本文選題：糞腸球菌　切入點(diǎn)：利奈唑胺　出處：《中國熱帶醫(yī)學(xué)》2014年11期 　論文類型：期刊論文

【摘要】：目的研究1例肺部感染患者體內(nèi)利奈唑胺耐藥糞腸球菌株突變以及定植特點(diǎn)。方法從1例反復(fù)發(fā)作肺炎(1年內(nèi)反復(fù)發(fā)作5次)的急性白血病患者的氣管分泌物中分離出10株糞腸球菌株,菌株的MIC值通過E-test測定,經(jīng)PCR擴(kuò)增細(xì)菌4個(gè)拷貝23S r RNA基因V區(qū)基因,測序分析突變位點(diǎn),通過脈沖場凝膠電泳(PFGE)觀察分離菌株同源性。結(jié)果患者5次肺炎發(fā)作從氣管分泌物中分離的10株糞腸球菌株均為利奈唑胺不敏感株(4株為中介耐藥株,6株為耐藥株),通過PCR和測序發(fā)現(xiàn)4株利奈唑胺中介耐藥株含有1個(gè)拷貝23S r RNA基因V區(qū)G2424U突變,而利奈唑胺耐藥菌株同時(shí)含有G2424U和G2576U雙突變。通過PFGE分型檢測提示10株糞腸球菌株具有同源性。通過肉湯稀釋法鑒定這些菌株均對(duì)萬古霉素敏感。結(jié)論利奈唑胺耐藥糞腸球菌株可能長期定植于患者體內(nèi),并導(dǎo)致反復(fù)感染。
[Abstract]:Objective to study the mutagenesis and colonization characteristics of lenazolidomide resistant Enterococcus faecalis strain in a patient with pulmonary infection. Methods the tracheal secretion of a patient with acute leukemia with recurrent pneumonia (5 episodes in one year) was studied. Ten strains of Enterococcus faecalis were isolated. The MIC value of the strain was determined by E-test. The V region of 23S r RNA gene was amplified by PCR, and the mutation site was analyzed by sequencing. Results 10 strains of Enterococcus faecalis isolated from trachea secretions in 5 episodes of pneumonia were all linazolamide insensitive strains and 4 strains were intermediate resistance strains. By PCR and sequencing, it was found that there was one copy of G2424U mutation in V region of 23S r RNA gene. Both G2424U and G2576U were found in linazolamide resistant strains. PFGE typing showed that 10 strains of Enterococcus faecalis were homologous. These strains were all sensitive to vancomycin by broth dilution method. Conclusion Liniazolamide is sensitive to vancomycin. The drug-resistant Enterococcus faecalis strain may be colonized in the patient for a long time. And lead to repeated infections.
【作者單位】： 廣東醫(yī)學(xué)院附屬深圳市南山區(qū)人民醫(yī)院;
【基金】：深圳市南山區(qū)衛(wèi)生科技計(jì)劃項(xiàng)目(No.2012028,2012021) 深圳市科技創(chuàng)新-知識(shí)創(chuàng)新計(jì)劃項(xiàng)目(No.45584896-4)
【分類號(hào)】：R378.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 羽曉瑜;陸玉霞;;糞腸球菌聚集物質(zhì)的研究進(jìn)展[J];中國微生態(tài)學(xué)雜志;2010年07期

2 潘偉光;李一凡;鄧啟文;張健華;;1株利奈唑胺耐藥糞腸球菌的基因突變檢測[J];中國感染與化療雜志;2013年02期

3 吳利先,黃文祥;糞腸球菌sprE基因的功能研究[J];中華微生物學(xué)和免疫學(xué)雜志;2005年10期

4 孫小平;黃文祥;吳利先;;糞腸球菌基因hyl_1功能的實(shí)驗(yàn)研究[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2007年14期

5 狄婷婷;高原;;糞腸球菌主要毒力因子研究進(jìn)展[J];中國病原生物學(xué)雜志;2012年03期

6 李春,張丹,張?zhí)m威,于維軍;兩株降膽固醇球菌生長條件的研究[J];現(xiàn)代食品科技;2005年04期

7 董明;;饑餓期糞腸球菌生物膜毒力因子明膠酶E表達(dá)研究[J];口腔生物醫(yī)學(xué);2013年02期

8 董明;王珊珊;王麗娜;牛衛(wèi)東;;饑餓期糞腸球菌生物膜毒力因子明膠酶E表達(dá)研究[J];實(shí)用口腔醫(yī)學(xué)雜志;2012年05期

9 武楠;顏向軍;劉亞琴;鄒禮衡;楊祚升;;糞腸球菌Ace重組蛋白的表達(dá)、純化及黏附活性研究[J];微生物學(xué)雜志;2008年03期

10 李曉玉;丁鵬;韓傳銀;米志強(qiáng);安小平;黃勇;鄭旺良;馮福民;童貽剛;;糞腸球菌噬菌體IME-EF3的分離及其治療優(yōu)勢的發(fā)掘[J];生物醫(yī)學(xué)工程與臨床;2014年02期

相關(guān)會(huì)議論文 前6條

1 劉紅艷;韋曦;凌均h，

本文編號(hào)：1631279