和對(duì)照組無明顯差異。2.精漿能促進(jìn)八細(xì)胞以后的胚胎在子宮內(nèi)膜細(xì)胞上的卵裂、囊胚形成、孵化、黏附和擴(kuò)展性生長(p0.05)。3. 胚胎移植過程中精子對(duì)植入率沒有影響。 結(jié)論:1 精漿對(duì)子宮內(nèi)膜間質(zhì)細(xì)胞蛻膜化有明顯促進(jìn)作用,能夠促進(jìn)PRL 的分泌。2.精漿的存在可以促進(jìn)胚胎在子宮內(nèi)膜細(xì)胞共培養(yǎng)體系中的發(fā)育。3.以輸卵管結(jié)扎和假孕鼠為受體的胚胎移植著床率無明顯差異提示了精漿與精液對(duì)囊胚著床作用可能相同。
[Abstract]:Objective: to investigate the effect of semen composition on decidualization and embryo development of endometrium.Method 1: 1.Endometrial cells of late proliferative and secretory phase were isolated from women of childbearing age. The decidua cells were treated with estradiol E2P and progesterone. The cells were co-cultured with human spermatozoa and seminal plasma, respectively.The growth of decidualized mesenchymal cells was observed and the content of prolactin PRL in culture medium was determined.After 8 days of endometrial culture, mouse 2-cell embryos were added to continue to culture, and the development of embryo and the growth of endometrial cells were observed.The role of semen in embryo transfer was observed by establishing pseudopregnant mouse model.The result is 1: 1.The degree of decidualization of endometrial stromal cells added seminal plasma was better than that of spermatozoa group and control group.The amount of PRL secreted in seminal plasma group was significantly different from that in spermatozoa group and control group (p0.05), but there was no significant difference between spermatozoa group and control group.Seminal plasma can promote the cleavage, blastocyst formation, hatching, adhesion and extensibility of the embryo in endometrial cells.Spermatozoa have no effect on implantation rate during embryo transfer.Conclusion the seminal plasma of 1: 1 can promote decidualization of endometrial stromal cells and promote the secretion of PRL.The existence of seminal plasma can promote the development of embryo in the co-culture system of endometrial cells.There was no significant difference in implantation rate between oviduct ligation and pseudopregnant mice, suggesting that seminal plasma and semen might have the same effect on blastocyst implantation.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R321

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 駱建民;鄭玉鸞;周元聰;柯慧心;周白菡;;金黃地鼠腹側(cè)前列腺來源蛋白結(jié)合精子表面的實(shí)驗(yàn)研究[J];中華男科學(xué)雜志;2007年10期

2 駱建民;謝江新;王俐;;前列腺精子結(jié)合蛋白的二維凝膠電泳及質(zhì)譜分析[J];中山大學(xué)學(xué)報(bào)(醫(yī)學(xué)科學(xué)版);2007年06期

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本文編號(hào)：1703727