本文選題：tat　切入點(diǎn)：HIV-1　出處：《吉林大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

【摘要】：DNA 疫苗研究的一個重要任務(wù)是如何能更好地提高DNA 疫苗的體液免疫和細(xì)胞免疫。近年來的研究顯示,HIV 的轉(zhuǎn)錄活化因子—Tat 蛋白具有自由通過細(xì)胞質(zhì)膜的特性�，F(xiàn)已有不少借助Tat 蛋白的穿膜活性將其它蛋白或DNA 成功導(dǎo)入活細(xì)胞內(nèi)的報道。根據(jù)大量文獻(xiàn)提供的理論基礎(chǔ)設(shè)計了實(shí)驗,將tat 與gag/pol 融合表達(dá),利用Tat 蛋白的穿膜活性將gag/pol 蛋白導(dǎo)入其它活細(xì)胞內(nèi),從而提高DNA 疫苗免疫水平。還有研究表明Tat49-57 短肽T9(RKKRRQRRR)有較強(qiáng)的穿膜功能,因此我們將這T9 與gag/pol融合表達(dá),研究T9 的插入對DNA 疫苗的免疫水平的影響。 本論文的主要工作是構(gòu)建DNA 疫苗,將tat、T9 與HIV-1gag/pol 融合表達(dá),檢測DNA 疫苗的抗原性和免疫原性,研究Tat、T9 蛋白對提高HIV-1 DNA 疫苗免疫水平的作用。實(shí)驗結(jié)果證明tat和gag/pol 融合表達(dá)能提高DNA 疫苗的細(xì)胞免疫水平。Tat 基因的插入提高HIV-1 DNA 疫苗的有效性,也為tat 應(yīng)用于其他疫苗中提供了廣闊實(shí)際應(yīng)用前景。 實(shí)驗中我們發(fā)現(xiàn)T9 與gag/pol 的融合表達(dá)并不是象我們預(yù)期設(shè)想那樣能提高HIV-1 DNA 疫苗的免疫水平,反而有所降低,我們將在以后的實(shí)驗中進(jìn)一步分析研究其原因。
[Abstract]:One of the important tasks of DNA vaccine research is how to improve the humoral and cellular immunity of DNA vaccine. Recent studies have shown that the transcriptional activator Tat protein of DNA has the property of free passing through the cytoplasmic membrane. There are many reports that other proteins or DNA have been successfully introduced into living cells by the transmembrane activity of Tat protein. Experiments have been designed based on the theoretical basis provided by a large number of literatures. The expression of tat and gag/pol was fused, and the gag/pol protein was introduced into other living cells by the transmembrane activity of Tat protein, so as to improve the immune level of DNA vaccine. Studies also showed that Tat49-57 short peptide T9 RKKRRQRRRRR) had strong transmembrane function, so we fused the expression of T9 with gag/pol. To study the effect of T 9 insertion on the immune level of DNA vaccine. The main work of this thesis is to construct DNA vaccine, to express T9 with HIV-1gag/pol, and to detect the antigenicity and immunogenicity of DNA vaccine. To study the effect of Tatt T9 protein on improving the immune level of HIV-1 DNA vaccine. The results show that the fusion expression of tat and gag/pol can improve the cellular immunity level of DNA vaccine. Tat gene insertion can improve the effectiveness of HIV-1 DNA vaccine. It also provides a broad prospect for the application of tat in other vaccines. In our experiment, we found that the fusion expression of T9 and gag/pol did not increase the immune level of HIV-1 DNA vaccine as we expected, but decreased, and we will further analyze and study its cause in future experiments.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R392

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